Amplification of Toll-like receptor–mediated signaling through spleen tyrosine kinase in human B-cell activation

2012 ◽  
Vol 129 (6) ◽  
pp. 1594-1601.e2 ◽  
Author(s):  
Shigeru Iwata ◽  
Kunihiro Yamaoka ◽  
Hiroaki Niiro ◽  
Kazuhisa Nakano ◽  
Sheau-Pey Wang ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
B Cell ◽  
Cell Activation ◽  
B Cell Activation ◽  
Toll Like Receptor ◽  
Spleen Tyrosine Kinase ◽  
Human B Cell

HIV-1 induces B-cell activation and class switch recombination via spleen tyrosine kinase and c-Jun N-terminal kinase pathways

AIDS ◽  
2014 ◽  
Vol 28 (16) ◽  
pp. 2365-2374 ◽  
Author(s):  
Ana Judith Perisé-Barrios ◽  
Rafael Correa-Rocha ◽  
Susana Álvarez ◽  
Maria Ángeles Muñoz-Fernandez ◽  
Marjorie Pion
Keyword(s):  
Tyrosine Kinase ◽  
B Cell ◽  
Cell Activation ◽  
Class Switch Recombination ◽  
B Cell Activation ◽  
Spleen Tyrosine Kinase ◽  
Class Switch ◽  
Hiv 1

scholarly journals Development of a Pharmacodynamic Assay Based on PLCγ2 Phosphorylation for Quantifying Spleen Tyrosine Kinase (SYK)–Bruton’s Tyrosine Kinase (BTK) Signaling

2013 ◽  
Vol 18 (8) ◽  
pp. 890-898 ◽  
Author(s):  
Jonathan Hsu ◽  
Jun Zhang ◽  
Chris Kitson ◽  
Seng-Lai Tan ◽  
Satwant Narula ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
B Cell ◽  
Cell Activation ◽  
Cell Receptor ◽  
Bruton’S Tyrosine Kinase ◽  
B Cell Activation ◽  
Spleen Tyrosine Kinase ◽  
Bruton's Tyrosine Kinase ◽  
Surface Receptors ◽  
Activation Assay

Spleen tyrosine kinase (SYK) and Bruton’s tyrosine kinase (BTK) are key mediators in coupling cell surface receptors, such as the B-cell receptor (BCR), to downstream signaling events affecting diverse biological functions. There is therefore tremendous interest in the development of pharmacological inhibitors targeting the SYK-BTK axis for the treatment of inflammatory disorders and hematological malignancies. A good pharmacodynamic (PD) assay, ideally a blood-based assay that measures proximal events, is warranted for evaluation of such inhibitors. In platelets, collagen-induced activation of membrane glycoprotein GPVI is dependent on the SYK-BTK axis. Here, we report the development of a novel immunoassay that uses the dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) to measure GPVI-mediated phosphorylation of phospholipase C γ2 (PLCγ2), a direct substrate of SYK and BTK, in platelets. The assay was validated using SYK or BTK inhibitors and generated IC50 correlated with those from the BCR-induced B-cell activation assay. Furthermore, this assay showed good stability and uniformity over a period of 24 h in different donors. Interestingly, compound IC50 values using blood from patients with rheumatoid arthritis were slightly higher compared with those produced using samples from healthy donors. This novel platelet PLCγ2 phosphorylation-based immunoassay should serve as a promising PD assay for preclinical and clinical development of inhibitors targeting the SYK-BTK axis.

scholarly journals Selective, novel spleen tyrosine kinase (Syk) inhibitors suppress chronic lymphocytic leukemia B-cell activation and migration

Leukemia ◽  
2012 ◽  
Vol 26 (7) ◽  
pp. 1576-1583 ◽  
Author(s):  
J Hoellenriegel ◽  
G P Coffey ◽  
U Sinha ◽  
A Pandey ◽  
M Sivina ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Tyrosine Kinase ◽  
B Cell ◽  
Cell Activation ◽  
Lymphocytic Leukemia ◽  
B Cell Activation ◽  
Spleen Tyrosine Kinase ◽  
And Migration

scholarly journals Amplification of IL-21 signalling pathway through Bruton’s tyrosine kinase in human B cell activation

Rheumatology ◽  
2015 ◽  
Vol 54 (8) ◽  
pp. 1488-1497 ◽  
Author(s):  
Sheau-Pey Wang ◽  
Shigeru Iwata ◽  
Shingo Nakayamada ◽  
Hiroaki Niiro ◽  
Siamak Jabbarzadeh-Tabrizi ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
B Cell ◽  
Cell Activation ◽  
Signalling Pathway ◽  
Bruton’S Tyrosine Kinase ◽  
B Cell Activation ◽  
Bruton's Tyrosine Kinase ◽  
Human B Cell

Inhibitory effect of anti-class II antibodies on human B-cell activation

1988 ◽  
Vol 112 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Yoshiya Tanaka ◽  
Fumihiko Shirakawa ◽  
Toshiyuki Ota ◽  
Hidero Suzuki ◽  
Sumiya Eto ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Activation ◽  
Class Ii ◽  
Inhibitory Effect ◽  
B Cell Activation ◽  
Human B Cell
Sign in / Sign up

Export Citation Format

Share Document