scholarly journals MALDI mass spectrometry analysis of high molecular weight proteins from whole bacterial cells: Pretreatment of samples with surfactants

2005 ◽  
Vol 16 (9) ◽  
pp. 1422-1426 ◽  
Author(s):  
Mohammed A. Meetani ◽  
Kent J. Voorhees
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Bacterial Cells ◽  
Spectrometry Analysis ◽  
High Molecular Weight Proteins

Direct Automated MALDI Mass Spectrometry Analysis of Cellular Transporter Function: Inhibition of OATP2B1 Uptake by 294 Drugs

2020 ◽  
Vol 92 (17) ◽  
pp. 11851-11859
Author(s):  
Melissa S. Unger ◽  
Lena Schumacher ◽  
Thomas Enzlein ◽  
David Weigt ◽  
Maciej J. Zamek-Gliszczynski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis

scholarly journals Miniaturized Digestion and Extraction of Surface Proteins from Candida albicans following Treatment with Histatin 5 for Mass Spectrometry Analysis

2016 ◽  
Vol 2016 ◽  
pp. 1-12
Author(s):  
Shirley Fan ◽  
Eduardo B. Moffa ◽  
Yizhi Xiao ◽  
Walter L. Siqueira ◽  
Ken K.-C. Yeung
Keyword(s):  
Mass Spectrometry ◽  
Candida Albicans ◽  
Surface Proteins ◽  
Mass Spectrometry Analysis ◽  
Bacterial Cells ◽  
Intact Cells ◽  
Histatin 5 ◽  
Spectrometry Analysis ◽  
Glass Slides ◽  
Glass Surfaces

A common approach to isolate surface proteins from fungal and bacterial cells is to perform a proteolytic cleavage of proteins on the surface of intact cells suspended in solution. This paper describes miniaturization of this technique, in which cells are adhered on glass surfaces, and all sample treatments are conducted at μL volumes. Specifically, Candida albicans cells were attached onto HSA-coated glass slides. By depositing the appropriate reagent solutions on the adhered cells, we successfully performed cell washing, treatment with antifugal peptide, Histatin 5, and a proteolysis on intact cells with trypsin. The resulting peptides were subsequently analysed by mass spectrometry. In general, the data obtained was similar to that collected with suspended cells in much larger sample volumes. However, our miniaturized workflow offers the benefit of greatly reducing the consumption of cells and reagents.

Advances in structure elucidation of low molecular weight carbohydrates by liquid chromatography-multiple-stage mass spectrometry analysis

2020 ◽  
Vol 1612 ◽  
pp. 460664 ◽  
Author(s):  
A. Martín-Ortiz ◽  
C. Carrero-Carralero ◽  
O. Hernández-Hernández ◽  
R. Lebrón-Aguilar ◽  
F.J. Moreno ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Liquid Chromatography ◽  
Structure Elucidation ◽  
Mass Spectrometry Analysis ◽  
Low Molecular Weight ◽  
Spectrometry Analysis
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