Non-extractable polyphenols of green tea and their antioxidant, anti-α-glucosidase capacity, and release during in vitro digestion

2018 ◽  
Vol 42 ◽  
pp. 129-136 ◽  
Author(s):  
Shuaishuai Yan ◽  
Hongjun Shao ◽  
Zhihao Zhou ◽  
Qi Wang ◽  
Luhua Zhao ◽  
...  
Keyword(s):  
Green Tea ◽  
In Vitro Digestion

Milk enhances intestinal absorption of green tea catechins in in vitro digestion/Caco-2 cells model

2013 ◽  
Vol 53 (2) ◽  
pp. 793-800 ◽  
Author(s):  
Yanlan Xie ◽  
Agnieszka Kosińska ◽  
Hairong Xu ◽  
Wilfried Andlauer
Keyword(s):  
Intestinal Absorption ◽  
Green Tea ◽  
In Vitro Digestion ◽  
Tea Catechins ◽  
Green Tea Catechins

Variability in the Antioxidant Activity of Dietary Supplements from Pomegranate, Milk Thistle, Green Tea, Grape Seed, Goji, and Acai: Effects of in Vitro Digestion

2014 ◽  
Vol 62 (19) ◽  
pp. 4313-4321 ◽  
Author(s):  
Susanne M. Henning ◽  
Yanjun Zhang ◽  
Victoria G. Rontoyanni ◽  
Jianjun Huang ◽  
Ru-Po Lee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Dietary Supplements ◽  
Green Tea ◽  
In Vitro Digestion ◽  
Grape Seed ◽  
Milk Thistle

scholarly journals Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 663
Author(s):  
Ewa Habza-Kowalska ◽  
Urszula Gawlik-Dziki ◽  
Dariusz Dziki
Keyword(s):  
Green Tea ◽  
Radical Scavenging ◽  
Sinapinic Acid ◽  
In Vitro Digestion ◽  
Thyroid Peroxidase ◽  
Green Coffee ◽  
Lipoxygenase Inhibitors ◽  
Lox Inhibitors ◽  
Ethanol Extracts

This study focused on the effect of kaempferol, catechin, apigenin, sinapinic acid, and extracts from plants (i.e., parsley, cumin, mustard, green tea, and green coffee) on thyroid peroxidase (TPO) and lipoxygenase (LOX) activity, antiradical potential, as well as the result of interactions among them. Catechin, sinapinic acid, and kaempferol acted as a competitive TPO inhibitors, while apigenin demonstrated an uncompetitive mode of inhibitory action. Ethanol extracts from all plants acted as competitive TPO inhibitors, while, after in vitro digestion, TPO activation was found especially in the case of mustard (24%) and cumin (19.85%). Most importantly, TPO activators acted synergistically. The TPO effectors acted as LOX inhibitors. The most effective were potentially bioaccessible compounds from green tea and green coffee (IC50 = 29.73 mg DW/mL and 30.43 mg DW/mL, respectively). The highest free radical scavenging ability was determined for catechin and sinapinic acid (IC50 = 78.37 µg/mL and 84.33 µg/mL, respectively) and potentially bioaccessible compounds from mustard (0.42 mg DW/mL) and green coffee (0.87 mg DW/mL). Green coffee, green tea, cumin, and mustard contain potentially bioaccessible TPO activators that also act as effective LOX inhibitors, which indicate their potentially health-promoting effects for people suffering from Hashimoto’s disease.

Effects of iron, ascorbate, meat and casein on the antioxidant capacity of green tea under conditions of in vitro digestion

2006 ◽  
Vol 94 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Isidora Alexandropoulou ◽  
Michael Komaitis ◽  
Maria Kapsokefalou
Keyword(s):  
Antioxidant Capacity ◽  
Green Tea ◽  
In Vitro Digestion

scholarly journals Profiling of In Vitro Bioaccessibility and Intestinal Uptake of Flavonoids after Consumption of Commonly Available Green Tea Types

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1518
Author(s):  
Jeong-Ho Oh ◽  
Chan-Yang Lee ◽  
Yeong-Eun Lee ◽  
So-Hee Yoo ◽  
Jin-Oh Chung ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Green Tea ◽  
In Vitro Digestion ◽  
Model System ◽  
Intestinal Uptake ◽  
In Vitro Bioaccessibility

The aim of this study was to profile the bioaccessibility and intestinal absorption of epicatechins and flavonols in different forms of green tea and its formulation: loose leaf tea, powdered tea, 35% catechins containing GTE, and GTE formulated with green tea-derived polysaccharide and flavonols (CATEPLUS™). The bioaccessibillity and intestinal absorption of epicatechins and flavonols was investigated by using an in vitro digestion model system with Caco-2 cells. The bioaccessibility of total epicatechins in loose leaf tea, powdered tea, GTE, and CATEPLUS™ was 1.27%, 2.30%, 22.05%, and 18.72%, respectively, showing that GTE and CATEPLUS™ had significantly higher bioaccessibility than powdered tea and loose leaf tea. None of the flavonols were detected in powdered tea and loose leaf tea, but the bioaccessibility of the total flavonols in GTE and CATEPLUS™ was 85.74% and 66.98%, respectively. The highest intestinal absorption of epicatechins was found in CATEPLUS™ (171.39 ± 5.39 ng/mg protein) followed by GTE (57.38 ± 9.31), powdered tea (3.60 ± 0.67), and loose leaf tea (2.94 ± 1.03). The results from the study suggest that formulating green tea extracts rich in catechins with second components obtained from green tea processing could enhance the bioavailability of epicatechins.

In vitro antioxidant activities of Bauhinia strychnifolia extracts: Comparison with green tea extracts

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
A Itharat ◽  
S Sayompark ◽  
P Hansakul ◽  
B Dechayont
Keyword(s):  
Green Tea ◽  
Antioxidant Activities

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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