In vitro susceptibility of Entamoeba histolytica and Giardia lamblia to plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders

2006 ◽  
Vol 108 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Fernando Calzada ◽  
Lilian Yépez-Mulia ◽  
Abigaíl Aguilar
Keyword(s):  
Traditional Medicine ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Gastrointestinal Disorders ◽  
In Vitro Susceptibility

scholarly journals Inhibición del crecimiento de Giardia Lamblia por acción del extracto acuoso y metanólico de semillas de Cucurbita Pepo / Giardia lamblia growth inhibition by action of the aqueous and methanolic extract of cucurbita pepo seed

2015 ◽  
Vol 1 (1) ◽  
pp. 121
Author(s):  
María Porfiria Barrón González ◽  
Ramón Gerardo Rodríguez Garza ◽  
Yadira Quiñones Gutiérrez
Keyword(s):  
Growth Inhibition ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Cucurbita Pepo ◽  
Methanolic Extract ◽  
Taenia Solium

Giardia lamblia es el protozoario parásito causante de la giardiasis, la cual se caracteriza por molestia abdominal, pérdida de peso y desnutrición. La droga de elección para su tratamiento es el metronidazol sin embargo, presenta diversos efectos secundarios adversos en el paciente. Por otra parte, en la herbolaria se conoce a la semilla de Cucurbita pepo por sus propiedades desparasitante, principalmente sobre Entamoeba histolytica y Taenia solium.  Por lo anterior se planteó la hipótesis de que las semillas de C. pepo contienen principios activos capaces de inhibir el crecimiento de G. lamblia bajo condiciones axénicas in vitro. El objetivo de esta investigación fue evaluar la actividad biológica del extracto acuoso y metanólico de las semillas de  C. pepo sobre el crecimiento de G. lamblia.  Tanto el extracto metanólico como el acuoso se identificaron siete grupos funcionales y la actividad antioxidante no fue significativa. En el extracto metanólico se observó mayor efecto giardicida seguido del acuoso, esta diferencia puede deberse a las saponinas que se encuentran sólo en el extracto metanólico. Los resultados sugieren que las semillas contienen principios activos que pueden emplearse para la investigación de nuevos tratamientos para la  giardiasis.

scholarly journals In vitro activity of selected Ghanaian medicinal plants against parasites: Giardia lamblia, Entamoeba histolytica and Naegleria fowleri

2017 ◽  
Vol 11 (23) ◽  
pp. 279-283 ◽  
Author(s):  
Kyere-Davies Gertrude ◽  
Agyare Christian ◽  
Duah Boakye Yaw ◽  
Bains Trpta ◽  
M. Suzuki Brian ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri

scholarly journals Virtual Screening of FDA-Approved Drugs against Triose Phosphate Isomerase from Entamoeba histolytica and Giardia lamblia Identifies Inhibitors of Their Trophozoite Growth Phase

2021 ◽  
Vol 22 (11) ◽  
pp. 5943
Author(s):  
Alfredo Juárez-Saldivar ◽  
Elizabeth Barbosa-Cabrera ◽  
Edgar E. Lara-Ramírez ◽  
Alma D. Paz-González ◽  
Ana V. Martínez-Vázquez ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Triosephosphate Isomerase ◽  
Glycolytic Enzyme ◽  
Public Health Issue ◽  
Intestinal Protozoan ◽  
Approved Drugs ◽  
Fda Approved Drugs

Infectious diseases caused by intestinal protozoan, such as Entamoeba histolytica (E. histolytica) and Giardia lamblia (G. lamblia) are a worldwide public health issue. They affect more than 70 million people every year. They colonize intestines causing primarily diarrhea; nevertheless, these infections can lead to more serious complications. The treatment of choice, metronidazole, is in doubt due to adverse effects and resistance. Therefore, there is a need for new compounds against these parasites. In this work, a structure-based virtual screening of FDA-approved drugs was performed to identify compounds with antiprotozoal activity. The glycolytic enzyme triosephosphate isomerase, present in both E. histolytica and G. lamblia, was used as the drug target. The compounds with the best average docking score on both structures were selected for the in vitro evaluation. Three compounds, chlorhexidine, tolcapone, and imatinib, were capable of inhibit growth on G. lamblia trophozoites (0.05–4.935 μg/mL), while folic acid showed activity against E. histolytica (0.186 μg/mL) and G. lamblia (5.342 μg/mL).

In Vitro susceptibility ofHelicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders

2005 ◽  
Vol 19 (11) ◽  
pp. 988-991 ◽  
Author(s):  
Gail B. Mahady ◽  
Susan L. Pendland ◽  
Adenia Stoia ◽  
Frank A. Hamill ◽  
Daniel Fabricant ◽  
...  
Keyword(s):  
Gastrointestinal Disorders ◽  
In Vitro Susceptibility ◽  
Botanical Extracts

scholarly journals Screening for Antimicrobial Activities of Metanollic Extracts of Aloe vera and Hyptis suaveolens against Co-infections of Giardia lamblia and Salmonella among Diarrhoeagenic Children

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Omammegbe Abdulrahman ◽  
Adamu B. Samaila ◽  
S. M. Panda ◽  
Abdullahi Aliyu ◽  
M. R. Sahal
Keyword(s):  
Antibacterial Activity ◽  
Giardia Lamblia ◽  
Aloe Vera ◽  
Antimicrobial Activities ◽  
Inhibitory Effects ◽  
Zone Of Inhibition ◽  
In Vitro Susceptibility ◽  
Methanol Extracts ◽  
Hyptis Suaveolens

The antimicrobial and Phytochemicals activities of methanol extracts obtained from Aloe vera and Hyptis suaveolens plants were investigated individually and combined in an attempt to evaluate their medicinal potentials and efficacies on protozoan; Giardia lamblia and bacteria; Salmonella species as co-infections causing diarrhoea in under five populations in Bauchi State, Nigeria. The phytochemical screening revealed the presence of saponins, tannins, alkaloids, flavonoids, terpenoids, alkaloids, phenols. Antimicrobial activity was determined against Giardia lamblia and Salmonella species; anti-giardial activity, an in-vitro susceptibility assays method was performed and antibacterial activity was carried out by Kirby-Bauer method. The parasites mortality was determined by counting in hemocytometer under a light microscope and the zone of inhibition diameter produced against the bacteria were determined, expressed as mean ±SEM (Standard Error of Mean) and the differences between means were statistically analyzed and compared. The results obtained showed that methanol extracts of Aloe vera and Hyptis suaveolens singly used and in combinations had inhibitory effects on Giardia lamblia and Salmonella species. In all cases, the extractions, plants, concentrations and time were determinant factors for the anti-giardial and antibacterial activity. Anti-giardial activity was best recorded with extracts of Aloe vera which showed anti-giardial activity of (0.002±0.553), and activity was greater in combined Aloe vera and Hyptis suaveolens which showed anti-giardial activity of (0.002±0.679). Also, antibacterial activity of methanol extracts of these plants on Salmonella species, showed higher zone of inhibitions diameter with extracts of Aloe vera (0.895±20.17) and the efficacy becomes higher with the combined Aloe vera and Hyptis suaveolens which zone of inhibitions is (0.423±27.50).

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