Intraperitoneal immunization with Urease loaded N -trimethyl Chitosan nanoparticles elicits high protection against Brucella melitensis and Brucella abortus infections

2018 ◽  
Vol 199 ◽  
pp. 53-60 ◽  
Author(s):  
Morteza Abkar ◽  
Mahdi Fasihi-Ramandi ◽  
Hamid Kooshki ◽  
Abbas Sahebghadam Lotfi
Keyword(s):  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Chitosan Nanoparticles ◽  
Trimethyl Chitosan

Identification, geographic distribution and risk factors of Brucella abortus and Brucella melitensis infection in cattle in Algeria

2021 ◽  
Vol 254 ◽  
pp. 109004
Author(s):  
Nedjma Lounes ◽  
Falk Melzer ◽  
Ashraf E. Sayour ◽  
Hassiba Tali Maamar ◽  
Kheira Rahal ◽  
...  
Keyword(s):  
Risk Factors ◽  
Geographic Distribution ◽  
Brucella Abortus ◽  
Brucella Melitensis

scholarly journals Development and Evaluation of Diclofenac Sodium Loaded-N-Trimethyl Chitosan Nanoparticles for Ophthalmic Use

2015 ◽  
Vol 16 (5) ◽  
pp. 1013-1024 ◽  
Author(s):  
Rathapon Asasutjarit ◽  
Thitaree Theerachayanan ◽  
Prartana Kewsuwan ◽  
Sukitaya Veeranodha ◽  
Asira Fuongfuchat ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Chitosan Nanoparticles ◽  
Trimethyl Chitosan

scholarly journals Deletion of znuA Virulence Factor Attenuates Brucella abortus and Confers Protection against Wild-Type Challenge

2006 ◽  
Vol 74 (7) ◽  
pp. 3874-3879 ◽  
Author(s):  
Xinghong Yang ◽  
Todd Becker ◽  
Nancy Walters ◽  
David W. Pascual
Keyword(s):  
Brucella Abortus ◽  
Deletion Mutant ◽  
Pasteurella Multocida ◽  
Brucella Melitensis ◽  
Normal Growth ◽  
Wild Type ◽  
Minimal Growth ◽  
M Genome ◽  
S19 Vaccine ◽  
Knockout Mutation

ABSTRACT znuA is known to be an important factor for survival and normal growth under low Zn2+ concentrations for Escherichia coli, Haemophilus spp., Neisseria gonorrhoeae, and Pasteurella multocida. We hypothesized that the znuA gene present in Brucella melitensis 16 M would be similar to znuA in B. abortus and questioned whether it may also be an important factor for growth and virulence of Brucella abortus. Using the B. melitensis 16 M genome sequence, primers were designed to construct a B. abortus deletion mutant. A znuA knockout mutation in B. abortus 2308 (ΔznuA) was constructed and found to be lethal in low-Zn2+ medium. When used to infect macrophages, ΔznuA B. abortus showed minimal growth. Further study with ΔznuA B. abortus showed that its virulence in BALB/c mice was attenuated, and most of the bacteria were cleared from the spleen within 8 weeks. Protection studies confirmed the ΔznuA mutant as a potential live vaccine, since protection against wild-type B. abortus 2308 challenge was as effective as that obtained with the RB51 or S19 vaccine strain.

Evaluation of neuropeptide loaded trimethyl chitosan nanoparticles for nose to brain delivery

2013 ◽  
Vol 61 ◽  
pp. 189-195 ◽  
Author(s):  
Manoj Kumar ◽  
Ravi Shankar Pandey ◽  
Kartik Chandra Patra ◽  
Sunil Kumar Jain ◽  
Muarai Lal Soni ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Brain Delivery ◽  
Trimethyl Chitosan

Drug permeability and mucoadhesion properties of thiolated trimethyl chitosan nanoparticles in oral insulin delivery

Biomaterials ◽  
2009 ◽  
Vol 30 (29) ◽  
pp. 5691-5700 ◽  
Author(s):  
Lichen Yin ◽  
Jieying Ding ◽  
Chunbai He ◽  
Liming Cui ◽  
Cui Tang ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Insulin Delivery ◽  
Oral Insulin ◽  
Drug Permeability ◽  
Trimethyl Chitosan

PMMA-N,N,N-trimethyl chitosan nanoparticles for fabrication of antibacterial natural rubber latex gloves

2014 ◽  
Vol 109 ◽  
pp. 1-6 ◽  
Author(s):  
Thanida Arpornwichanop ◽  
Duangporn Polpanich ◽  
Raweewan Thiramanas ◽  
Teeraporn Suteewong ◽  
Pramuan Tangboriboonrat
Keyword(s):  
Natural Rubber ◽  
Chitosan Nanoparticles ◽  
Natural Rubber Latex ◽  
Rubber Latex ◽  
Trimethyl Chitosan ◽  
Latex Gloves

scholarly journals Evaluation of Protection Afforded by Brucella abortus and Brucella melitensis Unmarked Deletion Mutants Exhibiting Different Rates of Clearance in BALB/c Mice

2006 ◽  
Vol 74 (7) ◽  
pp. 4048-4057 ◽  
Author(s):  
M. M. Kahl-McDonagh ◽  
T. A. Ficht
Keyword(s):  
Mouse Model ◽  
Brucella Abortus ◽  
Protective Immunity ◽  
Brucella Melitensis ◽  
Deletion Mutants ◽  
Heterologous Challenge ◽  
Current Vaccine ◽  
Protection Against Infection ◽  
Ideal Vaccine

ABSTRACT Research for novel Brucella vaccines has focused upon the development of live vaccine strains, which have proven more efficacious than killed or subunit vaccines. In an effort to develop improved vaccines, signature-tagged mutant banks were screened to identify mutants attenuated for survival. Mutants selected from these screens exhibited various degrees of attenuation characterized by the rate of clearance, ranging from a failure to grow in macrophages after 24 h of infection to a failure to persist in the mouse model beyond 8 weeks. Ideal vaccine candidates should be safe to the host, while evoking protective immunity. In the present work, we constructed unmarked deletion mutants of three gene candidates, manBA, virB2, and asp24, in both Brucella abortus and Brucella melitensis. The Δasp24 mutants, which persist for extended periods in vivo, are superior to current vaccine strains and to other deletion strains tested in the mouse model against homologous challenge infection after 12, 16, and 20 weeks postvaccination. The Δasp24 mutants also display superior protection compared to ΔmanBA and ΔvirB2 mutants against heterologous challenge in mice. From this study, a direct association between protection against infection and cytokine response was not apparent between all vaccine groups and, therefore, correlates of protective immunity will need to be considered further. A distinct correlation between persistence of the vaccine strain and protection against infection was corroborated.

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