Effect of heat denaturation of egg white proteins ovalbumin and ovomucoid on CD4+ T cell cytokine production and human mast cell histamine production

2015 ◽  
Vol 18 ◽  
pp. 28-34 ◽  
Author(s):  
Prithy Rupa ◽  
Lindsay Schnarr ◽  
Yoshinori Mine
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
Cytokine Production ◽  
Egg White ◽  
Cd4 T Cell ◽  
Human Mast Cell ◽  
Heat Denaturation ◽  
Histamine Production ◽  
Egg White Proteins ◽  
Cell Cytokine Production

Preferential Uptake of Gamma Tocopherol by Mast Cells. Does This Uptake Affect Mast Cell Cytokine Production?.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3793-3793
Author(s):  
Omar S. Aljitawi ◽  
Mathew Fitzgerald ◽  
Min Qui ◽  
Koyamangalath Krishnan ◽  
William L. Stone ◽  
...  
Keyword(s):  
Mast Cell ◽  
Vitamin E ◽  
Mast Cells ◽  
Cytokine Production ◽  
Ppar Gamma ◽  
Alpha Tocopherol ◽  
Human Mast Cell ◽  
Peroxisome Proliferator ◽  
Gamma Tocopherol ◽  
Cell Cytokine Production

Abstract Vitamin E, the main lipid soluble antioxidant, exists in eight different forms, of which α-tocopherol and γ-tocopherol are the two major forms. Previous experiments showed vitamin E uptake by macrophages that contribute to inflammation and immunity. On the other hand, vitamin E has structural similarity to the thiazolidinedione, troglitazone, a peroxisome proliferator-activated receptor (PPAR) agonist. In previous experiments we found that troglitazone (TGZ), a PPAR gamma agonist, had a negative effect on mast cell cytokine production. We therefore wondered whether vitamin E enters human mast cells, and if so, does this modulate mast cell cytokine production? We choose (interleukin) IL-6 for its pro-inflammatory properties and because it’s known to be produced by mast cells in response to stimulants used in the experiment. In this study we try to answer these two questions. Cultured human mast cell line (HMC-1) was first incubated for 24 hrs with pharmacological concentrations of both alpha and gamma forms of vitamin E (10 μM). The mast cells were then activated with phorbol-12-myristate-13-acetate [PMA (50ng/ml)] and ionomycin (5μm) for 24 hours and cell-free supernatants collected. In additional experiments, IL-1ß (10ng/mL) was added to activate mast cells. IL-6 levels in the supernatants were determined in each well utilizing ELISA. Mast cell concentrations of alpha or gamma tocopherol were measured by high pressure liquid chromatography [HPLC] and electrochemical analysis. Mast cells pre-incubated in alpha and gamma forms of vitamin E at 10 μM did not affect mast cell IL-6 production. Mast cells, however, showed uptake of both forms of tocopherols but more pronounced uptake of the gamma form (13181.05 pmole/well of gamma compared to 8742.99 pmole/well of alpha tocopherol). We conclude that mast cells appear to store both alpha and gamma tocopherols but preferentially more gamma tocopherol. Though Vitamin E and PPAR agonists have similar structures, they did not show similar effect on mast cell cytokine production, suggesting they might have different mechanisms of action.

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ

2000 ◽  
Vol 106 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Hiroshi Tachimoto ◽  
Motohiro Ebisawa ◽  
Tomohide Hasegawa ◽  
Tomoko Kashiwabara ◽  
Chisei Ra ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cytokine Production ◽  
Human Mast Cell ◽  
Reciprocal Regulation ◽  
Ifn Γ ◽  
Cell Cytokine Production

scholarly journals Abdominal visceral adiposity influences CD4+ T cell cytokine production in pregnancy

Cytokine ◽  
2015 ◽  
Vol 71 (2) ◽  
pp. 405-408 ◽  
Author(s):  
Marlies K. Ozias ◽  
Shengqi Li ◽  
Holly R. Hull ◽  
William M. Brooks ◽  
Margaret G. Petroff ◽  
...  
Keyword(s):  
T Cell ◽  
Cytokine Production ◽  
Visceral Adiposity ◽  
Cd4 T Cell ◽  
Cell Cytokine Production ◽  
In Pregnancy

scholarly journals Adipocytes from the infrapatellar fat pad of OA patients modulate CD4+ T cell cytokine production

2011 ◽  
Vol 9 (S2) ◽  
Author(s):  
Andreea Ioan-Facsinay ◽  
Joanneke C Kwekkeboom ◽  
Margreet Kloppenburg ◽  
Rene EM Toes
Keyword(s):  
T Cell ◽  
Cytokine Production ◽  
Cd4 T Cell ◽  
Infrapatellar Fat Pad ◽  
Fat Pad ◽  
Cell Cytokine Production

scholarly journals Allergen-Induced CD4+ T Cell Cytokine Production within Airway Mucosal Dendritic Cell–T Cell Clusters Drives the Local Recruitment of Myeloid Effector Cells

2016 ◽  
Vol 198 (2) ◽  
pp. 895-907 ◽  
Author(s):  
Tibor Z. Veres ◽  
Tamás Kopcsányi ◽  
Nicholas van Panhuys ◽  
Michael Y. Gerner ◽  
Zhiduo Liu ◽  
...  
Keyword(s):  
T Cell ◽  
Dendritic Cell ◽  
Cytokine Production ◽  
Cd4 T Cell ◽  
Effector Cells ◽  
Cell Clusters ◽  
Local Recruitment ◽  
Cell Cytokine Production

CD4+ T cell cytokine production is influenced by jagged 1

2008 ◽  
Vol 22 (S2) ◽  
pp. 406-406
Author(s):  
Matthew A Schaller ◽  
Ana L Coelho ◽  
Nicholas W Lukacs ◽  
Steven L Kunkel
Keyword(s):  
T Cell ◽  
Cytokine Production ◽  
Cd4 T Cell ◽  
Cell Cytokine Production

scholarly journals Selective inhibition of CD4+ T-cell cytokine production and autoimmunity by BET protein and c-Myc inhibitors

2012 ◽  
Vol 109 (36) ◽  
pp. 14532-14537 ◽  
Author(s):  
H. S. Bandukwala ◽  
J. Gagnon ◽  
S. Togher ◽  
J. A. Greenbaum ◽  
E. D. Lamperti ◽  
...  
Keyword(s):  
T Cell ◽  
Cytokine Production ◽  
Selective Inhibition ◽  
Cd4 T Cell ◽  
Bet Protein ◽  
Cell Cytokine Production
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