Selective monitoring

2021 ◽  
Vol 117 ◽  
pp. 99-129
Author(s):  
Radu Grigore ◽  
Stefan Kiefer
Keyword(s):  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...


The Analyst ◽  
2013 ◽  
Vol 138 (12) ◽  
pp. 3356 ◽  
Author(s):  
Anup Kumar ◽  
Alok K. Singh ◽  
Tarkeshwar Gupta

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3143 ◽  
Author(s):  
Li Jia ◽  
Lingling Fu ◽  
Xiaoyan Wang ◽  
Wenzhi Yang ◽  
Hongda Wang ◽  
...  

The analytical platform UHPLC/Q-Orbitrap-MS offers a solution to quality investigation of TCM with high definiteness. Using Erzhi Pill (EZP) as a case, we developed UHPLC/Q-Orbitrap-MS based approaches to achieve systematic multicomponent identification and rapid authentication. Comprehensive multicomponent characterization of EZP was performed by negative/positive switching data-dependent high-energy collision-induced dissociation-MS2 (HCD-MS2) after 25 min chromatographic separation. By reference compounds comparison, elemental composition analysis, fragmentation pathways interpretation, and retrieval of an in-house library, 366 compounds were separated and detected from EZP, and 96 thereof were structurally characterized. The fingerprints of two component drugs (Ligustri Lucidi Fructus, LLF; Ecliptae Herba, EH) for EZP were analyzed under the same LC-MS condition by full scan in negative mode. In combination with currently available pharmacological reports, eight compounds were deduced as the ‘identity markers’ of EZP. Selective ion monitoring (SIM) of eight marker compounds was conducted to authenticate six batches of EZP samples. Both LLF and EH could be detected from all EZP samples by analyzing the SIM spectra, which could indicate their authenticity. Conclusively, UHPLC/Q-Orbitrap-MS by rapid polarity switching could greatly expand the potency of untargeted profiling with high efficiency, and SIM of multiple chemical markers rendered a practical approach enabling the authentication of TCM formulae.


the dosages used to kill mosquitoes, non-target organisms are safe. Both of these products, especially Bacillus, break down quickly and should therefore present no hazard to water quality. Given that a site such as Big Bay may become a mecca for those wishing to swim, sail or even fish, a surveillance programme and some environmental modifications are necessary. The deeper open waters of this bay coupled with a vegetation-free foreshore as a buffer zone, perhaps 400 m on either side of a swimming zone, should minimize or even negate swimmer’s itch. Adjacent Hydrilla and other macrophytes will require clearing as these will also present a physical hazard to swimmers and watercraft. The monitoring programmes could ideally be done three to four weeks prior to extensive public usage to allow time for any remedial action. The prevalence of key mosquito species and of large Austropeplea (and Gyraulus and Amerianna) snails can be established quickly as can cercarial infection in the snails. If it is found necessary to establish infection rates in mosquitoes, the newly developed Ross River and Barmah Forest virus testing procedures using mosquito cell cultures and enzyme immunosorbent assay (Oliveira et al. 1995) would require six days processing time. This offers considerable economy over previous methods using intracerebral inoculation of baby suckling mice. We would suggest that prior to selected recreational events, especially those from March to May, the Water Supply Board should initiate the action shown in Figure 9.6. The information supplied in response to a request should be communicated to recreational users to ensure that they are aware of the risks. Perhaps mosquito, arbovirus, and schistosome status could be displayed in the same way as fire hazard status is commonly indicated. It would be remiss of us to generate the impression that we had all the answers to the Ross River dam. The stage 2A lake and its surrounds are undergoing a process of ecological change and realization of this must remain paramount. There are issues relating to mosquito biology and behaviour and to do with snail ecology generally that would repay further study. Thus further selective monitoring and research should not be forsaken. References

1998 ◽  
pp. 155-157

2009 ◽  
Vol 404 (2) ◽  
pp. 144-148 ◽  
Author(s):  
Bo Li ◽  
Inger Byrjalsen ◽  
Paul Glendenning ◽  
Dennis B. Henriksen ◽  
Hans C. Hoeck ◽  
...  

2012 ◽  
Vol 25 (0) ◽  
pp. 40
Author(s):  
Alexis Pérez-Bellido ◽  
Joan López-Moliner ◽  
Salvador Soto-Faraco

Prior knowledge about the spatial frequency (SF) of upcoming visual targets (Gabor patches) speeds up average reaction times and decreases standard deviation. This has often been regarded as evidence for a multichannel processing of SF in vision. Multisensory research, on the other hand, has often reported the existence of sensory interactions between auditory and visual signals. These interactions result in enhancements in visual processing, leading to lower sensory thresholds and/or more precise visual estimates. However, little is known about how multisensory interactions may affect the uncertainty regarding visual SF. We conducted a reaction time study in which we manipulated the uncertanty about SF (SF was blocked or interleaved across trials) of visual targets, and compared visual only versus audio–visual presentations. Surprisingly, the analysis of the reaction times and their standard deviation revealed an impairment of the selective monitoring of the SF channel by the presence of a concurrent sound. Moreover, this impairment was especially pronounced when the relevant channels were high SFs at high visual contrasts. We propose that an accessory sound automatically favours visual processing of low SFs through the magnocellular channels, thereby detracting from the potential benefits from tuning into high SF psychophysical-channels.


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