Influence of traditionally used Nepalese plants on wound healing and immunological properties using primary human cells in vitro

2019 ◽  
Vol 235 ◽  
pp. 415-423 ◽  
Author(s):  
Amy M. Zimmermann-Klemd ◽  
Viktoria Konradi ◽  
Carmen Steinborn ◽  
Annekathrin Ücker ◽  
Chiara Madlen Falanga ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Cells ◽  
Immunological Properties ◽  
Primary Human Cells

scholarly journals A CellAgeClock for expedited discovery of anti-ageing compounds

2019 ◽  
Author(s):  
Celia Lujan ◽  
Eleanor J. Tyler ◽  
Simone Ecker ◽  
Amy P. Webster ◽  
Eleanor R. Stead ◽  
...  
Keyword(s):  
Cpg Methylation ◽  
Human Cells ◽  
Epigenetic Clock ◽  
The Novel ◽  
Drug Treatments ◽  
Screening Platform ◽  
Methylation Profiling ◽  
Primary Human Cells

AbstractWe aim to improve anti-ageing drug discovery, currently achieved through laborious and lengthy longevity analysis. Recent studies demonstrated that the most accurate molecular method to measure human age is based on CpG methylation profiles, as exemplified by several epigenetics clocks that can accurately predict an individual’s age. Here, we developed CellAgeClock, a new epigenetic clock that measures subtle ageing changes in primary human cells in vitro. As such, it provides a unique tool to measure effects of relatively short pharmacological treatments on ageing. We validated the CellAgeClock against known longevity drugs such as rapamycin and trametinib. Moreover, we uncovered novel anti-ageing drugs, torin2 and Dactolisib (BEZ-235), demonstrating the value of our approach as a screening and discovery platform for anti-ageing strategies. The CellAgeClock outperforms other epigenetic clocks in measuring subtle ageing changes in primary human cells in culture. The tested drug treatments reduced senescence and other ageing markers, further consolidating our approach as a screening platform. Finally, we show that the novel anti-ageing drugs we uncovered in vitro, indeed increased longevity in vivo. Our method expands the scope of CpG methylation profiling from measuring human chronological and biological age from human samples in years, to accurately and rapidly detecting anti-ageing potential of drugs using human cells in vitro, providing a novel accelerated discovery platform to test sought after geroprotectors.

scholarly journals Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

2019 ◽  
Vol 4 (1) ◽  
pp. e000217 ◽  
Author(s):  
Yoshimi Niwano ◽  
Atsuo Iwasawa ◽  
Kazuo Tsubota ◽  
Masahiko Ayaki ◽  
Kazuno Negishi
Keyword(s):  
Blue Light ◽  
Ocular Surface ◽  
Human Cells ◽  
Light Irradiation ◽  
Protective Effects ◽  
Corneal Surface ◽  
Surface Protection ◽  
Viable Cells ◽  
Primary Human Cells

ObjectiveBlue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin.Methods and analysisPrimary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours’ incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades.ResultsUnder subconfluent conditions, viable cells decreased by around 50% after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30%–40% reduction (ultraviolet) and 15%–30% reduction (blue light) in viable cells.ConclusionThese results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.

scholarly journals Neutrophil activation by nanomaterials in vitro: comparing strengths and limitations of primary human cells with those of an immortalized (HL-60) cell line

2020 ◽  
pp. 1-20
Author(s):  
Rachel Verdon ◽  
Suzanne L. Gillies ◽  
David M. Brown ◽  
Theodore Henry ◽  
Lang Tran ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cells ◽  
Neutrophil Activation ◽  
Primary Human Cells

scholarly journals Mitotic non-disjunction as a mechanism for in vitro aneuploidy induction by X-rays in primary human cells

Mutagenesis ◽  
1996 ◽  
Vol 11 (4) ◽  
pp. 307-313 ◽  
Author(s):  
M. Kirsch-Volders ◽  
I. Tallon ◽  
C. Tanzarella ◽  
A. Sgura ◽  
T. Hermine ◽  
...  
Keyword(s):  
Human Cells ◽  
X Rays ◽  
Primary Human Cells

scholarly journals Neutrophil trafficking on-a-chip: an in vitro, organotypic model for investigating neutrophil priming, extravasation, and migration with spatiotemporal control

Lab on a Chip ◽  
2019 ◽  
Vol 19 (21) ◽  
pp. 3697-3705 ◽  
Author(s):  
Patrick H. McMinn ◽  
Laurel E. Hind ◽  
Anna Huttenlocher ◽  
David J. Beebe
Keyword(s):  
Human Cells ◽  
Microfluidic Technology ◽  
And Migration ◽  
High Degree ◽  
Spatiotemporal Control ◽  
Neutrophil Priming ◽  
Primary Human Cells

Her we report a new microfluidic technology designed to facilitate the study of neutrophil trafficking and priming using primary human cells with a high degree of spatiotemporal control.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

In vitro anti-inflammatory and wound healing activities of Citrus auraptene

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
F Epifano ◽  
S Genovese ◽  
L Zhao ◽  
V Dang La ◽  
D Grenier
Keyword(s):  
Wound Healing ◽  
Anti Inflammatory
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