Quantitative analyses of individual γ-Oryzanol (Steryl Ferulates) in conventional and organic brown rice (Oryza sativa L.)

2012 ◽  
Vol 55 (3) ◽  
pp. 337-343 ◽  
Author(s):  
Jeong-Yong Cho ◽  
Hyoung Jae Lee ◽  
Gee An Kim ◽  
Gwi Deok Kim ◽  
You Seok Lee ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Brown Rice ◽  
Steryl Ferulates ◽  
Quantitative Analyses

scholarly journals Subcellular Localization and Vesicular Structures of Anthocyanin Pigmentation by Fluorescence Imaging of Black Rice (Oryza sativa L.) Stigma Protoplast

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Subcellular Localization ◽  
Oryza Sativa L ◽  
Scientific Evidence ◽  
Brown Rice ◽  
The Body ◽  
Central Vacuole ◽  
In Planta ◽  
Black Rice

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.

Polymer-coated manganese fertilizer and its combination with lime reduces cadmium accumulation in brown rice (Oryza sativa L.)

2021 ◽  
Vol 415 ◽  
pp. 125597
Author(s):  
Gaoxiang Huang ◽  
Changfeng Ding ◽  
Naijia Guo ◽  
Mingjun Ding ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Brown Rice ◽  
Cadmium Accumulation

scholarly journals Brown rice (Oryza sativa L. cv. Hiami) extract promotes cellular growth by upregulation of GH and IGF-1 expression and secretion

2016 ◽  
Vol 25 (1) ◽  
pp. 335-339
Author(s):  
Hyun-seok Oh ◽  
Se Kwan Oh ◽  
Jum Seek Lee ◽  
June Seek Choi ◽  
Sung-Joon Lee
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Brown Rice ◽  
Cellular Growth

A preliminary study of the protease activities in germinating brown rice (Oryza sativa L.)

2010 ◽  
Vol 91 (5) ◽  
pp. 915-920 ◽  
Author(s):  
Cuijuan Li ◽  
Xiaohong Cao ◽  
Zhenxin Gu ◽  
Huanbin Wen
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Brown Rice ◽  
Preliminary Study

scholarly journals The Effect of Degree of Milling on the Nutraceutical Content in Ecofriendly and Conventional Rice (Oryza sativa L.)

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1297
Author(s):  
Seung-Hyun Kim ◽  
Yu-Jin Yang ◽  
Ill-Min Chung
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Activities ◽  
Oryza Sativa L ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Brown Rice ◽  
Stepwise Discriminant Analysis ◽  
Rice Grains ◽  
Degree Of Milling

We investigated the effects of the type of rice and degree of milling (DOM) on the nutraceutical content and antioxidant activity of rice (Oryza sativa L.). The fatty acid (FA), vitamin E homolog, and phenolic contents in organic (OR), pesticide-free (PFR), and conventional rice (CR) decreased significantly with an increase in the DOM of rice grains, particularly for a DOM of 7 and 9 (p < 0.05). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity also decreased with the DOM; particularly, this activity decreased significantly, by approximately 60%, in rice grains with a DOM between 7 and 11, as compared to that of brown rice (p < 0.05). α-Tocopherol (r = 0.854) and p-coumaric acid (r = 0.501) showed the strongest correlation with DPPH activity in each chemical group. Stepwise discriminant analysis enabled the correct original and cross-validated classification of 87.0% and 81.5% of rice types, respectively. Additionally, the original and cross-validated classification of rice DOM levels showed that, overall, 93.8% and 92.6% of rice samples were correctly classified. Our findings reveal variations in the nutraceutical levels and antioxidant activities in rice grains based on the rice type and DOM, which can help improve the nutritional evaluation of human-health-promoting rice grains.

Effects of parboiling, storage and cooking on the levels of tocopherols, tocotrienols and γ-oryzanol in brown rice (Oryza sativa L.)

2013 ◽  
Vol 50 (2) ◽  
pp. 676-681 ◽  
Author(s):  
Cristina de Simone Carlos Iglesias Pascual ◽  
Isabel Louro Massaretto ◽  
Fabiana Kawassaki ◽  
Rosa Maria Cerdeira Barros ◽  
José Alberto Noldin ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Brown Rice
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