Human recombinant Histamine Releasing Factor (HrHRF) Induced Histamine Release (HR) is Associated with SHIP Knockdown (k/d) in both the Cultured Human Mast Cell (MC) and Basophil Models

2007 ◽  
Vol 119 (1) ◽  
pp. S313
Author(s):  
J.M. Langdon ◽  
J.T. Schroeder ◽  
A.P. Bieneman ◽  
B.M. Vonakis ◽  
S.M. MacDonald
Keyword(s):  
Mast Cell ◽  
Histamine Release ◽  
Human Mast Cell

scholarly journals Histamine-releasing factor/translationally controlled tumor protein (HRF/TCTP)-induced histamine release is enhanced with SHIP-1 knockdown in cultured human mast cell and basophil models

2008 ◽  
Vol 84 (4) ◽  
pp. 1151-1158 ◽  
Author(s):  
Jacqueline M. Langdon ◽  
John T. Schroeder ◽  
Becky M. Vonakis ◽  
Anja P. Bieneman ◽  
Kristin Chichester ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histamine Release ◽  
Human Mast Cell ◽  
Translationally Controlled Tumor Protein

Inhibitory Effects of BiRyuChe-Bang on Mast Cell-Mediated Allergic Reactions and Inflammatory Cytokines Production

2013 ◽  
Vol 41 (06) ◽  
pp. 1267-1282 ◽  
Author(s):  
Phil-Dong Moon ◽  
Il Sang Choi ◽  
Ji-Hyun Go ◽  
Byong-Joo Lee ◽  
Sang Woo Kang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Histamine Release ◽  
Inflammatory Cytokines ◽  
Medical Center ◽  
Human Mast Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Allergic Reactions ◽  
Linalyl Acetate ◽  
Tnf Α

BiRyuChe-bang (BRC) is a Korean prescription medicine, which has been used to treat allergic rhinitis at Kyung Hee Medical Center. In this work, we investigated the effects of BRC on mast cell-mediated allergic reactions and inflammatory cytokines production, and identified the active component of BRC. Histamine release was measured from rat peritoneal mast cells (RPMCs). Ear swelling and passive cutaneous anaphylaxis (PCA) were examined in mouse models. Phorbol 12-myristate 13-acetate (PMA) plus A23187-induced inflammatory cytokines production was measured using enzyme-linked immunosorbent assay. Reverse transcriptase-polymerase chain reaction was used for the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8. Activation of nuclear factor (NF)-κB was analyzed by Western blotting. BRC significantly inhibited the compound 48/80-induced ear swelling response, histamine release from RPMCs, PCA activated by anti-dinitrophenyl IgE, and PMA plus A23187-induced inflammatory cytokines production (p < 0.05). In addition, BRC dose-dependently inhibited the mRNA expressions of TNF-α, IL-6, and IL-8 as well as the activation of NF-κB in a human mast cell line, HMC-1 cells. BRC inhibited the levels of TNF-α and IL-6 in mice induced with PCA. Several components of BRC, such as 1,8-Cineole, Linalool, Linalyl acetate, α-Pinene, and α-Terpineol, significantly inhibited the release of histamine from RPMCs (p < 0.05). Among these components, Linalyl acetate was the most effective for inhibiting histamine release. These results indicate that BRC has a potential regulatory effect on allergic and inflammatory reactions mediated by mast cells.

scholarly journals Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line HMC-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease

2002 ◽  
Vol 70 (7) ◽  
pp. 3944-3947 ◽  
Author(s):  
Yukino Watanabe ◽  
Yuko Todome ◽  
Hisashi Ohkuni ◽  
Shinsaku Sakurada ◽  
Toshio Ishikawa ◽  
...  
Keyword(s):  
Mast Cell ◽  
Histamine Release ◽  
Cell Line ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Human Mast Cell ◽  
Streptococcal Toxic Shock Syndrome ◽  
Streptococcal Pyrogenic Exotoxin B ◽  
Mast Cell Line ◽  
Spe B

ABSTRACT We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained its SCP activity, which was equivalent to or greater than that of the naturally occurring molecule. The secreted rSPE B/SCP induced histamine release and degranulation of the human mast cell line HMC-1. This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome.

COMPARISON OF CUTANEOUS AND IN VITRO HUMAN MAST CELL HISTAMINE RELEASE BY MUSCLE RELAXANTS

1987 ◽  
Vol 66 (Supplement) ◽  
pp. S82
Author(s):  
Carol A. Hirshman ◽  
Chester F. North ◽  
Nancy Kettelkamp
Keyword(s):  
Mast Cell ◽  
Histamine Release ◽  
Muscle Relaxants ◽  
Human Mast Cell

scholarly journals Role of protein kinase A in the inhibition of human mast cell histamine release by β-adrenergic receptor agonists

2002 ◽  
Vol 51 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Toshinobu Kato ◽  
Masahiro Kimata ◽  
Toshikazu Tsuji ◽  
Michitaka Shichijo ◽  
Masayuki Murata ◽  
...  
Keyword(s):  
Mast Cell ◽  
Protein Kinase ◽  
Histamine Release ◽  
Protein Kinase A ◽  
Adrenergic Receptor ◽  
Human Mast Cell ◽  
Receptor Agonists ◽  
Kinase A

scholarly journals Thapsigargin-Stimulated LAD2 Human Mast Cell Line Is a Potent Cellular Adjuvant for the Maturation of Monocyte-Derived Dendritic Cells for Adoptive Cellular Immunotherapy

2021 ◽  
Vol 22 (8) ◽  
pp. 3978
Author(s):  
Pavla Taborska ◽  
Dmitry Stakheev ◽  
Jirina Bartunkova ◽  
Daniel Smrz
Keyword(s):  
Dendritic Cells ◽  
Mast Cell ◽  
Ex Vivo ◽  
Human Leukocyte ◽  
Poly I:C ◽  
Human Mast Cell ◽  
Cellular Immunotherapy ◽  
Adoptive Cellular Immunotherapy ◽  
Serum Free ◽  
Dc Maturation

The preparation of dendritic cells (DCs) for adoptive cellular immunotherapy (ACI) requires the maturation of ex vivo-produced immature(i) DCs. This maturation ensures that the antigen presentation triggers an immune response towards the antigen-expressing cells. Although there is a large number of maturation agents capable of inducing strong DC maturation, there is still only a very limited number of these agents approved for use in the production of DCs for ACI. In seeking novel DC maturation agents, we used differentially activated human mast cell (MC) line LAD2 as a cellular adjuvant to elicit or modulate the maturation of ex vivo-produced monocyte-derived iDCs. We found that co-culture of iDCs with differentially activated LAD2 MCs in serum-containing media significantly modulated polyinosinic:polycytidylic acid (poly I:C)-elicited DC maturation as determined through the surface expression of the maturation markers CD80, CD83, CD86, and human leukocyte antigen(HLA)-DR. Once iDCs were generated in serum-free conditions, they became refractory to the maturation with poly I:C, and the LAD2 MC modulatory potential was minimized. However, the maturation-refractory phenotype of the serum-free generated iDCs was largely overcome by co-culture with thapsigargin-stimulated LAD2 MCs. Our data suggest that differentially stimulated mast cells could be novel and highly potent cellular adjuvants for the maturation of DCs for ACI.

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