Evaluation of thermomechanical pretreatment for enzymatic hydrolysis of pure microcrystalline cellulose and cellulose from Brewers’ spent grain

2011 ◽  
Vol 54 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Guillaume Pierre ◽  
Frédéric Sannier ◽  
Romain Goude ◽  
Armelle Nouviaire ◽  
Zoulikha Maache-Rezzoug ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microcrystalline Cellulose ◽  
Spent Grain ◽  
Hydrolysis Of

Effect of phosphoric acid pretreatment on enzymatic hydrolysis of microcrystalline cellulose

2010 ◽  
Vol 28 (5) ◽  
pp. 613-619 ◽  
Author(s):  
Juanhua Zhang ◽  
Beixiao Zhang ◽  
Jingqiang Zhang ◽  
Lu Lin ◽  
Shijie Liu ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Phosphoric Acid ◽  
Microcrystalline Cellulose ◽  
Acid Pretreatment ◽  
Hydrolysis Of

scholarly journals Enzymatic hydrolysis of beer brewers' spent grain and the influence of pretreatments

1987 ◽  
Vol 30 (5) ◽  
pp. 668-671 ◽  
Author(s):  
G. Beldman ◽  
J. Hennekam ◽  
A. G. J. Voragen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Spent Grain ◽  
Hydrolysis Of

scholarly journals Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 4898-4911
Keyword(s):  
Enzymatic Hydrolysis ◽  
Physicochemical Properties ◽  
Corn Stover ◽  
Microcrystalline Cellulose ◽  
Residual Lignin ◽  
Surface Charges ◽  
Pretreated Corn Stover ◽  
Lignin Fraction ◽  
Isolation Methods ◽  
Hydrolysis Of

Lignin has been shown to be a recalcitrance factor in many biomass conversion studies. To better understand the effects of lignin on cellulose conversion, different lignin fractions were extracted from the same dilute acid pretreated corn stover by three sequential isolation methods, namely ethanol extraction, dioxane extraction, and enzyme purification. The physicochemical properties of each lignin fraction including molecular weight distribution, surface area, surface charge, and other structural features varied, depending on the isolation methods. All three lignin fractions had negative surface charges, and ethanol-extracted lignin carried the highest surface charges, followed by dioxane-extracted lignin and cellulase-purified residual lignin. These physicochemical properties of lignin fractions also resulted in different extent of inhibitory effects on enzymatic hydrolysis of microcrystalline cellulose (MCC). Dioxane-extracted lignin exhibited the highest inhibitory effect on glucose release from MCC, followed by the cellulase-purified residual lignin fraction and ethanol-extracted lignin. Furthermore, lignin fractions with higher contents of syringyl (S) substructure and β-O-4 aryl ether interunit linkages showed a stronger negative effect on cellulase hydrolysis of MCC.

scholarly journals Study of Pretreatment and Enzymatic Hydrolysis on Microcrystalline Cellulose and HVS A4 Paper in Pressurized CO2 Media

2015 ◽  
Vol 9 (7) ◽  
pp. 16 ◽  
Author(s):  
Ian Eka Pramudita ◽  
Marcella Lauditta Noviana ◽  
Henky Muljana
Keyword(s):  
High Performance Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Microcrystalline Cellulose ◽  
Levulinic Acid ◽  
High Performance ◽  
Model Compound ◽  
Bioethanol Production ◽  
Fixed Temperature ◽  
Pre Treatment ◽  
Hydrolysis Of

The aim of this work is to investigate the potential used of pressurized CO2 in the pre-treatment and the enzymatic hydrolysis of waste HVS A4 paperto produce a monomeric sugars (C5 and C6 sugars) which is a precursor for bioethanol production. Prior to the utilization of waste HVS A4 paper, the microcrystalline cellulose and HVS A4 paper were first used in the experiments as model compound in order to gain better insights of the process. The experiment consists of two main parts which are the preliminary experimentsto determine the best pretreatment conditionsbetween two selected pressure values (80 and 100 bar) at a fixed temperature (T = 75 oC) and the enzymatic hydrolysis experiments. In the latter, the microcrystalline and HVS A4 paper were hydrolyzed at different pressures (100 bar, 125 bar, and 150 bar) and at different cellulase intakes (1% (v/v), 3% (v/v), and 5% (v/v)) with a fixed temperature (50°C). The hydrolyzed products were analyzed with a High Performance Liquid Chromatography (HPLC) to quantify the monomeric sugars and to determine the presence of the side products (furfural, HMF and levulinic acid). Within the experimental range, a maximum glucose concentration of 7602.35 ppm and 4560.79 ppm are obtained for microcrystalline and HVS A4 paper, respectively. In addition, there are no furfural, HMF and levulinic acid detected in the products This study shows a potential used of pressurized CO2 in the pretreatment and enzymatic hydrolysis of the model compound and gives a better insight for further application.

Enzymatic hydrolysis of different allomorphic forms of microcrystalline cellulose

Cellulose ◽  
2011 ◽  
Vol 18 (6) ◽  
pp. 1527-1541 ◽  
Author(s):  
Diana Ciolacu ◽  
Selestina Gorgieva ◽  
Daniel Tampu ◽  
Vanja Kokol
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microcrystalline Cellulose ◽  
Hydrolysis Of

scholarly journals Optimization ofArundo donaxSaccharification by (Hemi)cellulolytic Enzymes fromPleurotus ostreatus

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Rossana Liguori ◽  
Elena Ionata ◽  
Loredana Marcolongo ◽  
Luciana Porto de Souza Vandenberghe ◽  
Francesco La Cara ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Experimental Design ◽  
Enzymatic Hydrolysis ◽  
Microcrystalline Cellulose ◽  
Significant Influence ◽  
Cellulolytic Enzymes ◽  
Factorial Experimental Design ◽  
Screening Design ◽  
Commercial Enzymes ◽  
Hydrolysis Of

An enzymatic mixture of cellulases and xylanases was produced byPleurotus ostreatususing microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis ofArundo donaxbioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreatedA. donax. As the most significant influence during the enzymatic hydrolysis ofA. donaxwas exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion byP. ostreatuscellulase and xylanase was analyzed by a 33factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.

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