Effect of horse gram lectin (Dolichos biflorus agglutinin) on degranulation of mast cells and basophils of atopic subjects: Identification as an allergen

2006 ◽  
Vol 6 (11) ◽  
pp. 1714-1722 ◽  
Author(s):  
Siddanakoppalu N. Pramod ◽  
Thirumalai P. Krishnakantha ◽  
Yeldur P. Venkatesh
Keyword(s):  
Mast Cells ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin ◽  
Horse Gram

scholarly journals Dolichos biflorus agglutinin (DBA) reacts selectively with mast cells in human connective tissues.

1988 ◽  
Vol 36 (10) ◽  
pp. 1231-1237 ◽  
Author(s):  
M Hormia ◽  
A L Kariniemi ◽  
L Laitinen ◽  
I Virtanen
Keyword(s):  
Endothelial Cells ◽  
Mast Cells ◽  
Blood Group ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Connective Tissues ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin ◽  
Blood Group A ◽  
Group A

Dolichos biflorus agglutinin (DBA) binds to N-acetyl-D-galactosamine (GalNAc) residues in glycoconjugates and agglutinates erythrocytes carrying blood group antigen A. In cryostat sections of various tissues from blood group-specified humans, fluorochrome-coupled DBA bound preferentially to fusiform connective tissue cells and to certain epithelial cells. The connective tissue cells were identified as mast cells by their typical metachromasia in consecutive staining with toluidine blue. Double labeling with DBA and conjugated avidin revealed two distinct populations of mast cells. In several tissues the DBA-reactive cells likewise displayed uniform avidin reactivity. In intestinal mucosa, however, morphologically distinct DBA-binding mast cells were found, which were labeled with the avidin conjugates only in specially fixed paraffin sections. DBA did not bind to vascular endothelial cells, which could be identified by double staining with antibodies to factor VIII-related antigen. Labeling with Helix pomatia agglutinin (HPA), another blood group A-reactive lectin, resulted in distinct blood group-dependent fluorescence of the endothelia. Sophora japonica agglutinin (SJA), a blood group B-reactive lectin, labeled vascular endothelial cells in tissues from blood group A, AB, and B donors. HPA and SJA reacted with small mast cells in the gastrointestinal mucosa but failed to label large mast cells in any of the tissues. These results indicate that the blood group reactivity of lectins, as determined by erythroagglutination, is not necessarily consistent with their reactivity with blood group determinants in tissue sections. Moreover, DBA conjugates appear to be a reliable probe for detection of mast cells in various human connective tissues.

Structural Studies on a Binding Site for Dolichos biflorus Agglutinin in the Small Intestine of the Mouse1

1991 ◽  
Vol 109 (1) ◽  
pp. 178-183 ◽  
Author(s):  
Yuko Kamada ◽  
Hisako Muramatsu ◽  
Yuko Arita ◽  
Toshihide Yamada ◽  
Takashi Muramatsu
Keyword(s):  
Small Intestine ◽  
Binding Site ◽  
Structural Studies ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin

Dolichos biflorus agglutinin binding in hereditary bowel cancer

Pathology ◽  
1994 ◽  
Vol 26 (2) ◽  
pp. 110-114 ◽  
Author(s):  
J.R. Jass ◽  
L.J. Allison ◽  
S.M. Stewart ◽  
M.R. Lane
Keyword(s):  
Bowel Cancer ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin

Receptors for Dolichos biflorus Agglutinin on Embryonal Carcinoma Cells1

1981 ◽  
Vol 89 (2) ◽  
pp. 473-481 ◽  
Author(s):  
Takashi MURAMATSU ◽  
Hisako MURAMATSU ◽  
Masayuki OZAWA
Keyword(s):  
Embryonal Carcinoma ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin
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