The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo

2005 ◽  
Vol 5 (5) ◽  
pp. 903-916 ◽  
Author(s):  
Fumihide Takano ◽  
Nobuo Yahagi ◽  
Remiko Yahagi ◽  
Satoko Takada ◽  
Masaya Yamaguchi ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Ex Vivo ◽  
Cytokine Response ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Th2 Cytokine ◽  
Culture Filtrates ◽  
Th1 And Th2

scholarly journals Combination Effects of Herbs in a Multi-herbal Formula: Expression of Juzen-taiho-to's Immuno-modulatory Activity on the Intestinal Immune System

2004 ◽  
Vol 1 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Hiroaki Kiyohara ◽  
Tsukasa Matsumoto ◽  
Haruki Yamada
Keyword(s):  
Ex Vivo ◽  
Clinical Effects ◽  
Combination Effect ◽  
Immune Functions ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Combination Effects ◽  
Intestinal Immune System ◽  
Single Formula

Herbal formulas of traditional Japanese (Kampo), Chinese and Korean medicines usually comprise multiple herbs in a single formula. These medicines are expected to show their clinical effects by chemical, pharmacological and pharmaceutical combination effects of multi-herbs. However, little effort has been made so far to scientifically clarify the nature of such combination effects. Interestingly, for example, though a Kampo medicine Juzen-taiho-to (Shi-Quan-Da-Bu-Tang in Chinese) stimulates the immune functions of Peyer's patch cells, none of its single component herbs shows such activity. We thus examined the combination effect of herbs in the Juzen-taiho-to formula for the expression of its immuno-stimulating activity. Juzen-taiho-to, a composite formula of 10 herbs, has been generally considered to comprise two kinds of basic formula, each of which consists of four different herbs in addition to two others. The combinations of herbs based on these two basic formulas were evaluated for their stimulating activities on cytokine production from murine Peyer's patch cells bothin vitroandex vivo. Combined decoction of six among 10 herbs in Juzen-taiho-to is crucial for the expression of its stimulating activity on Peyer's patch cells. 3D-HPLC analysis of the ingredients in the fractions from the combined decoctions indicated that, in addition to quantitative changes of ingredients, alterations occur in their chemical composition by decoction of different herbs. The stimulating activity of Juzen-taiho-to on Peyer's patch cells results from the combination effect of its six essential component herbs. This combination effect is based on physicochemical interactions among the ingredients of the component herbs.

scholarly journals Mice Are Protected from Helicobacter pylori Infection by Nasal Immunization with AttenuatedSalmonella typhimurium phoPc Expressing Urease A and B Subunits

1998 ◽  
Vol 66 (2) ◽  
pp. 581-586 ◽  
Author(s):  
Irène E. Corthésy-Theulaz ◽  
Sally Hopkins ◽  
Daniel Bachmann ◽  
Pierre F. Saldinger ◽  
Nadine Porta ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Expression System ◽  
Mucosal Vaccine ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Cervical Lymph Nodes ◽  
B Subunits ◽  
H Pylori ◽  
Th1 And Th2

ABSTRACT Live Salmonella typhimurium phoPc bacteria were tested as mucosal vaccine vectors to deliver Helicobacter pylori antigens. The genes encoding the A and B subunits ofH. pylori urease were introduced into S. typhimurium phoPc and expressed under the control of a constitutive tac promoter (tac-ureAB) or a two-phase T7 expression system (cT7-ureAB). Both recombinant Salmonella strains expressed the two urease subunits in vitro and were used to nasally immunize BALB/c mice. The plasmid carrying cT7-ureAB was stably inherited by bacteria growing or persisting in the spleen, lungs, mesenteric or cervical lymph nodes, and Peyer’s patches of immunized mice, while the plasmid carrying tac-ureAB was rapidly lost. Spleen and Peyer’s patch CD4+ lymphocytes from mice immunized with S. typhimurium phoPc cT7-ureAB proliferated in vitro in response to urease, whereas cells from mice given S. typhimurium phoPc alone did not. Splenic CD4+ cells from mice immunized with phoPc cT7-ureAB secreted gamma interferon and interleukin 10, while Peyer’s patch CD4+ cells did not secrete either cytokine. Specific H. pylori anti-urease immunoglobulin G1 (IgG1) and IgG2A antibodies were detected following immunization, confirming that both Th1- and Th2-type immune responses were generated by the live vaccine. Sixty percent of the mice (9 of 15) immunized with S. typhimurium phoPc cT7-ureAB were found to be resistant to infection by H. pylori, while all mice immunized with phoPc tac-ureAB (15 of 15) or phoPc (15 of 15) were infected. Our data demonstrate that H. pylori urease delivered nasally by using a vaccine strain of S. typhimurium can trigger Th1- and Th2-type responses and induce protective immunity againstHelicobacter infection.

