Antifungal activity in vitro of Flourensia spp. extracts on Alternaria sp., Rhizoctonia solani, and Fusarium oxysporum

2007 ◽  
Vol 25 (2) ◽  
pp. 111-116 ◽  
Author(s):  
D. Jasso de Rodríguez ◽  
D. Hernández-Castillo ◽  
J.L. Angulo-Sánchez ◽  
R. Rodríguez-García ◽  
J.A. Villarreal Quintanilla ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Alternaria Sp

scholarly journals Chromatographic profiles of antifungal exo- and endometabolites of Bacillus velezensis

2021 ◽  
Vol 2(26) ◽  
pp. 191-199
Author(s):  
T.M. Sidorova ◽  
◽  
A.M. Asaturova ◽  
V.V. Allakhverdyan ◽  
◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Active Metabolites ◽  
Fungicidal Action ◽  
Antifungal Metabolites ◽  
Alternaria Sp ◽  
Tlc Analysis

The antifungal activity of the Bacillus bacteria is based on their ability to produce metabolites. Therefore, when selecting a strain that produces an effective biofungicide, it is necessary to assess the metabolism of bacteria. The aim of this work is to isolate exo- and endometabolites of the promising B. velezensis BZR 336g and B. velezensis BZR 517 strains and assess their antifungal activity. Studies were carried out in 2020–2021. The object of the study is a liquid culture of the B. velezensis BZR 336g and B. velezensis BZR 517 strains. Methods of liquid extraction, ascending thin layer chromatography (TLC), bioautography with a test-culture of Fusarium oxysporum var. orthoceras and Alternaria sp. fungi were used to analyze metabolites. The ability of the strains to accumulate a complex of active metabolites showing antifungal effect from fungistatic to fungicidal action was revealed. On the bioautogram of exometabolites, we found two most pronounced zones (Rf 0.18 and 0.29) of Fusarium oxysporum var. orthoceras BZR P1 growth inhibition (fungicide). Zones with Rf 0.58 for B. velezensis BZR 336g and Rf 0.70 for B. velezensis BZR 517 correspond to the test fungus growth retardation (fungistatic). Significant suppression of Alternaria sp. BZR P8 growth was also observed in two zones (Rf 0.18 and 0.29). The use of surfactin, iturin A, fengycin (Sigma-Aldrich®) in the TLC analysis made it possible to detect similar lipopeptides in the composition of metabolite complexes produced by the studied bacteria. It should be noted that the studied strains differed both in their ability to produce metabolites of different structure (can be found when analyzing chromatograms under ultraviolet light) and in their effect on phytopathogenic fungi in vitro. This may indicate possible differences in the mechanism of antagonistic activity of bacteria against phytopathogenic fungi. Thus, B. velezensis BZR 336g and B. velezensis BZR 517 produce a significant set of antifungal metabolites and can be used as strains to produce effective biofungicides.

scholarly journals In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina.

2017 ◽  
Vol 6 (9) ◽  
pp. 1676 ◽  
Author(s):  
Ramaraju Cherkupally ◽  
Srinivasa Reddy Kota ◽  
Hindumathi Amballa ◽  
Bhumi Narasimha Reddy
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Aloe Vera ◽  
Datura Stramonium ◽  
Macrophomina Phaseolina ◽  
Calotropis Procera ◽  
Fungitoxic Effect

The antifungal activity of aqueous extracts of nine plants viz, Azadirachta indica, Parthenium hysterophorus, Momordica charantia, Allium sativum, Eucalyptus globules, Calotropis procera, Aloe vera, Beta vulgaris and Datura stramonium were assessed in vitro against Fusarium oxysporum f. sp. melongenae, Rhizoctonia solani and Macrophomina phaseolina, the soil borne phytopathogens. The assessment of fungitoxic effect was carried out by using three different concentrations i.e., 5, 10 and 20% against the test fungi, in terms of percentage of mycelial growth inhibition. The extract of A. sativum completely inhibited the mycelial growth of M. phaseolina at all the concentrations. The extracts of D. stramonium and E. globulus inhibited the mycelial growth of R. solani of 72%, and 70.7% respectively at 20% concentration, that of A. sativum, E. globulus and D. stramonium exhibited inhibition percentage of 63.3%, 61.8% and 61.1% respectively at 20% concentration on Fusarium oxysporum f. sp. melongenae. The application of plant extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.

