Anti-inflammatory activity of Eupatorium perfoliatum L. extracts, eupafolin, and dimeric guaianolide via iNOS inhibitory activity and modulation of inflammation-related cytokines and chemokines

2011 ◽  
Vol 137 (1) ◽  
pp. 371-381 ◽  
Author(s):  
Mareike Maas ◽  
Alexandra M. Deters ◽  
Andreas Hensel
Keyword(s):  
Inhibitory Activity ◽  
Inflammatory Activity ◽  
Cytokines And Chemokines ◽  
Anti Inflammatory ◽  
Eupatorium Perfoliatum

Advances on the Anti-inflammatory Activity of Oleanolic Acid and Derivatives

2021 ◽  
Vol 21 ◽  
Author(s):  
Xiaofan Zhang ◽  
Huiyun Wang ◽  
Yangrong Xu ◽  
Mingzhu Luan ◽  
Fenglan Zhao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Signaling Pathways ◽  
Oleanolic Acid ◽  
Inhibitory Activity ◽  
Research Work ◽  
Inflammatory Activity ◽  
Acetate Ester ◽  
Stat Signaling ◽  
Anti Inflammatory ◽  
Cortical System

: Oleanolic acid can inhibit edema and exhibit obvious inhibitory activity to inflammatory by activating of the pituitary-adrenal cortical system, inhibiting the synthesis or release of PGs, inhibiting endotoxin-mediated release of HMGB1 by endothelial cells or regulating MAPK, PI3K/Akt/NF-κB/ICAM-1/JAK/STAT signaling pathways, etc. In recent years, an increased number of interesting research work has been carried out on the anti-inflammatory activity and mechanisms of OA derivatives, such as acyloxyimino derivative, 3-acetylated derivatives, novel 3,5-disubstituted isoxazoles derivatives, acetate, ester derivatives and oximes derivatives. The review summaries and highlights the update advances on the anti-inflammatory activity and mechanism of OA and its derivatives.

Antrolone, a Novel Benzoid Derived from Antrodia cinnamomea, Inhibits the LPS-Induced Inflammatory Response in RAW264.7 Macrophage Cells by Balancing the NF-κB and Nrf2 Pathways

2018 ◽  
Vol 46 (06) ◽  
pp. 1297-1313 ◽  
Author(s):  
I-Chuan Yen ◽  
Li-Shian Shi ◽  
Min-Chieh Chung ◽  
Blerina Ahmetaj-Shala ◽  
Tsu-Chung Chang ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Heme Oxygenase 1 ◽  
Prostaglandin E ◽  
Related Factor ◽  
Antrodia Cinnamomea ◽  
Cytokines And Chemokines ◽  
Anti Inflammatory ◽  
Underlying Mechanisms ◽  
Raw264.7 Macrophage ◽  
Pro Inflammatory Cytokines

Antrodia cinnamomea, a medicinal mushroom, has previously demonstrated anti-inflammatory activity, although the specific compound responsible for the effect remains unclear. The present study was designed to investigate the anti-inflammatory property of antrolone, a novel benzoid derived from A. cinnamomea mycelium, and to clarify the underlying mechanisms of action. To this end, murine macrophage RAW264.7 cells were treated with antrolone (0.1–30[Formula: see text][Formula: see text]M) 30[Formula: see text]min prior to stimulation with lipopolysaccharides (LPS, 0.1[Formula: see text][Formula: see text]g/ml) for 24[Formula: see text]h. Cell viability, nitric oxide (NO) and prostaglandin E2 (PGE2) production, levels of pro-inflammatory cytokines and chemokines, and the signaling pathways involved in the inflammatory cascades were then investigated. Our results show that antrolone significantly decreased LPS-induced NO, PGE2, pro-inflammatory cytokine, and keratinocyte chemoattractant CXCL1 (KC) production and reduced levels of the proteins inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). These effects were independent of the effect of antrolone on macrophage cytotoxicity. Moreover, antrolone significantly inhibited the activation of the NF[Formula: see text]B, MAPK, and AKT pathways, while it increased nuclear factor erythroid-2-related factor (Nrf2) and heme oxygenase-1 (HO-1) levels. Our findings suggest that antrolone exhibits potent anti-inflammatory activity and may, therefore, be a lead compound for the development of an anti-inflammatory drug.

