scholarly journals Potential mechanism of mycelium polysaccharide from Pholiota dinghuensis Bi in regulating the proliferation and apoptosis of human breast cancer MCF-7 cells through p38/MAPK pathway

2015 ◽  
Vol 12 ◽  
pp. 375-388 ◽  
Author(s):  
Dan Gan ◽  
Xiaoxiong Zeng ◽  
Rui Hai Liu ◽  
Hong Ye
Keyword(s):  
Breast Cancer ◽  
P38 Mapk ◽  
Human Breast Cancer ◽  
Mapk Pathway ◽  
Potential Mechanism ◽  
Human Breast ◽  
P38 Mapk Pathway ◽  
Proliferation And Apoptosis ◽  
Mcf 7

scholarly journals The combination of baicalin and baicalein enhances apoptosis via the ERK/p38 MAPK pathway in human breast cancer cells

2009 ◽  
Vol 30 (12) ◽  
pp. 1648-1658 ◽  
Author(s):  
Qian-mei Zhou ◽  
Song Wang ◽  
Hui Zhang ◽  
Yi-yu Lu ◽  
Xiu-feng Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
P38 Mapk ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Mapk Pathway ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
P38 Mapk Pathway

scholarly journals In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

2016 ◽  
Vol 40 (3-4) ◽  
pp. 807-817 ◽  
Author(s):  
Hong-Yan Zhang ◽  
Feng Liang ◽  
Fei Wang ◽  
Jian-Wei Zhang ◽  
Li Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Proliferation And Apoptosis ◽  
Sirna Group ◽  
Number Of Cells ◽  
Mcf 7

Background: Breast cancer is characterized by a distinct metastatic pattern involving the regional lymph nodes, bone marrow, lung and liver. This study is aimed to investigate the effects of silencing the HAS-2 gene on the proliferation and apoptosis of human breast cancer cells. Methods: MCF-7 cells were collected and assigned into control, scrambled siRNA and HAS-2- siRNA groups. After transfection, the morphological changes in the MCF-7 cells were observed using phase contrast microscopy. qRT-PCR and Western blot assays were used to detect the mRNA and protein expression of apoptosis-related proteins. CCK-8 and flow cytometry were performed to evaluate cell proliferation, the cell cycle and apoptosis. Results: In the control and the scrambled siRNA groups, cells grew adhered to the wall and mainly showed a spindle shape with a clear nucleolus. Compared with the control and scrambled siRNA groups, increases in the number of cells in early apoptosis and metaphase cells in apoptosis were observed in the HAS-2-siRNA group. The HAS-2-siRNA group showed decreased expression of HAS-2 relative to that in the control and scrambled siRNA groups. No significant differences in cell proliferation, cell cycle distribution or apoptosis were noted between the control and scrambled siRNA groups. In the HAS-2-siRNA group, the cell proliferation ability decreased significantly, but the number of cells in the G0/G1 stage, the number of apoptotic cells and the expression of caspase-3 and caspase-9 increased significantly. Conclusion: Our findings indicate that HAS-2 gene silencing may inhibit proliferation and promote apoptosis in the MCF-7 human breast cancer cell line.

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