Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine

2006 ◽  
Vol 107 (3) ◽  
pp. 418-423 ◽  
Author(s):  
Gulcan Avcı ◽  
Esra Kupeli ◽  
Abdullah Eryavuz ◽  
Erdem Yesilada ◽  
Ismail Kucukkurt
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Activity Assessment

scholarly journals ANTIOXIDANT ACTIVITY ASSESSMENT OF TAMUS COMMUNIS L. ROOTS

2016 ◽  
Vol 8 (12) ◽  
pp. 64 ◽  
Author(s):  
Zerargui Fatima ◽  
Baghiani Abderrahmane ◽  
Khennouf Seddik ◽  
Arrar Lekhmici
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Antioxidant Potential ◽  
Standard Drug ◽  
Activity Assessment ◽  
C 50 ◽  
Β Carotene

<p><strong>Object: </strong>The aim of this study was to evaluate the antioxidant activities of <em>Tamus communis</em> L. (<em>T .communis</em>) roots. This plant is used in folk medicine in the treatment of rheumatism, arthritis and lumbago.</p><p><strong>Methods</strong>: The extraction of polyphenols has been followed by the determination of total polyphenols and flavonoids. The methanol extract (MeOHE) was chromatographied on a silica gel column. The column was eluted with chloroform and then with chloroform /methanol mixtures of increasing polarity, 6 main fractions (FI--FVI)) were collected and grouped according to their TLC (Thin layer chromatography). Several methods were used to evaluate the <em>in vitro</em> antioxidant activity such as DPPH (2,2'-diphenyl-1-picrylhydrazy), bleaching of β-carotene for 24 hours, lipid peroxidation and anti-hemolytic activity. An <em>in vivo</em> approach was carried out on mice treated with CE (crud extract) at a dose of 100 mg / Kg / day for 21 consecutive days, and one group was treated with vitamin C (Vit C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood.</p><p><strong>Results</strong>: The results showed that the highest level of polyphenols was recorded in FII (73.143±0.009<strong> </strong>mg GA-Eq/g extract (Gallic Acid Equivalent)) / g of extract) followed by MeOHE (69.786±0.10 mg GA-Eq/g extract). Total flavonoid contents of extracts / fractions showed that MeOHE contains the highest level. Inhibition of DPPH radical showed that MeOHE (0.1187 ± 0.025 mg / ml) and CE (0.2363 ± 0.019 mg / ml) have the most efficient antioxidant activity.  All the extracts were capable of inhibiting the bleaching of β-carotene by scavenging linoleate derived free radicals, and all the studied extracts have a very important antihemolytic activity (p&lt;0.001) and revealed an extension of hemolysis half time from 70.34±2.15 min of CTL (AAPH (2,2-azo- bis (2-amidinopropane) dihydrochloride)) and 207 ± 5.65 min for FI. The in vivo assay was</p><p>exhibited a significant decrease (54.09%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (47.30% and 46.87% respectively).</p><p><strong>Conclusion</strong>: These findings suggest that the extract obtained from <em>T.  communis</em> have active substances contributing to the increase in natural antioxidant potential.</p>

Apoptosis Caused by Triterpenes and Phytosterols and Antioxidant Activity of an Enriched Flavonoid Extract from Passiflora mucronata

2019 ◽  
Vol 18 (10) ◽  
pp. 1405-1416 ◽  
Author(s):  
Isabel C.V. da Silva ◽  
Goran N. Kaluđerović ◽  
Pollyana F. de Oliveira ◽  
Denise O. Guimarães ◽  
Carla H. Quaresma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Oleanolic Acid ◽  
Folk Medicine ◽  
Hexane Fraction ◽  
Cytotoxicity Test ◽  
B16f10 Cell ◽  
Dpph Method ◽  
Melanoma B16f10 ◽  
First Time

Background: P. mucronata (Pm) comes from South America, Brazil and is characterized as “Maracujá de Restinga”. It is used in folk medicine for its soothing properties and in treating insomnia. Objective: The present study for the first time analyzed the antioxidant and cytotoxicity of the hydroalcoholic leaves extract and fractions from Pm. Method: The cytotoxicity test will be evaluated by different assays (MTT and CV) against human prostate cancer (PC3) and mouse malignant melanoma (B16F10) cell lines, and the antioxidant test by DPPH method. Results: β-Amyrin, oleanolic acid, β-sitosterol and stigmasterol were isolated of the most active, hexane fraction. These substances were tested against the tumor cell lines: β-sitosterol and stigmasterol showed the most relevant activity to PC3 in CV assay and, oleanolic acid to B16F10 by the MTT assay. In addition, it was possible to indicate that the mode of cell death for stigmasterol, presumably is apoptosis. In terms of antioxidant activity, the hydroalcoholic leaves extract presented higher activity (EC50 133.3 µg/mL) compared to the flower (EC50 152.3 µg/mL) and fruit (EC50 207.9 µg/mL) extracts. By the HPLC-MS, it was possible to identify the presence of flavones in the leaf extract (isoschaftoside, schaftoside, isovitexin, vitexin, isoorientin, orientin). Conclusions: P. mucronata hexane fraction showed promising cytotoxic effect against cancer cell lines, and stigmasterol contributes to this activity, inducing apoptosis of these cells. Furthermore, as other Passiflora species, Pm extract showed antioxidant activity and flavones are its major phenolic compounds.

