Fetal calf serum-primed dendritic cells induce a strong anti-fetal calf serum immune response and diabetes protection in the non-obese diabetic mouse

2007 ◽  
Vol 108 (2) ◽  
pp. 129-136 ◽  
Author(s):  
N. Kadri ◽  
N. Potiron ◽  
M. Ouary ◽  
D. Jegou ◽  
E. Gouin ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cells ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Diabetic Mouse

scholarly journals Fetal Calf Serum-Free Generation of Functionally Active Murine Dendritic Cells Suitable for In Vivo Therapeutic Approaches

2000 ◽  
Vol 114 (1) ◽  
pp. 142-148 ◽  
Author(s):  
Gabriele Müller ◽  
Anke Müller ◽  
Helmut Jonuleit ◽  
Kerstin Steinbrink ◽  
Claudia Szalma ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Therapeutic Approaches ◽  
Serum Free ◽  
Free Generation

Immune Response to Fetal Calf Serum by Two Adenosine Deaminase-Deficient Patients After T Cell Gene Therapy

2002 ◽  
Vol 13 (13) ◽  
pp. 1605-1610 ◽  
Author(s):  
Laura Tuschong ◽  
Sherry L. Soenen ◽  
R. Michael Blaese ◽  
Fabio Candotti ◽  
Linda Mesler Muul
Keyword(s):  
Gene Therapy ◽  
Immune Response ◽  
T Cell ◽  
Adenosine Deaminase ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Cell Gene

Generation of Dendritic Cells from Human Chronic Myelomonocytic Leukemia Cells in Fetal Calf Serum-Free Medium

2000 ◽  
Vol 38 (5-6) ◽  
pp. 577-586 ◽  
Author(s):  
Leopold Oehler ◽  
Andrea Berer ◽  
Felix Keil ◽  
Georg Weinländer ◽  
Margit König ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Chronic Myelomonocytic Leukemia ◽  
Leukemia Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Myelomonocytic Leukemia

scholarly journals Specific peptide-mediated immunity against established melanoma tumors with dendritic cells requires IL-2 and fetal calf serum-free cell culture

2002 ◽  
Vol 32 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Andreas O. Eggert ◽  
Jürgen C. Becker ◽  
Michael Ammon ◽  
Alexander D. McLellan ◽  
German Renner ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dendritic Cells ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Free Cell ◽  
Serum Free ◽  
Specific Peptide

scholarly journals The role of fetal calf serum in the primary immune response in vitro.

1977 ◽  
Vol 145 (4) ◽  
pp. 1029-1038 ◽  
Author(s):  
H G Opitz ◽  
U Opitz ◽  
H Lemke ◽  
G Hewlett ◽  
W Schreml ◽  
...  
Keyword(s):  
Immune Response ◽  
Culture Medium ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Primary Immune Response ◽  
Spleen Cells ◽  
Humoral Immune ◽  
Serum Component ◽  
Immune Response In Vitro

The mode of action of 2-mercaptoethanol (2-ME) on the primary immune response in vitro was investigated. Fetal calf serum (FCS) was preincubated with 2-ME and lyophilized to remove free 2-ME. This 2-ME-treated FCS was able to substitute the function of adherent cells in the primary immune response against sheep red blood cells (SRBC) in vitro; Fractionation of 2-tme-treated FCS on a Sephadex G-100 column showed that 2-ME acted on a high molecular serum component which after activation, could substitute for macrophages. In order to obtain a humoral immune response against SRBC in vitro, spleen cells require selected FCS. These "good" sera could be distinguished from "deficient" sera by their higher content of this 2-ME-activated factor. The height of the in vitro immune response to SRBC was dependent on the amount of activated factor added to the culture medium. FCS normally required in the culture medium could be completely replaced by the factor-containing fraction without deleterious effect on the culture medium. The factor should be added to the spleen cells during the first 24 h of culture and remain there for 72 h in order to obtain an optimal immune response. The factor could be partially absorbed by spleen cells but not by SRBC. The relationship between macrophage, 2-ME, and FCS in eliciting an in vitro primary immune response is discussed.

Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions

1997 ◽  
Vol 27 (12) ◽  
pp. 3135-3142 ◽  
Author(s):  
Helmut Jonuleit ◽  
Ulrich Kühn ◽  
Gabriele Müller ◽  
Kerstin Steinbrink ◽  
Lydia Paragnik ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Inflammatory Cytokines ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Serum Free ◽  
Serum Free Conditions ◽  
Pro Inflammatory Cytokines

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

Fish kidney cell line in response to heat shock

1992 ◽  
Vol 50 (1) ◽  
pp. 704-705
Author(s):  
Li-Chu Tung ◽  
Yung-Reui Chen ◽  
Shiu-Nan Chen ◽  
Guang-Hsiung Kuo
Keyword(s):  
Heat Shock ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Ultrastructural Changes ◽  
Heat Shock Treatment ◽  
Acanthopagrus Schlegeli ◽  
Cacodylate Buffer ◽  
Fish Kidney

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

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