Santamarin, a sesquiterpene lactone isolated from Saussurea lappa, represses LPS-induced inflammatory responses via expression of heme oxygenase-1 in murine macrophage cells

2012 ◽  
Vol 13 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Hyun-Gyu Choi ◽  
Dong-Sung Lee ◽  
Bin Li ◽  
Yeon Ho Choi ◽  
Seung-Ho Lee ◽  
...  
Keyword(s):  
Sesquiterpene Lactone ◽  
Heme Oxygenase ◽  
Inflammatory Responses ◽  
Heme Oxygenase 1 ◽  
Murine Macrophage ◽  
Saussurea Lappa ◽  
Macrophage Cells

Celastrol ameliorates HIV-1 Tat-induced inflammatory responses via NF-kappaB and AP-1 inhibition and heme oxygenase-1 induction in astrocytes

2014 ◽  
Vol 280 (1) ◽  
pp. 42-52 ◽  
Author(s):  
Gi Soo Youn ◽  
Dong-Joo Kwon ◽  
Sung Mi Ju ◽  
Hyangshuk Rhim ◽  
Yong Soo Bae ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Inflammatory Responses ◽  
Heme Oxygenase 1 ◽  
Nf Kappab ◽  
Hiv 1

scholarly journals Progesterone-induced activation of membrane-bound progesterone receptors in murine macrophage cells

2014 ◽  
Vol 224 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Jing Lu ◽  
Joshua Reese ◽  
Ying Zhou ◽  
Emmet Hirsch
Keyword(s):  
Progesterone Receptor ◽  
Inflammatory Responses ◽  
Progesterone Receptors ◽  
Murine Macrophage ◽  
Induced Expression ◽  
Receptor Alpha ◽  
Downstream Target ◽  
Macrophage Cells ◽  
Membrane Progesterone Receptor ◽  
Membrane Progesterone Receptor Alpha

Parturition is an inflammatory process mediated to a significant extent by macrophages. Progesterone (P4) maintains uterine quiescence in pregnancy, and a proposed functional withdrawal of P4 classically regulated by nuclear progesterone receptors (nPRs) leads to labor. P4 can affect the functions of macrophages despite the reported lack of expression of nPRs in these immune cells. Therefore, in this study we investigated the effects of the activation of the putative membrane-associated PR on the function of macrophages (a key cell for parturition) and discuss the implications of these findings for pregnancy and parturition. In murine macrophage cells (RAW 264.7), activation of mPRs by P4 modified to be active only extracellularly by conjugation to BSA (P4BSA, 1.0×10−7 mol/l) caused a pro-inflammatory shift in the mRNA expression profile, with significant upregulation of the expression of cyclooxygenase 2 (COX2 (Ptgs2)), Il1B, and Tnf and downregulation of membrane progesterone receptor alpha (Paqr7) and oxytocin receptor (Oxtr). Pretreatment with PD98059, a MEK1/2 inhibitor, significantly reduced P4BSA-induced expression of mRNA of Il1B, Tnf, and Ptgs2. Inhibition of protein kinase A (PKA) by H89 blocked P4BSA-induced expression of Il1B and Tnf mRNA. P4BSA induced rapid phosphorylation of MEK1/2 and CREB (a downstream target of PKA). This phosphorylation was inhibited by pretreatment with PD98059 and H89, respectively, revealing that MEK1/2 and PKA are two of the components involved in mPR signaling. Taken together, these results indicate that changes in membrane progesterone receptor alpha expression and signaling in macrophages are associated with the inflammatory responses; and that these changes might contribute to the functional withdrawal of P4 related to labor.

scholarly journals Deficiency of heme oxygenase-1 impairs renal hemodynamics and exaggerates systemic inflammatory responses to renal ischemia

2007 ◽  
Vol 72 (9) ◽  
pp. 1073-1080 ◽  
Author(s):  
M.J. Tracz ◽  
J.P. Juncos ◽  
A.J. Croatt ◽  
A.W. Ackerman ◽  
J.P. Grande ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Inflammatory Responses ◽  
Renal Hemodynamics ◽  
Renal Ischemia ◽  
Heme Oxygenase 1

scholarly journals Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Mohamed Elfeky ◽  
Ryuji Kaede ◽  
Yuko Okamatsu-Ogura ◽  
Kazuhiro Kimura
Keyword(s):  
Mrna Expression ◽  
Heme Oxygenase ◽  
High Mobility ◽  
Heme Oxygenase 1 ◽  
Amp Kinase ◽  
High Mobility Group Protein ◽  
Macrophage Cells ◽  
Compound C ◽  
Tlr4 Mrna ◽  
Anti Inflammatory

High mobility group protein B1 (HMGB1) is a late inflammatory mediator that exaggerates septic symptoms. Adiponectin, an adipokine, has potent anti-inflammatory properties. However, possible effects of adiponectin on lipopolysaccharide- (LPS-) induced HMGB1 release are unknown. The aim of this study was to investigate effects of full length adiponectin on HMGB1 release in LPS-stimulated RAW 264 macrophage cells. Treatment of the cells with LPS alone significantly induced HMGB1 release associated with HMGB1 translocation from the nucleus to the cytosol. However, prior treatment with adiponectin suppressed LPS-induced HMGB1 release and translocation. The anti-inflammatory cytokine interleukin- (IL-) 10 similarly suppressed LPS-induced HMGB1 release. Adiponectin treatment decreased toll-like receptor 4 (TLR4) mRNA expression and increased heme oxygenase- (HO-) 1 mRNA expression without inducing IL-10 mRNA, while IL-10 treatment decreased TLR2 and HMGB1 mRNA expression and increased the expression of IL-10 and HO-1 mRNA. Treatment with the HO-1 inhibitor ZnPP completely prevented the suppression of HMGB1 release by adiponectin but only partially inhibited that induced by IL-10. Treatment with compound C, an AMP kinase (AMPK) inhibitor, abolished the increase in HO-1 expression and the suppression of HMGB1 release mediated by adiponectin. In conclusion, our results indicate that adiponectin suppresses HMGB1 release by LPS through an AMPK-mediated and HO-1-dependent IL-10-independent pathway.

scholarly journals Biological effects of inhaled hydraulic fracturing sand dust. III. Cytotoxicity and pro-inflammatory responses in cultured murine macrophage cells

2020 ◽  
Vol 408 ◽  
pp. 115281 ◽  
Author(s):  
Nicole S. Olgun ◽  
Anna M. Morris ◽  
Aleksandr B. Stefaniak ◽  
Lauren N. Bowers ◽  
Alycia K. Knepp ◽  
...  
Keyword(s):  
Hydraulic Fracturing ◽  
Inflammatory Responses ◽  
Biological Effects ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Sand Dust
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