Effects of oral administration of collagen peptides on skin collagen content and its underlying mechanism using a newly developed low collagen skin mice model

2015 ◽  
Vol 16 ◽  
pp. 174-182 ◽  
Author(s):  
Akinori Haratake ◽  
Daisuke Watase ◽  
Takuya Fujita ◽  
Shuichi Setoguchi ◽  
Kazuhisa Matsunaga ◽  
...  
Keyword(s):  
Oral Administration ◽  
Underlying Mechanism ◽  
Collagen Content ◽  
Mice Model ◽  
Collagen Peptides ◽  
Skin Collagen

Metabolomics strategy reveals the osteogenic mechanism of yak (Bos grunniens) bone collagen peptides on ovariectomy-induced osteoporosis in rats

2020 ◽  
Vol 11 (2) ◽  
pp. 1498-1512 ◽  
Author(s):  
Mengliang Ye ◽  
Chunhui Zhang ◽  
Wei Jia ◽  
Qingshan Shen ◽  
Xiaojie Qin ◽  
...  
Keyword(s):  
Underlying Mechanism ◽  
Bone Collagen ◽  
Targeted Metabolomics ◽  
First Report ◽  
Bos Grunniens ◽  
Collagen Peptides ◽  
Positive Effect

This is the first report on the positive effect and underlying mechanism of yak bone collagen peptides in the treatment of osteoporotic rats based on non-targeted metabolomics.

scholarly journals Altered Fecal Metabolites and Colonic Glycerophospholipids Were Associated With Abnormal Composition of Gut Microbiota in a Depression Model of Mice

2021 ◽  
Vol 15 ◽  
Author(s):  
Xue Gong ◽  
Cheng Huang ◽  
Xun Yang ◽  
Jianjun Chen ◽  
Juncai Pu ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Underlying Mechanism ◽  
Rrna Gene ◽  
Mice Model ◽  
Microbial Composition ◽  
Gas And Liquid Chromatography ◽  
Correlation Analysis Method ◽  
Rrna Gene Sequencing

The microbiota–gut–brain axis has been considered to play an important role in the development of depression, but the underlying mechanism remains unclear. The gastrointestinal tract is home to trillions of microbiota and the colon is considered an important site for the interaction between microbiota and host, but few studies have been conducted to evaluate the alterations in the colon. Accordingly, in this study, we established a chronic social defeated stress (CSDS) mice model of depression. We applied 16S rRNA gene sequencing to assess the gut microbial composition and gas and liquid chromatography–mass spectroscopy to identify fecal metabolites and colonic lipids, respectively. Meanwhile, we used Spearman’s correlation analysis method to evaluate the associations between the gut microbiota, fecal metabolites, colonic lipids, and behavioral index. In total, there were 20 bacterial taxa and 18 bacterial taxa significantly increased and decreased, respectively, in the CSDS mice. Further, microbial functional prediction demonstrated a disturbance of lipid, carbohydrate, and amino acid metabolism in the CSDS mice. We also found 20 differential fecal metabolites and 36 differential colonic lipids (in the category of glycerolipids, glycerophospholipids, and sphingolipids) in the CSDS mice. Moreover, correlation analysis showed that fecal metabolomic signature was associated with the alterations in the gut microbiota composition and colonic lipidomic profile. Of note, three lipids [PC(16:0/20:4), PG(22:6/22:6), and PI(18:0/20:3), all in the category of glycerophospholipids] were significantly associated with anxiety- and depression-like phenotypes in mice. Taken together, our results indicated that the gut microbiota might be involved in the pathogenesis of depression via influencing fecal metabolites and colonic glycerophospholipid metabolism.

scholarly journals Bioactivities of Stixis suaveolens (Roxb.) Fruit Extract: An Evaluation in Mice Model