scholarly journals Peyer's Patch Dendritic Cells Process Viral Antigen from Apoptotic Epithelial Cells in the Intestine of Reovirus-infected Mice

2004 ◽  
Vol 200 (2) ◽  
pp. 235-245 ◽  
Author(s):  
Marina N. Fleeton ◽  
Nikhat Contractor ◽  
Francisco Leon ◽  
J. Denise Wetzel ◽  
Terence S. Dermody ◽  
...  
Keyword(s):  
T Cells ◽  
Epithelial Cells ◽  
Cd4 T Cells ◽  
Cell Protein ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Antigen Uptake ◽  
Cross Presentation

We explored the role of Peyer's patch (PP) dendritic cell (DC) populations in the induction of immune responses to reovirus strain type 1 Lang (T1L). Immunofluorescence staining revealed the presence of T1L structural (σ1) and nonstructural (σNS) proteins in PPs of T1L-infected mice. Cells in the follicle-associated epithelium contained both σ1 and σNS, indicating productive viral replication. In contrast, σ1, but not σNS, was detected in the subepithelial dome (SED) in association with CD11c+/CD8α−/CD11blo DCs, suggesting antigen uptake by these DCs in the absence of infection. Consistent with this possibility, PP DCs purified from infected mice contained σ1, but not σNS, and PP DCs from uninfected mice could not be productively infected in vitro. Furthermore, σ1 protein in the SED was associated with fragmented DNA by terminal deoxy-UTP nick-end labeling staining, activated caspase-3, and the epithelial cell protein cytokeratin, suggesting that DCs capture T1L antigen from infected apoptotic epithelial cells. Finally, PP DCs from infected mice activated T1L-primed CD4+ T cells in vitro. These studies show that CD8α−/CD11blo DCs in the PP SED process T1L antigen from infected apoptotic epithelial cells for presentation to CD4+ T cells, and therefore demonstrate the cross-presentation of virally infected cells by DCs in vivo during a natural viral infection.

scholarly journals Recombinant Human Growth Hormone Modulates Th1 and Th2 Cytokine Response in Burned Mice

1998 ◽  
Vol 228 (1) ◽  
pp. 106-111 ◽  
Author(s):  
Kazuya Takagi ◽  
Fujiyo Suzuki ◽  
Robert E. Barrow ◽  
Steven E. Wolf ◽  
David N. Herndon
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Recombinant Human Growth Hormone ◽  
Human Growth ◽  
Cytokine Response ◽  
Th2 Cytokine ◽  
Th1 And Th2

Th1 and Th2 Cytokine Response Patterns in Leukocyte Cultures of Patients with Urinary Bladder, Renal Cell and Prostate Carcinomas

Tumor Biology ◽  
1998 ◽  
Vol 19 (6) ◽  
pp. 470-476 ◽  
Author(s):  
U. Elsässer-Beile ◽  
N. Kölble ◽  
T. Grussenmeyer ◽  
W. Schultze-Seemann ◽  
U. Wetterauer ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Renal Cell ◽  
Cytokine Response ◽  
Response Patterns ◽  
Th2 Cytokine ◽  
Prostate Carcinomas ◽  
Th1 And Th2

scholarly journals Pre-Existing Adenovirus Immunity Modifies a Complex Mixed Th1 and Th2 Cytokine Response to an Ad5/HIV-1 Vaccine Candidate in Humans

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18526 ◽  
Author(s):  
Samuel O. Pine ◽  
James G. Kublin ◽  
Scott M. Hammer ◽  
Joleen Borgerding ◽  
Yunda Huang ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Cytokine Response ◽  
Th2 Cytokine ◽  
Hiv 1 ◽  
Th1 And Th2

Antibiotics modulate the stimulated cytokine response to endotoxin in a human ex vivo, in vitro model

2006 ◽  
Vol 50 (9) ◽  
pp. 1103-1110 ◽  
Author(s):  
S. Ziegeler ◽  
A. Raddatz ◽  
G. Hoff ◽  
H. Buchinger ◽  
I. Bauer ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ex Vivo ◽  
Cytokine Response ◽  
Vitro Model
Sign in / Sign up

Export Citation Format

Share Document