Investigation of Nutritional Contents, Antioxidant and Immunostimulatory Activities of Aqueous Extract from Laguncularia racemosa Leaves

2020 ◽  
Vol 10 ◽  
Author(s):  
Dayane Kelly Dias do Nascimento Santos ◽  
Cristiane Moutinho Lagos de Melo ◽  
Elivânia Maria da Silva ◽  
Vanessa Silva de Almeida ◽  
Iranildo José da Cruz Filho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Proliferation Index ◽  
Laguncularia Racemosa ◽  
High Concentration ◽  
Popular Medicine ◽  
Biological Tests ◽  
Phytochemical Profile

Background: One of the four most incident species in mangrove is the Laguncularia racemosa, plant widely used in popular medicine against inflammation and fever. Objective: Here, L. racemosa was investigated in relation to their phytochemical profile, antioxidant activity, citotoxicity, antimicrobial and immunostimulatory effect. Method: Aqueous extract was obtained from leaves of plant, its phytochemical profile was investigated through UPLC method, the antioxidant assays performed were TAA, DPPH, ABTS, nitrite and lipid peroxidation assay. Antimicrobial assays were made using standard strains. For all biological tests were used mice splenocytes and from these cell cultures were measured cytotoxicity, proliferation index and cytokines production. Results: Laguncularia racemosa leaves showed the presence of ions, proteins, carbohydrates, vitamins and high concentration of phenolic compounds. Antioxidant activities were promoted by aqueous extract, especially in DPPH and NO assays. Extract in 6 µg/mL did not induce significant cell death, stimulated the cell proliferation and the IL-4 production. Moreover, decreases of proinflammatory cytokines IFN-γ, TNF-α and IL-6 were found. Conclusion: The presence of essential nutrients, significant antioxidant activity and immune stimulation confirm the use of this plant in folk medicine against inflammation.

Effect of pyrolysis temperature on volatile products from hazelnut shells: products characteristics and antioxidant activity assessment of liquid products

2021 ◽  
Vol 19 (4) ◽  
pp. 383-391
Author(s):  
Chenxi Zhao ◽  
Yupeng Xing ◽  
Wei Lv ◽  
Juhui Chen ◽  
Xiaogang Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Composition Analysis ◽  
Maximum Output ◽  
Activity Assessment ◽  
Volatile Products ◽  
Liquid Products ◽  
Fuels And Chemicals ◽  
Gas Products ◽  
Combustible Gases ◽  
Hazelnut Shells

Abstract It is being considered to pyrolyze lignin-rich biomass samples (hazelnut shells, HSs) into bio-fuels and chemicals to solve energy shortages and environmental concerns, volatile products (including liquid products and gas products) were produced and characterized from HSs pyrolysis at 400–1000 °C. With the temperature increases, the maximum output of liquid products was up to 35.79% produced at 700 °C, gas products yields increased from 21.82 to 55.46%. Gas chromatography and mass spectrometry (GC–MS) study indicated that liquid products from HSs riched in phenolic compounds, exceed 42% of liquid products and increased as the temperature rises. The application experiment showed that HSs liquid products had a significant role in antioxidant activity, and revealed that not limited to phenols, all compounds containing phenolic hydroxyl structure act as antioxidant. Composition analysis of gas products showed that more combustible gases were produced at the higher temperature, resulted in the significant increase in gas products higher heating value (HHV) from 6.21 to 24.36 MJ/kg.

scholarly journals Bioactive Compounds, Antioxidant Activity, and Biological Effects of European Cranberry (Vaccinium oxycoccos)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 24 ◽  
Author(s):  
Tunde Jurikova ◽  
Sona Skrovankova ◽  
Jiri Mlcek ◽  
Stefan Balla ◽  
Lukas Snopek
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Folk Medicine ◽  
Meat Products ◽  
Biologically Active ◽  
Anticancer Activities ◽  
Fruit Species ◽  
American Cranberry ◽  
Antibacterial And Antifungal ◽  
Active Substances

Lesser known fruits or underutilized fruit species are recently of great research interest due to the presence of phytochemicals that manifest many biological effects. European cranberry, Vaccinium oxycoccos fruit, as an important representative of this group, is a valuable source of antioxidants and other biologically active substances, similar to American cranberry (V. macrocarpon) which is well known and studied. European cranberry fruit is rich especially in polyphenolic compounds anthocyanins (12.4–207.3 mg/100 g fw), proanthocyanins (1.5–5.3 mg/100 g fw), and flavonols, especially quercetin (0.52–15.4 mg/100 g fw), which mostly contribute to the antioxidant activity of the fruit. Small cranberry is also important due to its various biological effects such as urinary tract protection (proanthocyanidins), antibacterial and antifungal properties (quercetin, proanthocyanidins, anthocyanins), cardioprotective (proanthocyanidins) and anticancer activities (proanthocyanidins), and utilization in food (juice drinks, jams, jellies, sauces, additive to meat products) and pharmacological industries, and in folk medicine.

scholarly journals Chemical Composition and Antioxidant Activity of Essential Oils from Leaves of Two Specimens of Eugenia florida DC.

