Nelumbo nucifera leaf extracts inhibit the formation of advanced glycation end-products and mechanism revealed by Nano LC-Orbitrap-MS/MS

2018 ◽  
Vol 42 ◽  
pp. 254-261 ◽  
Author(s):  
Lu Zhang ◽  
Chong-ju Zhang ◽  
Zong-cai Tu ◽  
Wen-hua Yang ◽  
Yi Zhao ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Nelumbo Nucifera ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Orbitrap Ms

scholarly journals Improved Methods for the Rapid Formation and Prevention of Advanced Glycation End Products (AGEs) In Vitro by Coupling to the Hypoxanthine/Xanthine Oxidase Assay System

Biomedicines ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 88 ◽  
Author(s):  
Samuel Marques ◽  
Teresa Trevisan ◽  
Carlos Maia ◽  
Andrea Breuer ◽  
Robert Owen
Keyword(s):  
Xanthine Oxidase ◽  
Advanced Glycation End Products ◽  
High Capacity ◽  
Protein Glycation ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Reactive Carbonyl Species ◽  
Glycation End Products ◽  
End Products

Advanced glycation end products (AGEs) represent a set of molecules that contribute directly to the initiation and aggravation of diseases associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds), proteins, and amino acid residues. Previous in vitro methods using non-enzymatic procedures described in the literature require an incubation period of 1–3 weeks to generate AGEs. In this study, the reaction time for the formation of AGEs (48 and 3 h) was significantly reduced by adaptation of methods previously described in the literature and coupling them to the free radical generation system termed hypoxanthine/xanthine oxidase assay. The incorporation of this assay into the experimental system accelerated the production of AGEs as a result of the formation of reactive oxygen species (ROS), as shown by increased fluorescence. The capacity of different classes of chemical compounds (aminoguanidine, chlorogenic acid, rutin, and methanol extracts of Hancornia speciosa Gomes) to inhibit protein glycation by acting as scavenging agents of α-dicarbonyl species was evaluated. Aminoguanidine and, especially, rutin identified in the leaf extracts of H. speciosa Gomes showed a high capacity to act as scavengers of reactive carbonyl species RCS-trapping, resulting in the inhibition of AGEs formation.

scholarly journals Inhibitory Effect of Several Mangifera indica Cultivar Leaf Extracts on the Formation of Advanced Glycation End Products (AGEs)

2020 ◽  
Vol 9 (2) ◽  
pp. 33
Author(s):  
Kimihisa Itoh ◽  
Tetsuya Matsukawa ◽  
Kanasa Minami ◽  
Mamoru Okamoto ◽  
Norimichi Tomohiro ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Mangifera Indica ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Indica Cultivar

As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation.

scholarly journals Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract

2017 ◽  
Vol 6 (2) ◽  
pp. 102 ◽  
Author(s):  
Kimihisa Itoh ◽  
Kazuya Murata ◽  
Nao Sakaguchi ◽  
Kohei Akai ◽  
Tomoka Yamaji ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Mangifera Indica ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Dark Green

The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.

scholarly journals New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System

2018 ◽  
Author(s):  
Samuel Marques ◽  
Teresa Trevisan ◽  
Carlos Maia ◽  
Andrea Breuer ◽  
Robert Owen
Keyword(s):  
Xanthine Oxidase ◽  
Advanced Glycation End Products ◽  
High Capacity ◽  
Protein Glycation ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Hancornia Speciosa

Advanced Glycation End Products (AGEs) represent a set of substances that contribute directly to the triggering and/or aggravation of pathologies associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds) proteins and amino acid residues. Current methodologies require an incubation period of 1-3 weeks to generate AGEs. In this study the reaction time for the formation of AGEs (48 and 3 hours) is significantly reduced by coupling and adapting procedures already existing in the literature to the free radical generation system called the hypoxanthine/xanthine oxidase assay. The capacity of different classes and chemical compounds (aminoguanidine, chlorogenic acid, rutin, extracts of Hancornia speciosa Gomes) were evaluated to inhibit the protein glycation process, acting as capturing agents of α-dicarbonyl species. Aminoguanidine, rutin and the leaf extracts of Hancornia speciosa Gomes show a high capacity to act as α-dicarbonyl compound scavengers (RCS-trapping) and resulting in the inhibition of AGEs formation.

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