scholarly journals Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways

2011 ◽  
Vol 155 (2) ◽  
pp. 134-143 ◽  
Author(s):  
Janette Contreras ◽  
Ahmed Y.O. Elnagar ◽  
Sarah F. Hamm-Alvarez ◽  
Julio A. Camarero
Keyword(s):  
Cellular Uptake ◽  
Endocytic Pathways

siRNA versus pharmacological inhibition of endocytic pathways for studying cellular uptake of cell penetrating peptides and other drug delivery vectors

2010 ◽  
Vol 15 (23-24) ◽  
pp. 1103-1103
Author(s):  
Monerah H. Al-Soraj ◽  
Catherine L. Watkins ◽  
Dries Vercauteren ◽  
Stefaan De Smedt ◽  
Kevin Braeckmans ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cellular Uptake ◽  
Cell Penetrating Peptides ◽  
Pharmacological Inhibition ◽  
Cell Penetrating ◽  
Endocytic Pathways

scholarly journals siRNA Inhibition of Endocytic Pathways to Characterize the Cellular Uptake Mechanisms of Folate-Functionalized Glycol Chitosan Nanogels

2015 ◽  
Vol 12 (6) ◽  
pp. 1970-1979 ◽  
Author(s):  
Paula Pereira ◽  
Sílvia S. Pedrosa ◽  
Jennifer M. Wymant ◽  
Edward Sayers ◽  
Alexandra Correia ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Glycol Chitosan ◽  
Uptake Mechanisms ◽  
Endocytic Pathways

siRNA versus pharmacological inhibition of endocytic pathways for studying cellular uptake of cell penetrating peptides

2010 ◽  
Vol 148 (1) ◽  
pp. e86-e87 ◽  
Author(s):  
Monerah H. Al-Soraj ◽  
Catherine L. Watkins ◽  
Dries Vercauteren ◽  
Stefaan C. De Smedt ◽  
Kevin Braeckmans ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Cell Penetrating Peptides ◽  
Pharmacological Inhibition ◽  
Cell Penetrating ◽  
Endocytic Pathways

scholarly journals The role of endocytic pathways in cellular uptake of plasma non-transferrin iron

Haematologica ◽  
2011 ◽  
Vol 97 (5) ◽  
pp. 670-678 ◽  
Author(s):  
Y.-S. Sohn ◽  
H. Ghoti ◽  
W. Breuer ◽  
E. Rachmilewitz ◽  
S. Attar ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Endocytic Pathways

scholarly journals Ultrastructural Analysis of Vesicular Transport in Electrotransfection

2018 ◽  
Vol 24 (5) ◽  
pp. 553-563 ◽  
Author(s):  
Liangli Wang ◽  
Sara E. Miller ◽  
Fan Yuan
Keyword(s):  
Endothelial Cells ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Intracellular Transport ◽  
Umbilical Vein ◽  
Morphological Evidence ◽  
Common Pathway ◽  
Ultrastructural Evidence ◽  
Umbilical Vein Endothelial Cells ◽  
Endocytic Pathways

AbstractEmerging evidence from various studies indicates that plasmid DNA (pDNA) is internalized by cells through an endocytosis-like process when it is used for electrotransfection. To provide morphological evidence of the process, we investigated ultrastructures in cells that were associated with the electrotransfected pDNA, using immunoelectron microscopy. The results demonstrate that four endocytic pathways are involved in the uptake of the pDNA, including caveolae- and clathrin-mediated endocytosis, macropinocytosis, and the clathrin-independent carrier/glycosylphosphatidylinositol-anchored protein-enriched early endosomal compartment (CLIC/GEEC) pathway. Among them, macropinocytosis is the most common pathway utilized by cells having various pDNA uptake capacities, and the CLIC/GEEC pathway is observed primarily in human umbilical vein endothelial cells. Quantitatively, the endocytic pathways are more active in easy-to-transfect cells than in hard-to-transfect ones. Taken together, our data provide ultrastructural evidence showing that endocytosis plays an important role in cellular uptake and intracellular transport of electrotransfected pDNA.

scholarly journals Determination of Cellular Uptake and Endocytic Pathways

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (4) ◽  
Author(s):  
Jing Gong ◽  
Hong-Xia Wang ◽  
Kam Leong
Keyword(s):  
Cellular Uptake ◽  
Endocytic Pathways

scholarly journals Cell-penetrating peptides and antimicrobial peptides: how different are they?

2006 ◽  
Vol 399 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Sónia Troeira Henriques ◽  
Manuel Nuno Melo ◽  
Miguel A. R. B. Castanho
Keyword(s):  
Cell Membrane ◽  
Antimicrobial Peptides ◽  
Cellular Uptake ◽  
Mammalian Cells ◽  
Cell Penetrating Peptides ◽  
Host Cells ◽  
Cell Penetrating ◽  
New Perspective ◽  
Pathogenic Agents ◽  
Endocytic Pathways

Some cationic peptides, referred to as CPPs (cell-penetrating peptides), have the ability to translocate across biological membranes in a non-disruptive way and to overcome the impermeable nature of the cell membrane. They have been successfully used for drug delivery into mammalian cells; however, there is no consensus about the mechanism of cellular uptake. Both endocytic and non-endocytic pathways are supported by experimental evidence. The observation that some AMPs (antimicrobial peptides) can enter host cells without damaging their cytoplasmic membrane, as well as kill pathogenic agents, has also attracted attention. The capacity to translocate across the cell membrane has been reported for some of these AMPs. Like CPPs, AMPs are short and cationic sequences with a high affinity for membranes. Similarities between CPPs and AMPs prompted us to question if these two classes of peptides really belong to unrelated families. In this Review, a critical comparison of the mechanisms that underlie cellular uptake is undertaken. A reflection and a new perspective about CPPs and AMPs are presented.

Water-soluble amphiphilic ruthenium(ii) polypyridyl complexes as potential light-activated therapeutic agents

2020 ◽  
Vol 56 (65) ◽  
pp. 9332-9335
Author(s):  
Sandra Estalayo-Adrián ◽  
Salvador Blasco ◽  
Sandra A. Bright ◽  
Gavin J. McManus ◽  
Guillermo Orellana ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Polypyridyl Complexes ◽  
Cervical Cancer Cells

Two new water-soluble amphiphilic Ru(ii) polypyridyl complexes were synthesised and their photophysical and photobiological properties evaluated; both complexes showed a rapid cellular uptake and phototoxicity against HeLa cervical cancer cells.

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