Simultaneous determination of three flavonoid C-glycosides in mice biosamples by HPLC–ESI-MS method after oral administration of Abrus mollis extract and its application to biodistribution studies

2012 ◽  
Vol 903 ◽  
pp. 68-74 ◽  
Author(s):  
Hao Wang ◽  
Zhenzhou Jiang ◽  
Huibing Du ◽  
Chunyi Liang ◽  
Yuanchao Wang ◽  
...  
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Esi Ms ◽  
Biodistribution Studies ◽  
Abrus Mollis

scholarly journals Simultaneous Determination of Aesculin, Aesculetin, Fraxetin, Fraxin and Polydatin in Beagle Dog Plasma by UPLC-ESI-MS/MS and Its Application in a Pharmacokinetic Study after Oral Administration Extracts of Ledum palustre L.

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2285 ◽  
Author(s):  
Zhibin Wang ◽  
Wenbo Zhu ◽  
Hua Liu ◽  
Gaosong Wu ◽  
Mengmeng Song ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Oral Administration ◽  
Simultaneous Determination ◽  
Maximum Plasma Concentration ◽  
Maximum Plasma ◽  
Beagle Dog ◽  
Esi Ms ◽  
Ledum Palustre ◽  
Dog Plasma

A rapid, simple and sensitive ultra-performance liquid chromatography-electrospray-ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for the simultaneous determination of aesculin, aesculetin, fraxetin, fraxin and polydatin in beagle dog plasma for the first time. Plasma samples were pretreated by protein precipitation with methanol. Chromatographic separation was performed on an Acquity UPLC HSS T3 C18 column (2.1 mm × 100 mm, 1.8 μm) with gradient elution at a flow rate of 0.4 mL/min, using a mobile phase consisting of 0.1% formic acid (A) and acetonitrile (B). The analytes and IS were detected by multiple reaction monitoring (MRM) via negative ion mode with ion transitions of m/z 339.1–m/z 176.8 for aesculin, m/z 176.8–m/z 88.9 for aesculetin, m/z 206.8–m/z 192.1 for fraxetin, m/z 369.1–m/z 206.9 for fraxin, m/z 389.1–m/z 227.0 for polydatin and m/z 415.2–m/z 295.1 for puerarin. This method was validated according to the FDA guidelines and the results met the requirements of analysis. The calibration curves of analytes were linear with correlation coefficients more than 0.9980. The intra- and inter-day precisions were less than 15% and the accuracy was within ±15%. The maximum plasma concentration (Cmax) of aesculin, aesculetin, fraxetin, fraxin and polydatin was 46.75 ± 7.46, 209.9 ± 57.65, 369.7 ± 48.87, 67.04 ± 12.09 and 47.14 ± 12.04 ng/mL, respectively. The time to reach the maximum plasma concentration (Tmax) was 1.32 ± 0.38 h for aesculin, 1.03 ± 0.27 h for aesculetin, 0.94 ± 0.23 h for fraxetin, 0.83 ± 0.18 h for fraxin and 1.15 ± 0.15 h for polydatin. The results indicated that the absorption of aesculin might be slow in beagle dog plasma. This method was successfully applied for pharmacokinetics in beagle dog plasma after oral administration of the extracts of Ledum palustre L. at a dosage of 0.27 g/kg.

scholarly journals Simultaneous Determination of Sulphur metabolites in Arabidopsis thaliana via LC-ESI-MS/MS and 34S-metabolic Labelling

2011 ◽  
Vol 23 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Chin-Lin Hsieh ◽  
Kai-Wun Yeh ◽  
Luit J. De Kok ◽  
Ryh-Nan Pan ◽  
Yueh-Hsiung Kuo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Simultaneous Determination ◽  
Esi Ms ◽  
Metabolic Labelling
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