Antifungal activity in vitro of Rhus muelleri against Fusarium oxysporum f. sp. lycopersici

2015 ◽  
Vol 75 ◽  
pp. 150-158 ◽  
Author(s):  
D. Jasso de Rodríguez ◽  
F.A. Trejo-González ◽  
R. Rodríguez-García ◽  
M.L.V. Díaz-Jimenez ◽  
A. Sáenz-Galindo ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum

Synthesis and Antifungal Activities of Some 2,6-Bis-(Un)Substituted Phenoxymethylpyridines

2010 ◽  
Vol 65 (7-8) ◽  
pp. 433-436
Author(s):  
Hui Xu ◽  
Huan Qu
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Fusarium Graminearum ◽  
Broad Spectrum ◽  
Alternaria Alternata ◽  
Phytopathogenic Fungi ◽  
Alternaria Brassicae ◽  
Antifungal Activities

Several 2,6-bis-(un)substituted phenoxymethylpyridines were synthesized and evaluated in vitro against Fusarium graminearum, Helminthosporium sorokinianum, Alternaria brassicae, Alternaria alternata, and Fusarium oxysporum f. sp. vasinfectum. Among all derivatives, compound 3 a exhibited a broad-spectrum antifungal activity against the five phytopathogenic fungi.

Antifungal activity of ajoene, a constituent of garlic (Allium sativum)

1990 ◽  
Vol 68 (6) ◽  
pp. 1354-1356 ◽  
Author(s):  
U. P. Singh ◽  
V. N. Pandey ◽  
K. G. Wagner ◽  
K. P. Singh
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Spore Germination ◽  
Allium Sativum ◽  
Alternaria Solani ◽  
Field Conditions ◽  
Fusarium Udum ◽  
Alternaria Tenuissima ◽  
Fusarium Semitectum ◽  
Alternaria Sp

Ajoene, a compound derived from garlic (Allium sativum L.), inhibited spore germination of some fungi, namely, Alternaria solani, Alternaria tenuissima, Alternaria triticina, Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium lini, Fusarium oxysporum, Fusarium semitectum, and Fusarium udum, which cause serious diseases in some important crop plants in India. The compound was very effective in checking spore germination at a concentration of 25 μg/mL in some of the above fungi and, in most cases, there was 100% inhibition of germination at 100 μg/mL. It is quite likely that the compound may be useful in controlling disease(s) under field conditions.

scholarly journals Fungistasis del aceite esencial extraído de una población de Tagetes lucida de Hidalgo, México

2018 ◽  
Vol 9 (2) ◽  
pp. 329-341
Author(s):  
Edgar López López ◽  
Margarita Gisela Peña Ortega ◽  
María Teresa Beryl Colinas León ◽  
Francisco Díaz Cedillo ◽  
Miguel Ángel Serrato Cruz
Keyword(s):  
Aspergillus Niger ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Penicillium Janthinellum

La especie T. lucida conocida como ‘pericón’, es un recurso natural de México cuyo aceite esencial tiene propiedades antifúngicas, aunque no en todos los hongos fitopatógenos se ha explorado el efecto que puede ocasionar esta sustancia vegetal. De una población recolectada en Atotonilco el Grande, Hidalgo, México se obtuvo aceite esencial por hidrodestilación con rendimiento de 0.2%; mediante la técnica CG-EM se identificaron ocho componentes diferentes en el aceite, pero los abundantes fueron: estragol (48%) y anetol (35%). También se evaluó la actividad antimicótica in vitro del aceite esencial contra Aspergillus niger, Fusarium oxysporum, Penicillium janthinellum y Rhizoctonia solani mediante la técnica de difusión en agar, realizando dos experimentos: el primero ensayando concentraciones de 0, 0.1 y 1% y en el segundo 0, 2 y 3%. La concentración de 1% redujo el crecimiento micelial 46% para F. oxysporum, 39% en R. solani, 21% en A. niger y 16% en P. janthinellum; sin embargo, en concentraciones altas de aceite, como 3%, solo en R. solani se observó la mayor la reducción del crecimiento micelial (72%).

scholarly journals Antagonismo de Trichoderma harzianum contra la fusariosis del garbanzo y su efecto biofertilizante

2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Baja California ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).

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