Safety and immunogenicity of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the E2 protein of classical swine fever virus in pigs

2016 ◽  
Vol 174 ◽  
pp. 63-71 ◽  
Author(s):  
Jian-Lin Lei ◽  
Shui-Li Xia ◽  
Yimin Wang ◽  
Mingliang Du ◽  
Guang-Tao Xiang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Pseudorabies Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Virus Variant ◽  
E2 Protein

scholarly journals Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant

2015 ◽  
Vol 81 (11) ◽  
pp. 3745-3752 ◽  
Author(s):  
Yi-Gang Xu ◽  
Xue-Ting Guan ◽  
Zhong-Mei Liu ◽  
Chang-Yong Tian ◽  
Li-Chun Cui
Keyword(s):  
Immune Responses ◽  
Lactobacillus Plantarum ◽  
Classical Swine Fever Virus ◽  
Oral Vaccine ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
E2 Protein ◽  
Content Type ◽  
Cellular Immune

ABSTRACTClassical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFV-neutralizing antibodies and cytotoxic T lymphocyte (CTL) activitiesin vivo. Thymosin α-1 (Tα1), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineeredLactobacillus plantarumbacteria expressing CSFV E2 protein alone (L. plantarum/pYG-E2) and in combination with Tα1 (L. plantarum/pYG-E2-Tα1) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinantL. plantarum/pYG-E2 andL. plantarum/pYG-E2-Tα1 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (P< 0.05) in the levels of immune responses were observed betweenL. plantarum/pYG-E2-Tα1 andL. plantarum/pYG-E2, suggesting a better immunogenicity ofL. plantarum/pYG-E2-Tα1 as a result of the Tα1 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinantLactobacillusmicroecological agent expressing CSFV E2 protein combined with Tα1 as an adjuvant provides a promising strategy for vaccine development against CSFV.

Stability of classical swine fever virus and pseudorabies virus in animal feed ingredients exposed to transpacific shipping conditions

2020 ◽  
Vol 67 (4) ◽  
pp. 1623-1632 ◽  
Author(s):  
Ana M. M. Stoian ◽  
Vlad Petrovan ◽  
Laura A. Constance ◽  
Matthew Olcha ◽  
Scott Dee ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Pseudorabies Virus ◽  
Animal Feed ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Feed Ingredients

Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization

Vaccine ◽  
2016 ◽  
Vol 34 (33) ◽  
pp. 3723-3730 ◽  
Author(s):  
Xun Liao ◽  
Zuohuan Wang ◽  
Tong Cao ◽  
Chao Tong ◽  
Shichao Geng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Neutralization ◽  
E2 Protein ◽  
Antigenic Region

Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli

2014 ◽  
Vol 159 (12) ◽  
pp. 3219-3230 ◽  
Author(s):  
Myunghwan Jung ◽  
Yun Ji Shin ◽  
Ju Kim ◽  
Seung-Bin Cha ◽  
Won-Jung Lee ◽  
...  
Keyword(s):  
Immune Responses ◽  
Oral Administration ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein ◽  
Rice Calli

Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus

2013 ◽  
Vol 113 (3) ◽  
pp. 483-490 ◽  
Author(s):  
Jinn-Chin Yiu ◽  
Cheng-Wei Liu ◽  
Ruei-Yuan Su ◽  
Wan-Jun Lai ◽  
Menq-Jiau Tseng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Vaccine Candidate ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

scholarly journals Generation and Immunogenicity of a Recombinant Pseudorabies Virus Co-Expressing Classical Swine Fever Virus E2 Protein and Porcine Circovirus Type 2 Capsid Protein Based on Fosmid Library Platform

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 279
Author(s):  
Muhammad Abid ◽  
Teshale Teklue ◽  
Yongfeng Li ◽  
Hongxia Wu ◽  
Tao Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Pseudorabies Virus ◽  
Classical Swine Fever ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Fosmid Library ◽  
Fever Virus ◽  
Porcine Circovirus ◽  
Live Virus

Pseudorabies (PR), classical swine fever (CSF), and porcine circovirus type 2 (PCV2)-associated disease (PCVAD) are economically important infectious diseases of pigs. Co-infections of these diseases often occur in the field, posing significant threat to the swine industry worldwide. gE/gI/TK-gene-deleted vaccines are safe and capable of providing full protection against PR. Classical swine fever virus (CSFV) E2 glycoprotein is mainly used in the development of CSF vaccines. PCV2 capsid (Cap) protein is the major antigen targeted for developing PCV2 subunit vaccines. Multivalent vaccines, and especially virus-vectored vaccines expressing foreign proteins, are attractive strategies to fight co-infections for various swine diseases. The gene-deleted pseudorabies virus (PRV) can be used to develop promising and economical multivalent live virus-vectored vaccines. Herein, we constructed a gE/gI/TK-gene-deleted PRV co-expressing E2 of CSFV and Cap of PCV2 by fosmid library platform established for PRV, and the expression of E2 and Cap proteins was confirmed using immunofluorescence assay and western blotting. The recombinant virus propagated in porcine kidney 15 (PK-15) cells for 20 passages was genetically stable. The evaluation results in rabbits and pigs demonstrate that rPRVTJ-delgE/gI/TK-E2-Cap elicited detectable anti-PRV antibodies, but not anti-PCV2 or anti-CSFV antibodies. These findings provide insights that rPRVTJ-delgE/gI/TK-E2-Cap needs to be optimally engineered as a promising trivalent vaccine candidate against PRV, PCV2 and CSFV co-infections in future.

scholarly journals Generation and Efficacy Evaluation of a Recombinant Pseudorabies Virus Variant Expressing the E2 Protein of Classical Swine Fever Virus in Pigs

2015 ◽  
Vol 22 (10) ◽  
pp. 1121-1129 ◽  
Author(s):  
Yimin Wang ◽  
Jin Yuan ◽  
Xin Cong ◽  
Hua-Yang Qin ◽  
Chun-Hua Wang ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Pseudorabies Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Complete Protection ◽  
Efficacy Evaluation ◽  
Lethal Challenge ◽  
Recombinant Pseudorabies Virus

ABSTRACTClassical swine fever (CSF) is an economically important infectious disease of pigs caused by classical swine fever virus (CSFV). Pseudorabies (PR), which is caused by pseudorabies virus (PRV), is another important infectious disease of pigs and other animals. Coinfections of pigs with PRV and CSFV occur occasionally in the field. The modified live vaccine Bartha-K61 strain has played an important role in the control of PR in many countries, including China. Since late 2011, however, increasing PR outbreaks caused by an emerging PRV variant have been reported in Bartha-K61-vaccinated swine populations on many farms in China. Previously, we generated a gE/gI-deleted PRV (rPRVTJ-delgE) based on this PRV variant, which was shown to be safe and can provide rapid and complete protection against lethal challenge with the PRV variant in pigs. Here, we generated a new recombinant PRV variant expressing the E2 gene of CSFV (rPRVTJ-delgE/gI-E2) and evaluated its immunogenicity and efficacy in pigs. The results showed that rPRVTJ-delgE/gI-E2 was safe for pigs, induced detectable anti-PRV and anti-CSFV neutralizing antibodies, and provided complete protection against the lethal challenge with either the PRV TJ strain or the CSFV Shimen strain. The data indicate that rPRVTJ-delgE/gI-E2 is a promising candidate bivalent vaccine against PRV and CSFV coinfections.

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