scholarly journals In situ mobility of uranium in the presence of nitrate following sulfate-reducing conditions

2016 ◽  
Vol 187 ◽  
pp. 55-64 ◽  
Author(s):  
Charles J. Paradis ◽  
Sindhu Jagadamma ◽  
David B. Watson ◽  
Larry D. McKay ◽  
Terry C. Hazen ◽  
...  
Keyword(s):  
Sulfate Reducing ◽  
Reducing Conditions

scholarly journals Evaluating zinc isotope fractionation under sulfate reducing conditions using a flow-through cell and in situ XAS analysis

2017 ◽  
Vol 203 ◽  
pp. 1-14 ◽  
Author(s):  
Julia H. Jamieson-Hanes ◽  
Heather K. Shrimpton ◽  
Harish Veeramani ◽  
Carol J. Ptacek ◽  
Antonio Lanzirotti ◽  
...  
Keyword(s):  
Isotope Fractionation ◽  
Sulfate Reducing ◽  
Zinc Isotope ◽  
In Situ Xas ◽  
Reducing Conditions ◽  
Flow Through

scholarly journals In Situ BTEX Biotransformation under Enhanced Nitrate- and Sulfate-Reducing Conditions

1997 ◽  
Vol 31 (5) ◽  
pp. 1580-1580 ◽  
Author(s):  
M. Reinhard ◽  
S. Shang ◽  
P. K. Kitanidis ◽  
E. Orwin ◽  
G. D. Hopkins ◽  
...  
Keyword(s):  
Sulfate Reducing ◽  
Reducing Conditions

In Situ BTEX Biotransformation under Enhanced Nitrate- and Sulfate-Reducing Conditions

1997 ◽  
Vol 31 (1) ◽  
pp. 28-36 ◽  
Author(s):  
M. Reinhard ◽  
S. Shang ◽  
P. K. Kitanidis ◽  
E. Orwin ◽  
G. D. Hopkins ◽  
...  
Keyword(s):  
Sulfate Reducing ◽  
Reducing Conditions

Benzene oxidation under sulfate-reducing conditions in columns simulating in situ conditions

2006 ◽  
Vol 18 (5) ◽  
pp. 625-636 ◽  
Author(s):  
Carsten Vogt ◽  
Stefan Gödeke ◽  
Hanns-Christian Treutler ◽  
Holger Weiß ◽  
Mario Schirmer ◽  
...  
Keyword(s):  
Benzene Oxidation ◽  
Sulfate Reducing ◽  
Reducing Conditions ◽  
In Situ Conditions

Cadmium sorption by EPSs produced by anaerobic sludge under sulfate-reducing conditions

2006 ◽  
Vol 138 (3) ◽  
pp. 589-593 ◽  
Author(s):  
Daoyong Zhang ◽  
Jianlong Wang ◽  
Xiangliang Pan
Keyword(s):  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Conditions

scholarly journals Integrative analysis of <i>Geobacter</i> spp. and sulfate-reducing bacteria during uranium bioremediation

2012 ◽  
Vol 9 (3) ◽  
pp. 1033-1040 ◽  
Author(s):  
M. Barlett ◽  
K. Zhuang ◽  
R. Mahadevan ◽  
D. Lovley
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Field Trials ◽  
Second Phase ◽  
Sulfate Reducing ◽  
Organic Electron ◽  
Poor Understanding ◽  
Key Factor ◽  
Reducing Bacteria ◽  
The Impact

Abstract. Enhancing microbial U(VI) reduction with the addition of organic electron donors is a promising strategy for immobilizing uranium in contaminated groundwaters, but has yet to be optimized because of a poor understanding of the factors controlling the growth of various microbial communities during bioremediation. In previous field trials in which acetate was added to the subsurface, there were two distinct phases: an initial phase in which acetate-oxidizing, U(VI)-reducing Geobacter predominated and U(VI) was effectively reduced and a second phase in which acetate-oxidizing sulfate reducing bacteria (SRB) predominated and U(VI) reduction was poor. The interaction of Geobacter and SRB was investigated both in sediment incubations that mimicked in situ bioremediation and with in silico metabolic modeling. In sediment incubations, Geobacter grew quickly but then declined in numbers as the microbially reducible Fe(III) was depleted whereas the SRB grow more slowly and reached dominance after 30–40 days. Modeling predicted a similar outcome. Additional modeling in which the relative initial percentages of the Geobacter and SRB were varied indicated that there was little to no competitive interaction between Geobacter and SRB when acetate was abundant. Further simulations suggested that the addition of Fe(III) would revive the Geobacter, but have little to no effect on the SRB. This result was confirmed experimentally. The results demonstrate that it is possible to predict the impact of amendments on important components of the subsurface microbial community during groundwater bioremediation. The finding that Fe(III) availability, rather than competition with SRB, is the key factor limiting the activity of Geobacter during in situ uranium bioremediation will aid in the design of improved uranium bioremediation strategies.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

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