Ancient-DNA reveals an Asian type of Mycobacterium leprae in medieval Scandinavia

2013 ◽  
Vol 40 (1) ◽  
pp. 465-470 ◽  
Author(s):  
Christos Economou ◽  
Anna Kjellström ◽  
Kerstin Lidén ◽  
Ioannis Panagopoulos
Keyword(s):  
Ancient Dna ◽  
Mycobacterium Leprae

scholarly journals Comparison of target enrichment strategies for ancient pathogen DNA

2020 ◽  
Author(s):  
Anja Furtwängler ◽  
Judith Neukamm ◽  
Lisa Böhme ◽  
Ella Reiter ◽  
Melanie Vollstedt ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Treponema Pallidum ◽  
Mycobacterium Leprae ◽  
Target Enrichment ◽  
Hybridization Capture ◽  
Research Outcomes ◽  
Pathogen Dna ◽  
Different Characteristics ◽  
Rna And Dna ◽  
Better Than

AbstractIn ancient DNA research, the degraded nature of the samples generally results in poor yields of highly fragmented DNA, and targeted DNA enrichment is thus required to maximize research outcomes. The three commonly used methods – (1) array-based hybridization capture and in-solution capture using either (2) RNA or (3) DNA baits – have different characteristics that may influence the capture efficiency, specificity, and reproducibility. Here, we compared their performance in enriching pathogen DNA of Mycobacterium leprae and Treponema pallidum of 11 ancient and 19 modern samples. We find that in-solution approaches are the most effective method in ancient and modern samples of both pathogens, and RNA baits usually perform better than DNA baits.Method summaryWe compared three targeted DNA enrichment strategies used in ancient DNA research for the specific enrichment of pathogen DNA regarding their efficiency, specificity, and reproducibility for ancient and modern Mycobacterium leprae and Treponema pallidum samples. Array-based capture and in-solution capture with RNA and DNA baits were all tested in three independent replicates.

scholarly journals Comparison of target enrichment strategies for ancient pathogen DNA

BioTechniques ◽  
2020 ◽  
Vol 69 (6) ◽  
pp. 455-459
Author(s):  
Anja Furtwängler ◽  
Judith Neukamm ◽  
Lisa Böhme ◽  
Ella Reiter ◽  
Melanie Vollstedt ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Treponema Pallidum ◽  
Mycobacterium Leprae ◽  
Capture Efficiency ◽  
Target Enrichment ◽  
Hybridization Capture ◽  
Research Outcomes ◽  
Pathogen Dna ◽  
Different Characteristics ◽  
Better Than

In ancient DNA research, the degraded nature of the samples generally results in poor yields of highly fragmented DNA; targeted DNA enrichment is thus required to maximize research outcomes. The three commonly used methods – array-based hybridization capture and in-solution capture using either RNA or DNA baits – have different characteristics that may influence the capture efficiency, specificity and reproducibility. Here we compare their performance in enriching pathogen DNA of Mycobacterium leprae and Treponema pallidum from 11 ancient and 19 modern samples. We find that in-solution approaches are the most effective method in ancient and modern samples of both pathogens and that RNA baits usually perform better than DNA baits.

Corrigendum to “Ancient-DNA reveals an Asian type of Mycobacterium leprae in medieval Scandinavia” [J. Archaeol. Sci. 40 (1) (2013) 465–470]

2013 ◽  
Vol 40 (6) ◽  
pp. 2867 ◽  
Author(s):  
Christos Economou ◽  
Anna Kjellström ◽  
Kerstin Lidén ◽  
Ioannis Panagopoulos
Keyword(s):  
Ancient Dna ◽  
Mycobacterium Leprae

scholarly journals Non-destructive extraction of DNA from preserved tissues in medical collections

2021 ◽  
Author(s):  
Enrique Rayo ◽  
Giada Ferrari ◽  
Judith Neukamm ◽  
Gülfirde Akgül ◽  
Abagail M. Breidenstein ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Soft Tissues ◽  
Treponema Pallidum ◽  
Mycobacterium Leprae ◽  
Liquid Component ◽  
Human Mitochondrial Dna ◽  
Hybridization Capture ◽  
Specific Source ◽  
Available Technology ◽  
Non Destructive

AbstractMuseum and medically fixed material are valuable samples for the study of historical soft tissues and represent a pathogen-specific source for retrospective molecular investigations. However, current methods for the molecular analysis are inherently destructive, posing a dilemma between performing a study with the available technology thus damaging the sample - or conserving the material for future investigations. Here we present an unprecedented non-destructive alternative that facilitates the genetic analysis of fixed wet tissues while avoiding tissue damage. We extracted DNA from the fixed tissues as well as their embedding fixative solution, to quantify the DNA that was transferred to the liquid component. Our results prove that human ancient DNA can be retrieved from the fixative material of stored medical specimens and provide new options for the sampling of valuable curated collections.Method summaryWe compared the metagenomic content of historical tissues and their embedding liquid to retrieve DNA from the host and specified pathogens based on the diagnosis of the sample. We applied ancient DNA research techniques, including in-solution hybridization capture with DNA baits for human mitochondrial DNA, Mycobacterium tuberculosis, Mycobacterium leprae, and Treponema pallidum.

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