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5848
Author(s):  
Oberdan Oliveira Ferreira ◽  
Celeste de Jesus Pereira Franco ◽  
Everton Luiz Pompeu Varela ◽  
Sebastião Gomes Silva ◽  
Márcia Moraes Cascaes ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antioxidant Capacity ◽  
Secondary Forest ◽  
Folk Medicine ◽  
Gastrointestinal Problems ◽  
Trolox Equivalent ◽  
Almost All ◽  
Floodplain Area

Eugenia florida DC. belongs to the Myrtaceae family, which is present in almost all of Brazil. This species is popularly known as pitanga-preta or guamirim and is used in folk medicine to treat gastrointestinal problems. In this study, two specimens of Eugenia florida (Efl) were collected in different areas of the same region. Specimen A (EflA) was collected in an area of secondary forest (capoeira), while specimen B (EflB) was collected in a floodplain area. The essential oils (EOs) were extracted from both specimens of Eugenia florida by means of hydrodistillation. Gas chromatography coupled to mass spectrometry (GC/MS) was used to identify the volatile compounds present, and the antioxidant capacity of the EOs was determined by antioxidant capacity (AC-DPPH) and the Trolox equivalent antioxidant (TEAC) assay. For E. florida, limonene (11.98%), spathulenol (10.94%) and α-pinene (5.21%) were identified as the main compounds of the EO extracted from sample A, while sample B comprised selina-3,11-dien-6α-ol (12.03%), eremoligenol (11.0%) and γ-elemene (10.70%). This difference in chemical composition impacted the antioxidant activity of the EOs between the studied samples, especially in sample B of E. florida. This study is the first to report on the antioxidant activity of Eugenia florida DC. essential oils.

Antioxidant activity assessment of Yingjisha swe et al mond oil

2022 ◽  
Author(s):  
Wei Zhao ◽  
Liwen Wang ◽  
Fan Yang ◽  
Ning Zhang ◽  
Jiahuan Fan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Activity Assessment

Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidant activity

2017 ◽  
Vol 41 (15) ◽  
pp. 7681-7688 ◽  
Author(s):  
J. Jiménez-López ◽  
A. Ruiz-Medina ◽  
P. Ortega-Barrales ◽  
E. J. Llorent-Martínez
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Folk Medicine ◽  
Liquid Chromatography Mass Spectrometry ◽  
Rosa Rubiginosa ◽  
Chromatography Mass Spectrometry ◽  
Phytochemical Profiles

The consumption of infusions and herbal teas has always been an important part of folk medicine.

scholarly journals Isolasi dan Uji Aktivitas Antioksidan Ekstrak Metanol Dari Buah Senduduk Bulu (Clidemia hirta (L.) D. Don)

2020 ◽  
Vol 17 (2) ◽  
pp. 92-100
Author(s):  
Haiyul Fadhli ◽  
Ihsan Ikhtiarudin ◽  
Putri Lestari
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
White Crystal ◽  
Pure Compound ◽  
Uv Spectrum ◽  
Chromatography Method ◽  
Nmr Spectrum ◽  
Ft Ir ◽  
Vacuum Liquid Chromatography

A study on isolation and antioxidant activity of secondary metabolit of methanol extract of fruit of Senduduk Bulu (Clidemia hirta (L.) D. Don) has been carried out. Senduduk bulu is used for folk medicine to many diseases. Flavonoid and phenolic has been reported to its activity as antioxidant. The aim of this research was to find antioxidant compound and antioxidant activity of this fruit. Isolation works were conducted using Vacuum Liquid Chromatography and Column Chromatography method. Antioxidant activity was determined using DPPH free radical scavenging methods. The isolation works afforded 10 mg of pure compound CHP1 which was obtained as white crystal, with its melting point of 165–167ºC, soluble in chloroform and slightly soluble in methanol. This compound gave positive reaction with Liebermann-Burchard (LB) reagent and concluded as terpenoid. Based on it UV spectrum, this compound showed a λ max of 316 nm. Its FT-IR spectrum showed appearence of some functional groups of OH, C-H aliphatic, C=O ester,C=C alkene, CH bending and C-O. The 1H-NMR spectrum showed the alkene protons and methyl protons which is a characteristic of terpenoid compounds. The pure compound (CHP1) provided a weak antioxidant activity with the IC50  of 327,01µg/mL.

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