Tangeretin, a polymethoxylated flavone, modulates lipid homeostasis and decreases oxidative stress by inhibiting NF-κB activation and proinflammatory cytokines in cardiac tissue of streptozotocin-induced diabetic rats

2015 ◽  
Vol 16 ◽  
pp. 315-333 ◽  
Author(s):  
Ramalingam Sundaram ◽  
Palanivelu Shanthi ◽  
Panchanatham Sachdanandam
Keyword(s):  
Oxidative Stress ◽  
Proinflammatory Cytokines ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Lipid Homeostasis

scholarly journals Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 335
Author(s):  
Estefanía Bravo-Sánchez ◽  
Donovan Peña-Montes ◽  
Sarai Sánchez-Duarte ◽  
Alfredo Saavedra-Molina ◽  
Elizabeth Sánchez-Duarte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Sensitivity ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Oxidase Inhibitor ◽  
Beneficial Effects ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Transport Chain ◽  
Male Wistar Rats

Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.

scholarly journals Preventive effects of garlic (Allium sativum) on oxidative stress and histopathology of cardiac tissue in streptozotocin-induced diabetic rats

2015 ◽  
Vol 102 (4) ◽  
pp. 380-390 ◽  
Author(s):  
R Naderi ◽  
G Mohaddes ◽  
M Mohammadi ◽  
A Alihemmati ◽  
R Badalzadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Allium Sativum ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Preventive Effects

Does Short-Term and Low-Dose N-Acetylcysteine Affect Oxidative Stress and Inflammation in The Liver Tissues of Diabetic Rats?

2021 ◽  
pp. 109980042110036
Author(s):  
Fatma Genç ◽  
Emine Gülçeri GÜLEÇ Peker
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Proinflammatory Cytokines ◽  
Liver Tissue ◽  
Low Dose ◽  
Biochemical Parameter ◽  
Diabetic Rats ◽  
Parameter Analysis ◽  
Albino Rats ◽  
Short Term

Diabetes mellitus is a serious chronic disease in which the oxidant-antioxidant balance is impaired, causing many complications, including hepatopathy. In this study, the effects of short-term and low-dose N-acetylcysteine (NAC) administration on the biochemical, proinflammatory, and oxidative stress parameters in the liver tissue of diabetic rats were investigated. Twenty-four adult male Wistar albino rats weighing approximately 250–300 g were divided into 4 groups (n = 6): Control, Streptozotosin (STZ)-induced diabetes (DM), NAC treatment (60 mg/kg), and STZ-induced diabetes treated with NAC (DM+NAC; 60 mg/kg). NAC treatment was administered intraperitoneally as a single daily dose for 7 days. At the end of the experiment (3 weeks), blood and liver samples were collected for biochemical parameter analysis. Lipid peroxidation, antioxidant parameters, and nitric oxide (NOx) levels were determined by spectrophotometric method. Tissue inflammation parameters were evaluated by ELISA. Lipid peroxidation, proinflammatory cytokines, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values increased significantly with diabetes. NAC treatment significantly decreased serum ALT and AST levels and proinflammatory cytokines in the diabetic group. Liver glutathione (GSH) and NOx levels increased significantly in the DM+NAC group ( p < 0.05). While NAC treatment reduced lipid peroxidation in the liver, it improved the inflammatory response and antioxidant status. The beneficial effect of NAC treatment may be due to its antioxidant activity and the resulting increased level of GSH. The results show that low-dose and short-term NAC treatment had a positive effect on oxidative damage and inflammation in liver tissue. NAC can be used as a potential antioxidant in diabetes to prevent hepatopathy.

Enalapril (E) Attenuates Oxidative Stress (OXS) in Diabetic Rats.

Hypertension ◽  
2000 ◽  
Vol 36 (suppl_1) ◽  
pp. 726-726
Author(s):  
Elena MV de Cavanagh ◽  
Cesar G Fraga ◽  
Jorge E Toblli ◽  
Felipe Inserra ◽  
Leon F Ferder
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Cardiac Tissue ◽  
Tissue Injury ◽  
Tap Water ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Total Glutathione ◽  
Tubulointerstitial Lesions ◽  
And Control

P183 Oxidative stress has been involved as a possible mechanism of tissue injury. The effect of E on OxS and tissue injury was studied in kidney and heart from streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley, were divided into: STZ (65 mg/kg, single i.p. dose); STZ+E (STZ and E, 20 mg E /L drinking water), and Control (C) (saline i.p. and tap water). At 6 mo. organs were studied by light microscopy. Glomerular (GML) and tubulointerstitial lesions (TIL) were graded by a semiquantitative score (0-4). Glycemia, creatinine clearance (CrCl) and proteinuria were determined. OxS was evaluated by determining total glutathione content (GSH), protein-associated sulfhydryls (SH) and 2-thiobarbituric reactive substances (TBARS, an indicator of lipid oxidation) in kidney and heart homogenates. In STZ rats E decreased proteinuria, GML and TIL,and increased CrCl. In kidney and heart, E attenuated OxS associated to STZ. Results suggest that, in addition to previously described mechanisms, E might protect renal and cardiac tissue by attenuating OxS in STZ-induced diabetic rats.

scholarly journals Attenuation of oxidative stress and cardioprotective effects of zinc supplementation in experimental diabetic rats

