A serological screening assay of human immunodeficiency virus type 1 antibodies based on recombinant protein p24–gp41 as a fusion protein expressed in Escherichia coli

2006 ◽  
Vol 125 (2) ◽  
pp. 295-303
Author(s):  
Fereidoun Mahboudi ◽  
Nickolaeva A. Irina ◽  
Alexander Chevalier ◽  
Ataallah Ghadiri ◽  
Ahmad Adeli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Immunodeficiency Virus ◽  
Recombinant Protein ◽  
Fusion Protein ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Screening Assay ◽  
Serological Screening ◽  
Immunodeficiency Virus

scholarly journals Membrane Relocation but Not Tight Binding of Human Immunodeficiency Virus Type 1 Gag Particles Myristoylated in Escherichia coli

Virology ◽  
2001 ◽  
Vol 283 (2) ◽  
pp. 343-352 ◽  
Author(s):  
Yuko Morikawa ◽  
Ayako Kinoshita ◽  
Toshiyuki Goto ◽  
Hiroshi Tomoda ◽  
Kouichi Sano
Keyword(s):  
Escherichia Coli ◽  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Tight Binding ◽  
Immunodeficiency Virus

scholarly journals Production of Uninfectious Human Immunodeficiency Virus Type 1 Containing Viral Protein R Fused to a Single-Chain Antibody against Viral Integrase

1998 ◽  
Vol 72 (8) ◽  
pp. 6960-6964 ◽  
Author(s):  
Nobuo Okui ◽  
Noriko Kobayashi ◽  
Yoshihiro Kitamura
Keyword(s):  
Human Immunodeficiency Virus ◽  
Fusion Protein ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Protein ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.

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