Optimization of simultaneous saccharification and fermentation for the production of ethanol from sweet sorghum (Sorghum bicolor) bagasse using response surface methodology

2013 ◽  
Vol 42 ◽  
pp. 280-291 ◽  
Author(s):  
Lijun Wang ◽  
Zhenglin Luo ◽  
Abolghasem Shahbazi
Keyword(s):  
Response Surface Methodology ◽  
Sorghum Bicolor ◽  
Response Surface ◽  
Sweet Sorghum ◽  
Simultaneous Saccharification And Fermentation ◽  
Simultaneous Saccharification

scholarly journals Response Surface ‎Methodology for Optimization of Ethanol ‎Production from Cocoa Waste

2020 ◽  
Vol 1 (1) ◽  
pp. 7-12
Keyword(s):  
Response Surface Methodology ◽  
Ethanol Production ◽  
Response Surface ◽  
Simultaneous Saccharification And Fermentation ◽  
Inoculum Size ◽  
Fermented Food ◽  
Optimum Conditions ◽  
Anova Analysis ◽  
Full Factorial ◽  
Simultaneous Saccharification

Cocoa waste (CW) is an inexpensive agro-industrial by-product that is available in large quantities in tropical countries such as Malaysia. The rate of ethanol production can be affected by different parameters involved during fermentation. In this study, response surface methodology (RSM) with the full factorial design was used to obtain optimum conditions for bioethanol production using CW as the substrate. The simultaneous saccharification and fermentation (SSF) performed with an isolated microorganism from locally fermented food tapai ubi and tapai pulut. The effect of four independent variables temperature, CW concentration, inoculum size, and pH was investigated. In the optimized condition temperature of 31.7°C, pH 6.0, inoculum size 10.5%, and CW concentration 0.3 g/L, the highest ethanol production was 9.5 ± 1.1. ANOVA analysis revealed that temperature and CW concentration had the most significant effects on ethanol production. In addition, ethanol production was increased in the highest level of pH and inoculum size. Therefore it can be concluded that ethanol production increased from 6.2 ± 0.8 g/L to 9.5 ± 1.1 g/L after optimization.

scholarly journals PRODUKSI BIOETANOL dari BATANG Sorghum bicolor (L.) Moench dengan Saccharomyces cerevisiae dan KONSORSIUM S. cerevisiae-Pichia stipitis

2016 ◽  
Vol 16 (2) ◽  
Author(s):  
Ellina S Pandebesie ◽  
Audiananti Meganandi Kartini
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sorghum Bicolor ◽  
Response Surface ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Sorghum Bicolor L ◽  
Simultaneous Saccharification

Indonesia merupakan penghasil biomassa lignoselulosa residu pertanian yang cukup melimpah, salah satunya adalah limbah pertanian sorgum yang belum memiliki nilai ekonomis berupa batang Sorgum (Sorghum bicolor (L.) Moench). Total gula dalam nira sorgum hampir setara dengan nira tebu. Berdasarkan kandungan total gula yang cukup tinggi tersebut, limbah pertanian sorgum berupa batang sorgum beserta kandungan niranya  adalah salah satu potensi sumber bioetanol yang menjanjikan.Penelitian ini dilakukan untuk menentukan konsentrasi substrat optimum dan proses fermentasi batang sorgum dengan menggunakan metode  Simultaneous Saccharification and Fermentation (SSF). Pretreatment  yang akan digunakan adalah dengan metode  fisik dan kimia. Batang sorgum yang  telah  menjadi  bubuk  dibuat dalam  konsentrasi 25 gram (5%) dan 50 gram (10%), kemudian dilakukan penambahan aquades hingga 500 ml. Substrat diperlakukan dengan pretreatment pencacahan, pengeringan dan penggilingan, kemudian dilakukan penambahan H2SO4 0,25% dan dipanaskan pada suhu 121o C selama 10 menit. Metode hidrolisis pada penelitian ini menggunakan metode hidrolisis enzimatik, yaitu dengan memanfaatkan kapang T. viride dan A. niger. Sampel yang telah ditambahkan T. viride dan A. nigerkemudian ditambahkan S. cerevisiae CC 3012 dan konsorsium S. cerevisiae CC 3012-P. stipitis untuk proses fermentasi. Data yang  diperoleh  dalam  penelitian  ini  yaitu  kadar  lignin,  selulosa, hemiselulosa, gula reduksi, pH, C, N, P dan bioetanol. Analisis menggunakan Response Surface digunakan untuk mengambil kesimpulan dan mengetahui nilai optimum terhadap berbagai perlakuan substrat yang digunakan. Hasil penelitian ini menunjukkan bahwa pemanfaatan batang sorgum berpotensi  rendah  sebagai  bahan  baku  bioetanol  karena menghasilkan kadar gula reduksi dan etanol yang tergolong rendah. Hasil  etanol  tertinggi dihasilkan pada substrat 50 gram (10%), yaitu sebesar 2.1% dengan  penambahan konsorsium S. cerevisiae CC 3012-P. stipitis 10 % selama 24 jam fermentasi.

scholarly journals Bioethanol production from cocoa waste by locally isolated microorganism using response surface methodology

2018 ◽  
Vol 3 (4) ◽  
pp. 160-166
Author(s):  
Shahabaldin Rezania ◽  
Shaza Eva Mohamad ◽  
Adibah Yahya ◽  
Madihah Md Salleh
Keyword(s):  
Response Surface Methodology ◽  
Ethanol Production ◽  
Response Surface ◽  
Simultaneous Saccharification And Fermentation ◽  
Inoculum Size ◽  
Fermented Food ◽  
Anova Analysis ◽  
Lignocellulosic Substrate ◽  
Central Composite ◽  
Simultaneous Saccharification

The rate of ethanol production can be affected by different parameters that involved during fermentation. In this study, acid treated cocoa waste (CW) was used as a lignocellulosic substrate for ethanol production in the simultaneous saccharification and fermentation (SSF) using microorganism isolated from locally fermented food tapai ubi and tapai pulut. For optimization, the experiments were carried out using response surface methodology (RSM). The effect of four independent variables temperature, CW concentration, inoculum size and pH during fermentation was investigated. A central composite design (CCD) was used to evaluate the effect and interactions of the parameters. ANOVA analysis revealed that pH and inoculum size had the most significant effects on the ethanol production. The optimized condition for the ethanol production was at temperature 31.7°C, pH 6.0, inoculum size 10.5% and CW concentration 0.3g/L while after optimization, ethanol podcution increased from 6.2±0.8g/L to 9.5±1.1g/L.

Optimization of saccharification of sweet sorghum bagasse using response surface methodology

2013 ◽  
Vol 44 ◽  
pp. 211-219 ◽  
Author(s):  
Jitendra K. Saini ◽  
Rahul K. Anurag ◽  
Arti Arya ◽  
B.K. Kumbhar ◽  
Lakshmi Tewari
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sweet Sorghum ◽  
Sweet Sorghum Bagasse ◽  
Sorghum Bagasse

Efficient l-lactic acid production from sweet sorghum bagasse by open simultaneous saccharification and fermentation

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35771-35777 ◽  
Author(s):  
Y. Wang ◽  
M. Wang ◽  
D. Cai ◽  
B. Wang ◽  
Z. Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Sweet Sorghum ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Sweet Sorghum Bagasse ◽  
Sorghum Bagasse ◽  
Ssf Process ◽  
Simultaneous Saccharification

An open SSF process using B. coagulans LA1507 introduces an effective way to produce l-lactic acid from abundant SSB.

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