Systematic assessment of reduced representation bisulfite sequencing to human blood samples: A promising method for large-sample-scale epigenomic studies

2012 ◽  
Vol 157 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Li Wang ◽  
Jihua Sun ◽  
Honglong Wu ◽  
Siyang Liu ◽  
Junwen Wang ◽  
...  
Keyword(s):  
Human Blood ◽  
Bisulfite Sequencing ◽  
Promising Method ◽  
Blood Samples ◽  
Reduced Representation ◽  
Large Sample ◽  
Systematic Assessment ◽  
Reduced Representation Bisulfite Sequencing ◽  
Human Blood Samples

scholarly journals A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Carcinoembryonic Antigen ◽  
Human Blood ◽  
Shell Structure ◽  
Core Shell ◽  
Label Free ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Core Shell Structure ◽  
Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

Borrelia burgdorferi Survival in Human Blood Samples

1988 ◽  
Vol 539 (1 Lyme Disease) ◽  
pp. 444-445 ◽  
Author(s):  
GUY BARANTON ◽  
ISABELLE SAINT-GIRONS
Keyword(s):  
Borrelia Burgdorferi ◽  
Human Blood ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals Evaluation of pesticide residues in human blood samples from Punjab (India)

2015 ◽  
Vol 8 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Jasbir Singh Bedi ◽  
J. P. S. Gill ◽  
P. Kaur ◽  
A. Sharma ◽  
R. S. Aulakh
Keyword(s):  
Human Blood ◽  
Pesticide Residues ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aniruddha Chatterjee ◽  
Euan J. Rodger ◽  
Peter A. Stockwell ◽  
Robert J. Weeks ◽  
Ian M. Morison
Keyword(s):  
Bisulfite Sequencing ◽  
Flow Cell ◽  
Throughput Capacity ◽  
Sequencing Data ◽  
Bioinformatics Pipeline ◽  
Illumina Hiseq ◽  
Reduced Representation ◽  
Reduced Representation Bisulfite Sequencing ◽  
Unique Mapping ◽  
Nucleotide Resolution

Reduced representation bisulfite sequencing (RRBS), which couples bisulfite conversion and next generation sequencing, is an innovative method that specifically enriches genomic regions with a high density of potential methylation sites and enables investigation of DNA methylation at single-nucleotide resolution. Recent advances in the Illumina DNA sample preparation protocol and sequencing technology have vastly improved sequencing throughput capacity. Although the new Illumina technology is now widely used, the unique challenges associated with multiplexed RRBS libraries on this platform have not been previously described. We have made modifications to the RRBS library preparation protocol to sequence multiplexed libraries on a single flow cell lane of the Illumina HiSeq 2000. Furthermore, our analysis incorporates a bioinformatics pipeline specifically designed to process bisulfite-converted sequencing reads and evaluate the output and quality of the sequencing data generated from the multiplexed libraries. We obtained an average of 42 million paired-end reads per sample for each flow-cell lane, with a high unique mapping efficiency to the reference human genome. Here we provide a roadmap of modifications, strategies, and trouble shooting approaches we implemented to optimize sequencing of multiplexed libraries on an a RRBS background.

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