scholarly journals Discrimination among IgG1-κ monoclonal antibodies produced by two cell lines using charge state distributions in nanoESI-TOF mass spectra

2009 ◽  
Vol 20 (6) ◽  
pp. 1030-1036 ◽  
Author(s):  
Leila Zamani ◽  
Jessica Lindholm ◽  
Leopold L. Ilag ◽  
Sven P. Jacobsson
Keyword(s):  
Monoclonal Antibodies ◽  
Charge State ◽  
Cell Lines ◽  
Mass Spectra ◽  
Charge State Distributions

scholarly journals Measurements of charge state distributions of 0.74 and 1.4  MeV/u heavy ions passing through dilute gases

2017 ◽  
Vol 20 (4) ◽  
Author(s):  
P. Scharrer ◽  
Ch. E. Düllmann ◽  
W. Barth ◽  
J. Khuyagbaatar ◽  
A. Yakushev ◽  
...  
Keyword(s):  
Charge State ◽  
Heavy Ions ◽  
Dilute Gases ◽  
Charge State Distributions

Differential responses of human tumor cell lines to anti-p185HER2 monoclonal antibodies

1993 ◽  
Vol 37 (4) ◽  
pp. 255-263 ◽  
Author(s):  
Gail D. Lewis ◽  
Irene Figari ◽  
Brian Fendly ◽  
Wai Lee Wong ◽  
Paul Carter ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Differential Responses

scholarly journals Functional, Structural, and Distribution Analysis of the Chorionic Gonadotropin Receptor Using Murine Monoclonal Antibodies

2003 ◽  
Vol 88 (11) ◽  
pp. 5537-5546 ◽  
Author(s):  
Ada Funaro ◽  
Anna Sapino ◽  
Bruna Ferranti ◽  
Alberto L. Horenstein ◽  
Isabella Castellano ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
Cell Lines ◽  
Chorionic Gonadotropin ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Signal Pathways ◽  
Distribution Analysis ◽  
Tumor Cell Lines ◽  
Transfected Cells

Abstract LH and human chorionic gonadotropin (hCG) control steroid production and gametogenesis. They also function as growth factors through interaction with a specific receptor that is a member of the seven-transmembrane receptor family coupled via G proteins to signal pathways involving cAMP and phospholipase C/inositol 3 phosphate. For this study, monoclonal antibodies (mAbs) were raised against the human LH receptor (LHR)/hCG receptor (hCGR), using Chinese hamster ovary LHR-transfected cells as the immunogen. Two reagents were then selected on the basis of their ability to recognize the full-length transmembrane re-ceptor expressed both by Chinese hamster ovary LHR-transfected cells and by a limited number of tumor cell lines. One of these mAbs reacts with the LHR/hCGR in tissue sections of both frozen and paraffin-embedded specimens. This unique feature allowed us to map the cytological distribution of LHR/hCGR in human breast tissues at different stages of development in physiological and benign pathological conditions. The same mAb proved to be agonistic: receptor ligation elicits signals that modulate the growth of selected breast tumor cell lines. This observation suggests that the mAb recognizes an epitope that is included in the domain of the receptor involved in the interaction with the natural ligand.

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