2020 ◽  
Vol 23 (2) ◽  
pp. 135-140
Author(s):  
Md Monirul Islam ◽  
Tufael Ahmed ◽  
Hajera Khatun ◽  
Mohammad A Rashid
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Oral Intake ◽  
Tail Flick ◽  
Mice Model ◽  
Standard Drug ◽  
Isolation And Characterization

The fruits of Stixis suaveolens (Roxb.) have been a popular folk medicine among traditional practitioners. However, there are questions about its traditional uses due to lack of scientific evidence. This study was aimed to evaluate the effects of crude methanol extract of fruits of S. suaveolens in mice model. The central and peripheral analgesic activity were evaluated using the ‘tail flick’ and ‘writhing’ assay respectively. The anti-hyperglycemic potential was assessed by the ability of the crude extract in reducing blood glucose level in mice after oral administration of glucose. Oral administration (400 mg/kg bw) of the extract showed significant (p<0.001) delay in pain sensation and inhibition of acetic acid induced writhing response in mice model. The results were compared with the respective standard morphine (2 mg/kg bw) and diclofenac (50 mg/kg bw). Likewise, in anti-hyperglycemic assay, maximum reduction (p<0.001) of blood glucose level (39.6%) was observed 120 min after oral intake (400 mg/kg bw) of the extract as compared that exhibited by the standard drug, glibenclamide (46.83%). The in vivo bioassays confirmed that the crude methanolic extract of fruits of S. suaveolens possesses significant central- and peripheral-analgesic as well as anti-hyperglycemic activities. These findings justify its popularity as a traditional medicine and hence demands future study involving isolation and characterization of its bioactive compounds. Bangladesh Pharmaceutical Journal 23(2): 135-140, 2020

The effects of dietary nitrogen level on the collagen of rat skin

1978 ◽  
Vol 39 (1) ◽  
pp. 181-192 ◽  
Author(s):  
R. Dawson ◽  
G. Milne
Keyword(s):  
Crude Protein ◽  
Collagen Content ◽  
Male Rats ◽  
Control Group ◽  
Protein Nitrogen ◽  
Total N ◽  
Dietary Nitrogen ◽  
Low Protein ◽  
Skin Collagen ◽  
Commercial Stock

1. Male rats of approximately 120 g body-weight were maintained on a commercial stock diet containing 204 g crude protein (nitrogen × 6.25)/kg, a hydroxyproline-free high-protein (HP) diet containing 200 g casein/kg as the only protein source, or a low-protein (LP) diet containing 40 g casein/kg. After 6 weeks on these diets half of each group was transferred to a non-protein (NP) diet and the experiment was continued for a further 6 weeks. Animals from each group were killed at 4 d, 3 weeks and 6 weeks after the transfer to the NP diet.2. Throughout the experiment the urinary excretion of N, hydroxyproline and creatinine, and the content and solubility of the skin collagen were determined.3. When compared with a control group killed at the beginning of the experiment the rats maintained on the LP diet showed an increase of 25% in total N content of the skin but collagen content increased by 100%. Rats transferred from the HP to the NP diet lost both N and collagen from the skin, but those transferred from the LP to the NP diet lost N but increased the collagen content by 42%.4. Protein deprivation brought about marked changes in the solubility of the skin collagen, suggesting an increase in the rate of maturation of skin collagen.

scholarly journals Effect of Oral Ingestion of Low-Molecular Collagen Peptides Derived from Skate (Raja Kenojei) Skin on Body Fat in Overweight Adults: A Randomized, Double-Blind, Placebo-Controlled Trial

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 157 ◽  
Author(s):  
Young Tak ◽  
Yun Kim ◽  
Jeong Lee ◽  
Yu-Hyun Yi ◽  
Young Cho ◽  
...  
Keyword(s):  
Body Fat ◽  
Animal Studies ◽  
Controlled Trial ◽  
Intervention Group ◽  
Control Group ◽  
Double Blind ◽  
Collagen Peptides ◽  
Collagen Peptide ◽  
Skin Collagen ◽  
Overweight Adults