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF 4-BENZYLPIPERIDINE

2016 ◽  
pp. 108 ◽  
Author(s):  
Jayashree V ◽  
Bagyalakshmi S ◽  
Manjula Devi K ◽  
Richard Daniel D
Keyword(s):  
Inhibitory Activity ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Significant Activity ◽  
Standard Drug ◽  
Study Results ◽  
Anti Inflammatory ◽  
Dose Dependent

<p>ABSTRACT<br />Objective: To study the in vitro anti-inflammatory activity of 4-benzylpiperidine.<br />Methods: This study was conducted to evaluate the in vitro anti-inflammatory activity of 4-benzylpiperidine using in vitro models such as inhibition<br />of albumin denaturation and proteinase inhibitory activity.<br />Results: This study revealed the dose-dependent inhibition of protein denaturation and proteinase inhibitory activity by 4-benzylpiperidine.<br />Conclusion: In the present study, results indicate that the 4-benzylpiperidine possess anti-inflammatory properties. The drug inhibited the heat<br />induced albumin denaturation and proteinase inhibitory activity. It shows dose-dependent significant activity when compared with a standard drug.<br />Hence, this study gives an idea that the 4-benzylpiperidine can be used as a lead compound for designing a potent anti-inflammatory drug which can<br />be used to cure inflammation.<br />Keywords: Anti-inflammatory activity, 4-Benzylpiperidine, Protein denaturation, Proteinase inhibitory activity.</p>

scholarly journals Formulation and Evaluation of Poly Herbal Liqui-Solid Compact for its Anti-Inflammatory Effect

2021 ◽  
Vol 12 (3) ◽  
pp. 3883-3899
Keyword(s):  
Inhibitory Activity ◽  
Hibiscus Cannabinus ◽  
Inflammatory Activity ◽  
Scanning Calorimetry ◽  
Disintegration Time ◽  
Test Species ◽  
Powder Formulation ◽  
Anti Inflammatory

Liquisolid approach is a potential method due to the ease in the production process, low cost, and manufacturing process because of their strong flow and compaction properties. The study's main goal is to create a liquisolid powder formulation from the polyherbal extract and evaluate its anti-inflammatory activity. Hibiscus cannabinus, Murraya koenigii, and Tabernaemontana divaricate are used to formulating polyherbal Liquisolid powder formulation to assess their anti-inflammatory action. The formulation is tested for its stability studies and anti-inflammatory activity, both in-vitro and in-vivo. The polyherbal liquisolid powder formulation (F5) has a minimum strength of 1kg/cm 2, while F2 & F6 strength was 4 kg/cm2; FTIR studies showed no interactions between drug and excipients. To confirm the liquisolid powder formulation, further evaluations on friability, hardness, disintegration time, dissolution rate, and Differential scanning calorimetry and X-ray powder diffraction analysis were determined. The polyherbal extract's anti-inflammatory activities were evaluated using the paw edema and cotton pellet method, and the results are found to exhibit its inhibitory activity. The polyherbal extract of Liquisolid powder formulation was evaluated, and it showed a promising inhibitory activity to overcome the inflammation in the test species. Hence it can be used as an alternative therapy for curing inflammation.

scholarly journals Colchicine Induced Mutation in Nigella sativa Plant for the Assessment of Morpho-Physiological and Biochemical Parameter Vis-A-Vis In Vitro Anti-Inflammatory Activity

2021 ◽  
Vol 15 (1) ◽  
pp. 173-182
Author(s):  
Garima Gupta ◽  
Anjuman Gul Memon ◽  
Brijesh Pandey ◽  
Mohd Sajid Khan ◽  
Mohammed Shariq Iqbal ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Nigella Sativa ◽  
Colchicine Treatment ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
P Value ◽  
Induced Mutation ◽  
Anti Inflammatory ◽  
Physiological And Biochemical

