Determination of CD59 protein in normal human serum by enzyme immunoassay, using octyl-glucoside detergent to release glycosyl-phosphatidylinositol-CD59 from lipid complex

2003 ◽  
Vol 90 (2-3) ◽  
pp. 209-213 ◽  
Author(s):  
A.P.G Landi ◽  
A.B Wilson ◽  
A Davies ◽  
P.J Lachmann ◽  
V.P.L Ferriani ◽  
...  
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
Normal Human Serum ◽  
Lipid Complex ◽  
Glycosyl Phosphatidylinositol ◽  
Octyl Glucoside ◽  
Normal Human

Standardization of immunochemical determinations of serum albumin.

1981 ◽  
Vol 27 (5) ◽  
pp. 736-738 ◽  
Author(s):  
J W Keyser ◽  
R Fifield ◽  
G L Watkins
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Normal Human Serum ◽  
Reference Standard ◽  
Protein Nitrogen ◽  
Critical Comparison ◽  
Normal Human ◽  
Theoretical Results

Abstract When we compared four commercially available preparations of human serum albumin with the human serum standard IFCC 74/1 by radial immunodiffusion, by immunoprecipitation turbidimetry, by laser nephelometry, and by "rocket" immunoelectrophoresis, three of the preparations gave almost "theoretical" results with the immunoprecipitation turbidimetric method. Results by the other three methods tended inconsistently to be low. Four pools of normal human serum were also analyzed for albumin by these four immunochemical methods, again with IFCC 74/1 as the reference standard. The results were virtually identical with those obtained by fractionation with 1.8 mol/L sodium sulfate and determination of protein nitrogen in the filtrate. We suggest that a combination of (a) fractionation of a pool of normal human serum in this way and (b) critical comparison with selected commercial preparations of human serum albumin will permit standardization of the serum albumin determination.

scholarly journals The determination of haptoglobins in normal human serum

1961 ◽  
Vol 78 (4) ◽  
pp. 723-728 ◽  
Author(s):  
H. Smith ◽  
J. A. Owen
Keyword(s):  
Human Serum ◽  
Normal Human Serum ◽  
Normal Human
Sign in / Sign up

Export Citation Format

Share Document