Recent animal studies found the potential of a collagen peptide derived from skate skin to have anti-obesity effects through the suppression of fat accumulation and regulation of lipid metabolism. However, no studies have yet been performed in humans. Here, this very first human randomized, placebo-controlled, and double-blinded study was designed to investigate the efficacy and tolerability of skate skin collagen peptides (SCP) for the reduction of body fat in overweight adults. Ninety healthy volunteers (17 men) aged 41.2 ± 10.4 years with a mean body mass index of 25.6 ± 1.9 kg/m2 were assigned to the intervention group (IG), which received 2000 mg of SCP per day or to the control group (CG) given the placebo for 12 weeks and 81 (90%) participants completed the study. Changes in body fat were evaluated using dual energy X-ray absorptiometry as a primary efficacy endpoint. After 12 weeks of the trial, the percentage of body fat and body fat mass (kg) in IG were found to be significantly better than those of subjects in CG (−1.2% vs. 2.7%, p = 0.024 and −1.2 kg vs. 0.3 kg, p = 0.025). Application of SCP was well tolerated and no notable adverse effect was reported from both groups. These results suggest the beneficial potential of SCP in the reduction of body fat in overweight adults.

Modulation and the Underlying Mechanism of T Cells in Thymus of Mice by Oral Administration of Sodium Fluoride

2015 ◽  
Vol 170 (1) ◽  
pp. 194-200 ◽  
Author(s):  
Songna Yin ◽  
Haibo Wu ◽  
Chao Song ◽  
Xin Chen ◽  
Yong Zhang
Keyword(s):  
T Cells ◽  
Oral Administration ◽  
Sodium Fluoride ◽  
Underlying Mechanism

scholarly journals Involvement of the Cerebral Monoamine Neurotransmitters System in Antidepressant-Like Effects of a Chinese Herbal Decoction, Baihe Dihuang Tang, in Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Meng-Li Chen ◽  
Jie Gao ◽  
Xin-Rong He ◽  
Qian Chen
Keyword(s):  
Depressive Disorders ◽  
Forced Swim Test ◽  
Tail Suspension Test ◽  
Underlying Mechanism ◽  
Mice Model ◽  
Monoamine Neurotransmitters ◽  
Chinese Herbal ◽  
Thought Disorders ◽  
Immobility Time ◽  
Monoaminergic Neurotransmitter

Baihe Dihuang Tang (BDT) is a renowned Chinese herbal formula which is commonly used for treating patients with mental instability, absentmindedness, insomnia, deficient dysphoria, and other psychological diseases. These major symptoms closely associated with the depressive disorders. BDT was widely popular use for treating emotion-thought disorders for many years in China. In the present study, the antidepressant-like effect of BDT in mice was investigated by using the forced swim test (FST) and the tail suspension test (TST). The underlying mechanism was explored by determining the effect of BDT on the level of cerebral monoamine neurotransmitters. BDT (9 and 18 g/kg, p.o. for 14 days) administration significantly reduced the immobility time in both the FST and the TST without changing locomotion in the open field-test (OFT). Moreover, BDT treatment at the dose of 18 g/kg inhibited reserpine-induced ptosis. Meanwhile, BDT enhanced 5-HT and NA levels in mouse cerebrum as well as decreased the ratio of 5-HT compared to its metabolite, 5-HIAA, (turnover, 5-HIAA/5-HT) after TST. The results demonstrated that the antidepressant-like effect of BDT is mediated, at least partially, via the central monoaminergic neurotransmitter system.

scholarly journals Histamine augments collagen content via H1 receptor stimulation in cultures of myofibroblasts taken from wound granulation tissue

