scholarly journals Recombinant platelet-derived growth factor-BB and hyaluronic acid stimulates knee cartilage regeneration by forming higher chondrocytes count and lower YKL-40 level in rats model

2020 ◽  
Vol 11 ◽  
pp. S76-S79
Author(s):  
I Ketut Suyasa ◽  
Anak Agung Wiradewi Lestari ◽  
I Gusti Ngurah Yudhi Setiawan
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factor ◽  
Cartilage Regeneration ◽  
Platelet Derived Growth Factor ◽  
Knee Cartilage

scholarly journals Recombinant platelet derived growth factor-BB and hyaluronic acid effect in rat osteoarthritis models

2019 ◽  
Vol 16 (3) ◽  
pp. 230-233
Author(s):  
I Gusti Ngurah Yudhi Setiawan ◽  
I Ketut Suyasa ◽  
Putu Astawa ◽  
I Wayan Suryanto Dusak ◽  
I Ketut Siki Kawiyana ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Acid Effect

scholarly journals Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues

2022 ◽  
Vol 23 (2) ◽  
pp. 924
Author(s):  
Julia Hauptstein ◽  
Leonard Forster ◽  
Ali Nadernezhad ◽  
Jürgen Groll ◽  
Jörg Teßmar ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Cartilage Regeneration ◽  
Cartilaginous Tissues ◽  
Dual Stage ◽  
Tgf Β1

In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance. Growth factors covalently bound in non-printable hydrogels have been shown to effectively promote chondrogenesis. However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking. Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF‑β1). Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF‑β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF‑β1. This was substantiated with regard to early TGF‑β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness. Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF‑β1 maintained its functionality after 3D printing. Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration. Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.

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