Highly sensitive TaqMan RT-PCR assay for detection and quantification of both lineages of West Nile virus RNA

2006 ◽  
Vol 36 (3) ◽  
pp. 177-182 ◽  
Author(s):  
Yanlin Tang ◽  
C. Anne Hapip ◽  
Bin Liu ◽  
Chyang T. Fang
Keyword(s):  
West Nile Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
West Nile ◽  
Virus Rna ◽  
Highly Sensitive ◽  
Detection And Quantification

Establishment and evaluation of real-time PCR for West Nile virus detection

2009 ◽  
Vol 6 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Shi Li-Jun ◽  
Lu Mao-Min ◽  
Li Gang ◽  
Li Cheng-Yao ◽  
Zhang Jin-Gang
Keyword(s):  
West Nile Virus ◽  
Real Time ◽  
Virus Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
West Nile ◽  
Capsid Protein Gene ◽  
Specific Test ◽  
Intercalating Dye ◽  
Polymerase Chain

AbstractA rapid real-time polymerase chain reaction (RT-PCR) for detecting West Nile virus (WNV) was established. Primers were designed according to the sequence of the capsid protein gene of WNV by Primer Premier 5.0. In this way, an inexpensive assay using the intercalating dye SYBR Green I was developed and validated. The amplifying curve showed that this method could successfully amplify 102 copies/μl of the WNV gene, while reference to Japanese encephalitis virus (JEV) and blank control were all negative. Tenfold successive dilutions of positive WNV DNA were used to measure the sensitivity of RT-PCR. The assay system showed high reproducibility with coefficient of variation (CV) <2%. Thus the newly established RT-PCR assay was shown to be a rapid, sensitive and specific test for detecting WNV.

scholarly journals Improved detection of Zika virus RNA in human and animal specimens by a novel, highly sensitive and specific real-time RT-PCR assay targeting the 5′-untranslated region of Zika virus

2017 ◽  
Vol 22 (5) ◽  
pp. 594-603 ◽  
Author(s):  
Jasper Fuk-Woo Chan ◽  
Cyril Chik-Yan Yip ◽  
Kah-Meng Tee ◽  
Zheng Zhu ◽  
Jessica Oi-Ling Tsang ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Untranslated Region ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Virus Rna ◽  
Highly Sensitive

scholarly journals Screening of Mosquitoes for West Nile Virus and Usutu Virus in Croatia, 2015–2020

2021 ◽  
Vol 6 (2) ◽  
pp. 45
Author(s):  
Ana Klobucar ◽  
Vladimir Savic ◽  
Marcela Curman Posavec ◽  
Suncica Petrinic ◽  
Urska Kuhar ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Culex Pipiens ◽  
Rt Pcr ◽  
West Nile ◽  
Usutu Virus ◽  
Culex Pipiens Complex ◽  
Mammalian Origin ◽  
Mosquito Pool ◽  
The City ◽  
Generation Sequencing

In the period from 2015 to 2020, an entomological survey for the presence of West Nile virus (WNV) and Usutu virus (USUV) in mosquitoes was performed in northwestern Croatia. A total of 20,363 mosquitoes were sampled in the City of Zagreb and Međimurje county, grouped in 899 pools and tested by real-time RT-PCR for WNV and USUV RNA. All pools were negative for WNV while one pool each from 2016 (Aedes albopictus), 2017 (Culex pipiens complex), 2018 (Cx. pipiens complex), and 2019 (Cx. pipiens complex), respectively, was positive for USUV. The 2018 and 2019 positive pools shared 99.31% nucleotide homology within the USUV NS5 gene and both clustered within USUV Europe 2 lineage. The next-generation sequencing of one mosquito pool (Cx. pipiens complex) collected in 2018 in Zagreb confirmed the presence of USUV and revealed several dsDNA and ssRNA viruses of insect, bacterial and mammalian origin.

Development of a strand-specific real-time qRT-PCR for the accurate detection and quantitation of West Nile virus RNA

2013 ◽  
Vol 194 (1-2) ◽  
pp. 146-153 ◽  
Author(s):  
Stephanie M. Lim ◽  
Penelope Koraka ◽  
Albert D.M.E. Osterhaus ◽  
Byron E.E. Martina
Keyword(s):  
West Nile Virus ◽  
Real Time ◽  
West Nile ◽  
Qrt Pcr ◽  
Accurate Detection ◽  
Virus Rna

The survey of wild birds for West Nile virus in Poland

2011 ◽  
Vol 14 (4) ◽  
pp. 573-577 ◽  
Author(s):  
J. Niczyporuk ◽  
E. Samorek-Salamonowicz ◽  
W. Kozdruń ◽  
Z. Mizak
Keyword(s):  
West Nile Virus ◽  
Genetic Material ◽  
Early Spring ◽  
Wild Birds ◽  
Rt Pcr ◽  
West Nile ◽  
Late Autumn ◽  
The Brain

The survey of wild birds for West Nile virus in PolandTwo thousand one hundred and forty birds belonging to 39 different species from different locations in Poland were examined. The study has taken place from the early spring till late autumn 2007-2010 when the activity of the mosquitoes was the highest. The brain samples were taken from the birds and whole cellular RNA was isolated, then the RT-PCR and NRT-PCR were performed to detect the presence of West Nile virus (WNV). The obtained results were confirmed by the commercial WNV Kit. No genetic material of WNV was found in the examined samples.

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