2020 ◽  
Author(s):  
Monika Wolak ◽  
Ewa Bojanowska ◽  
Teresa Staszewska ◽  
Lucyna Piera ◽  
Jacek Szymański ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Smooth Muscle Actin ◽  
Histamine Receptor ◽  
Collagen Content ◽  
Type I ◽  
H1 Receptor ◽  
Alpha Smooth Muscle Actin ◽  
Receptor Stimulation ◽  
Experimental Conditions ◽  
Skin Collagen

AbstractThe inflammatory reaction influences the deposition of collagen within wound granulation tissue. The aim of the present study is to determine whether histamine acting directly on myofibroblasts derived from wound granulation tissue may influence collagen deposition. It also identifies the histamine receptor involved in this process. The experiments were carried out on cells isolated from the granulation tissue of a wound model (a polypropylene net inserted subcutaneously to rats) or intact rat skin. Collagen content was measured following the addition of different concentrations of histamine and treatment with histamine receptor antagonists (ketotifen – H1 inhibitor, ranitidine – H2 inhibitor) and a histamine receptor H1 agonist (2-pyridylethylamine dihydrochloride).The cells were identified as myofibroblasts: alpha-smooth muscle actin, vimentin, and desmin positive in all experimental conditions. Histamine increased the collagen level within both cell cultures, i.e., those isolated from granulation tissue or intact skin. It did not, however, influence the expression of either the collagen type I or III genes within the cultured myofibroblasts. Histamine activity was reduced by ketotifen (the H1 receptor inhibitor) and increased by the H1 receptor agonist, as demonstrated by changes in the levels of collagen in the myofibroblast culture. Histamine increased collagen content within the cultures, acting directly on myofibroblasts via H1 receptor stimulation.

Influence of oral administration of Akkermansia muciniphila on the tissue distribution and gut microbiota composition of acute and chronic cadmium exposure mice

2019 ◽  
Vol 366 (13) ◽  
Author(s):  
Saisai Feng ◽  
Yang Liu ◽  
Yanyang Huang ◽  
Jianxin Zhao ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Oral Administration ◽  
Graphite Furnace ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Mice Model ◽  
Akkermansia Muciniphila ◽  
Gut Barrier Function ◽  
Cd Exposure ◽  
Gut Microbiota Composition

ABSTRACT Cadmium (Cd) contamination is a serious food safety problem. Acute and chronic Cd exposure changes the gut microbiota composition and damages the gut barrier function. Akkermansia muciniphila (AKK), a promising candidate for the next-generation probiotics, has been reported to protect the mucus layer in the colon and significantly decrease the effects of Cd exposure in mice. Thus, the mice model was adopted to investigate the influence of oral administration of AKK on the toxic distribution and changes of gut microbiota composition caused by acute and chronic Cd exposure. In both acute and chronic Cd exposure experiments, 40 mice were divided into four groups (normal group, AKK group, Cd group and Cd plus AKK group). The Cd contents in feces and tissues were measured by a flame or graphite furnace atomic absorption spectrophotometer and gut microbiota composition was determined through 16S rRNA gene sequencing. The results showed that the gavage of AKK could not reduce the accumulation of Cd in the liver and kidney. The oral administration of AKK showed a certain influence on the gut microbiota composition of acute Cd exposure mice and limited influence on that of chronic Cd exposure mice. These results indicate the failure of AKK, as a potential protective probiotic, to reduce Cd toxicity. However, the gavage of AKK did have an influence on the gut microbiota composition of normal mice, especially on some genera in the Clostridiales order. Besides, when considering AKK’s probiotic potential and its effects on host health and disease, we should take into consideration its influence on the gut microbiota composition and micro-environment.

scholarly journals Sex hormones and skin collagen content in postmenopausal women.

BMJ ◽  
1983 ◽  
Vol 287 (6402) ◽  
pp. 1337-1338 ◽  
Author(s):  
M Brincat ◽  
C F Moniz ◽  
J W Studd ◽  
A J Darby ◽  
A Magos ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Sex Hormones ◽  
Collagen Content ◽  
Skin Collagen
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