scholarly journals Using fluorescence dyes as a tool for analyzing the MALDI process

2008 ◽  
Vol 19 (8) ◽  
pp. 1054-1061 ◽  
Author(s):  
Thorsten W. Jaskolla ◽  
Michael Karas
Keyword(s):  
Maldi Process ◽  
Fluorescence Dyes

scholarly journals A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 151
Author(s):  
Minami Matsuyama ◽  
Yuko Terada ◽  
Toyomi Yamazaki-Ito ◽  
Keisuke Ito
Keyword(s):  
Fluorescent Probe ◽  
Evaluation System ◽  
Food Samples ◽  
Assay System ◽  
Sensory Test ◽  
Chemosensory Receptor ◽  
Luminescence Assay ◽  
Transient Receptor ◽  
Sensory Tests ◽  
Fluorescence Dyes

The quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds, suggesting that the activity of this receptor might be useful as an index for pungency evaluation. Although Ca2+-sensitive fluorescence dyes are commonly used for measuring human TRPV1 (hTRPV1) activity, their application is limited, as foods often contain fluorescent substances that interfere with the fluorescent signals. This study aims to design a new pungency evaluation system using hTRPV1. Instead of employing a fluorescent probe as the Ca2+ indicator, this assay system uses the luminescent protein aequorin. The luminescence assay successfully evaluated the hTRPV1 activity in foods without purification, even for those containing fluorescent substances. The hTRPV1 activity in food samples correlated strongly with the pungency intensity obtained by the human sensory test. This luminescence-based hTRPV1 assay system will be a powerful tool for objectively quantifying the pungency of spicy foods in both laboratory and industrial settings.

Towards the rationalization of the MALDI process: a combined mass spectrometry/solid-state NMR approach

2012 ◽  
Vol 4 (10) ◽  
pp. 3118 ◽  
Author(s):  
Yannis Major ◽  
Hélène Pizzala ◽  
Fabio Ziarelli ◽  
Trang N. T. Phan ◽  
Giulia Mollica ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Solid State ◽  
Solid State Nmr ◽  
Maldi Process

Laser Desorption Mass Spectrometry of Synthetic Multiporphyrin Arrays

1997 ◽  
Vol 01 (01) ◽  
pp. 93-99 ◽  
Author(s):  
DAVID FENYO ◽  
BRIAN T. CHAIT ◽  
THOMAS E. JOHNSON ◽  
JONATHAN S. LINDSEY
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Maldi Ms ◽  
Active Materials ◽  
Laser Desorption Mass Spectrometry ◽  
Desorption Mass Spectrometry ◽  
Laser Illumination ◽  
Powerful Analytical Tool ◽  
Maldi Process ◽  
Insight Into

Laser desorption mass spectrometry and matrix-assisted laser desorption mass spectrometry (MALDI-MS) have been investigated for the analysis of a set of synthetic compounds containing one, two, five, eight or nine porphyrins. Intact singly ionized molecule ions were observed for each compound and the spectra were readily interpretable. The use of a matrix of 4-hydroxy-α-cyano-cinnamic acid greatly diminished the extent of fragmentation. Examination of the resulting mass spectra provides insight into aspects of the MALDI process. The present results show that high molecular weight photochemically active materials that absorb strongly at the wavelength of laser illumination can be analysed effectively and that MALDI-MS is a powerful analytical tool for synthetic chemistry of porphyrin-based molecules with dimensions ranging to 10 nm. The strong molecule ions observed for the largest compounds investigated ( Zn 8-octamer, Zn 9-nonamer) indicates that this method should be applicable to even larger porphyrin arrays.

Spectral studies of multi-branched fluorescence dyes based on triphenylpyridine core

2014 ◽  
Vol 121 ◽  
pp. 355-362 ◽  
Author(s):  
Hai-Ying Wang ◽  
Liang-Feng Chen ◽  
Xiu-Ling Zhu ◽  
Chao Wang ◽  
Yu Wan ◽  
...  
Keyword(s):  
Spectral Studies ◽  
Fluorescence Dyes

Inner Filter Effect on Fluorescence Dyes Spectra in Methanol Solution

2016 ◽  
Vol 675-676 ◽  
pp. 704-707 ◽  
Author(s):  
Saksit Sukprasong ◽  
Yongyut Manjit ◽  
Apichart Limpichaipanit ◽  
Athipong Ngamjarurojana
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Inner Filter Effect ◽  
Fluorescent Dye ◽  
Concentration Effect ◽  
Wavelength Shift ◽  
Filter Effect ◽  
High Concentrations ◽  
Fluorescence Dyes ◽  
Dye Solutions

This research was conducted to study the inner filter effect on the fluorescence spectra of fluorescence dyes. The concentration effect on all of fluorescent dye solutions showed the same trend in terms of changes in fluorescence intensity spectra. At low concentrations, the fluorescence intensity increased when the concentration of fluorescent dye solution increased. However, at high concentrations, the fluorescence intensity decreased when the concentration of fluorescent dye solution increased. Interestingly, the result of fluorescence spectra in dye solutions showed that the fluorescence intensity maxima in all dye solutions were shifted to a higher wavelength (red-shift) when the concentration of fluorescent dye solution increased. The results of concentration effect on fluorescence intensity and wavelength-shift in dyes solution can be explained by inner filter effect on fluorescent dye solutions.

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