2017 ◽  
Vol 117 (3) ◽  
pp. 335-350 ◽  
Author(s):  
Susmita Barman ◽  
Krishnapura Srinivasan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Hepatic Tissue ◽  
Antioxidant Enzyme Activities ◽  
Markers Of Oxidative Stress ◽  
Cardioprotective Effects ◽  
Apoptotic Factors

AbstractOxidative stress plays a major role in the pathogenesis of diabetes mellitus, which further exacerbates damage of cardiac, hepatic and other tissues. We have recently reported that Zn supplementation beneficially modulates hyperglycaemia and hypoinsulinaemia, with attendant reduction of associated metabolic abnormalities in diabetic rats. The present study assessed the potential of Zn supplementation in modulating oxidative stress and cardioprotective effects in diabetic rats. Diabetes was induced in Wistar rats with streptozotocin, and groups of diabetic rats were treated with 5- and 10-fold dietary Zn interventions (0·19 and 0·38 g Zn/kg diet) for 6 weeks. The markers of oxidative stress, antioxidant enzyme activities and concentrations of antioxidant molecules, lipid profile, and expressions of fibrosis and pro-apoptotic factors in the cardiac tissue were particularly assessed. Supplemental Zn showed significant attenuation of diabetes-induced oxidative stress in terms of altered antioxidant enzyme activities and increased the concentrations of antioxidant molecules. Hypercholesterolaemia and hyperlipidaemia were also significantly countered by Zn supplementation. Along with attenuated oxidative stress, Zn supplementation also showed significant cardioprotective effects by altering the mRNA expressions of fibrosis and pro-apoptotic factors (by >50 %). The expression of lipid oxidative marker 4-hydroxy-2-nonenal (4-HNE) protein in cardiac tissue of diabetic animals was rectified (68 %) by Zn supplementation. Elevated cardiac and hepatic markers in circulation and pathological abnormalities in cardiac and hepatic tissue architecture of diabetic animals were ameliorated by dietary Zn intervention. The present study indicates that Zn supplementation can attenuate diabetes-induced oxidative stress in circulation as well as in cardiac and hepatic tissues.

scholarly journals Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 579 ◽  
Author(s):  
Maria Zych ◽  
Weronika Wojnar ◽  
Sławomir Borymski ◽  
Katarzyna Szałabska ◽  
Piotr Bramora ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Cardiac Tissue ◽  
Sinapic Acid ◽  
Diabetic Rats ◽  
Female Rats ◽  
Oxidative Stress Parameters ◽  
Type 2 Diabetic ◽  
Stress Parameters

Cardiovascular diseases are one of the most common complications of type 2 diabetes. They are considered the leading cause of death among diabetics. One of the mechanisms underlying diabetic cardiovascular complications is oxidative stress. Many phenolic acids are regarded as antioxidants. The aim of the study was to investigate the effect of rosmarinic acid (RA) and sinapic acid (SA) on oxidative stress parameters in the cardiac tissue and serum of type 2 diabetic female rats. Additionally, the effect of these compounds on glucose homeostasis and lipid profile in the serum was evaluated. Type 2 diabetes was induced with high-fat diet and streptozotocin. RA at the doses of 10 and 50 mg/kg and SA at the doses of 5 and 25 mg/kg were administrated orally for 28 days. Untreated diabetic rats exhibited unfavorable changes in glucose metabolism and lipid profile. Changes in the enzymatic and non-enzymatic markers indicated the onset of oxidative stress in these animals. The results showed that the higher doses of the tested phenolic acids—50 mg/kg of RA and 25 mg/kg of SA—revealed beneficial effects on oxidative stress in the cardiac tissue of diabetic rats.

Antioxidant and anti-inflammatory effect of olive leaf extract treatment in diabetic rat brain

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mehmet Berköz ◽  
Tahir Kahraman ◽  
Zwain Nozad Shamsulddin ◽  
Mirosław Krośniak
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Proinflammatory Cytokines ◽  
Leaf Extract ◽  
Glycated Hemoglobin ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Olive Leaf ◽  
Olive Leaf Extract

Abstract Objectives Olive (Olea europaea L.) plays a promising role in pharmaceutical, nutraceutical, and cosmetic production. On the other hand, olive leaf is widely used in folk medicine due to its antihyperglycemic activity. For this aim, possible effects of olive leaf extract (OLE) in the brain tissue of streptozotocin-induced diabetic rats were investigated. Methods A total of 28 male rats were divided into four equal groups as control, diabetic (single dose of 45 mg/kg streptozotocin, i.p.), OLE (500 mg/kg/day), and diabetic + OLE groups. The study was terminated 21 days after the diabetes model was formed. At the end of the study, all the animals were sacrificed and blood and brain tissues were isolated. Relative brain weights, complete blood count, blood glycated hemoglobin, serum glucose, total protein, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, insulin, gonadal hormone levels, production and messenger ribonucleic acid (mRNA) levels of proinflammatory cytokines and mediators, total thiol, total oxidative stress, and total antioxidant status levels and fatty acid composition in brain tissue were measured in all study groups. Results In diabetic rats, relative brain weight and serum insulin level decreased, glycated hemoglobin, oxidative stress, production and mRNA level of proinflammatory cytokines and mediators increased, hyperglycemia, hypercholesterolemia and hypertriglyceridemia, degraded fatty acid composition, anemia, leukopenia, and thrombocytopenia occurred. After OLE treatment, a remarkable improvement in most of these parameters, except gonadal hormones, has been observed in diabetic rats. Conclusions This study suggests that olive leaf can be a precious neuroprotective agent in diabetes.

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