Background: Nigella sativa (NS), an herbaceous medicinal plant recognized for its diverse beneficial applications as a spice and traditional medicine. Objective: The present study was targeted to explore the antioxidant potential of Nigella sativa in response to colchicine-induced mutation. The stress condition brought due to mutation may affect the medicinal value (anti-inflammatory activity) of the plant. Method: Nigella sativa seeds were imperiled to colchicine treatment at various concentrations viz. 0.00625, 0.0125, 0.025, 0.05 and 0.1% subjected for analysis. Result: The colchicine treated plant (polyploid/ mutant) at 0.025% concentrations showed significant variation at morpho-physiological and biochemical level with respect to control (p value < 0.05). At the morphological level, the plant showed enlargement of shoot length (33.760±2.295mm), root length (13.546±1.535 mm), and leaf area (22.836±1.524 mm2). The analysis of seeds showed enhanced seeds per pod (49.333±4.163), weight of seeds (2.810±0.010g), length (3.133±0.089mm), and width (1.123±0.044mm) when compared with control. The physiological parameters also showed significant enhancement for stomatal index (35.456±4.751%), chlorophyll A (9.053±0.865 µg/gfw), chlorophyll B (4.990±0.763 µg/gfw), and total carotene content (773.190±5.906 µg/gfw). However, the fresh weight/ dry weight ratio (10.738±3.031) was found to be deprived. Furthermore, biochemical parameters viz. total flavonoid (seeds 1.973±0.134; plant 1.703 ± 0.064 mg eqv QE/g of tissue), total phenolic (seeds 15.690±1.495; plant 8.220±0.070 mg eqv GA/g of tissue), total carotene (seeds nil; plant 773.190±5.906 µg/gfw), and total antioxidant (seeds 0.445±0.102; plant 0.386±0.010 mM eqv AA/g tissue) were significantly elevated at 0.025% of colchicine treatment. When the in vitro anti-inflammatory activity was targeted, a significant escalation was observed for inhibition of albumin denaturation (97.466±2.835%), proteinase inhibitory activity (62.290±6.475%), heat-induced hemolysis (89.873±3.533%), hypotonicity induced hemolysis (92.572±3.527%), anti-lipoxygenase activity (96.010±3.098%), and cyclooxygenase inhibitory activity (68.296±3.920%) at 500µg/mL concentration of extract. Conclusion: Thus, it can be concluded that 0.025% of colchicine can induce significant (p value < 0.05) mutation in the Nigella sativa plant, which may lead to alterations at morpho-physiological and biochemical levels. Such treatment induces stress in the plant and leads to elevated antioxidant levels. This in turn elevates the therapeutic potential of the plant. Hence, our study is a novel and open-ended finding to explore various other medical properties of the plant with respect to colchicine-induced mutation.

scholarly journals 2-(Piperidin-4-yl)acetamides as Potent Inhibitors of Soluble Epoxide Hydrolase with Anti-Inflammatory Activity

2021 ◽  
Vol 14 (12) ◽  
pp. 1323
Author(s):  
Juan Martín-López ◽  
Sandra Codony ◽  
Clara Bartra ◽  
Christophe Morisseau ◽  
María Isabel Loza ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Epoxide Hydrolase ◽  
Inflammatory Diseases ◽  
Soluble Epoxide Hydrolase ◽  
Inflammatory Activity ◽  
Epoxyeicosatrienoic Acids ◽  
Pharmacological Inhibition ◽  
Anti Inflammatory ◽  
Further Development ◽  
Potent Inhibitory Activity

The pharmacological inhibition of soluble epoxide hydrolase (sEH) has been suggested as a potential therapy for the treatment of pain and inflammatory diseases through the stabilization of endogenous epoxyeicosatrienoic acids. Numerous potent sEH inhibitors (sEHI) have been developed, however many contain highly lipophilic substituents limiting their availability. Recently, a new series of benzohomoadamantane-based ureas endowed with potent inhibitory activity for the human and murine sEH was reported. However, their very low microsomal stability prevented further development. Herein, a new series of benzohomoadamantane-based amides were synthetized, fully characterized, and evaluated as sEHI. Most of these amides were endowed with excellent inhibitory potencies. A selected compound displayed anti-inflammatory effects with higher effectiveness than the reference sEHI, TPPU.

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