Generation of di-lithiated peptide ions from multiply protonated peptides via ion/ion reactions

2007 ◽  
Vol 267 (1-3) ◽  
pp. 183-189 ◽  
Author(s):  
Brittany D.M. Hodges ◽  
Xiaorong Liang ◽  
Scott A. McLuckey
Keyword(s):  
Peptide Ions ◽  
Protonated Peptides

scholarly journals Chimera Spectrum Diagnostics for Peptides Using Two-Dimensional Partial Covariance Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3728
Author(s):  
Taran Driver ◽  
Nikhil Bachhawat ◽  
Leszek J. Frasinski ◽  
Jonathan P. Marangos ◽  
Vitali Averbukh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Peptide Sequence ◽  
Two Dimensional ◽  
Peptide Ions ◽  
Finite Mass ◽  
Protein Database ◽  
Spectra Analysis ◽  
Fragment Ions ◽  
Single Precursor ◽  
The Common

The rate of successful identification of peptide sequences by tandem mass spectrometry (MS/MS) is adversely affected by the common occurrence of co-isolation and co-fragmentation of two or more isobaric or isomeric parent ions. This results in so-called `chimera spectra’, which feature peaks of the fragment ions from more than a single precursor ion. The totality of the fragment ion peaks in chimera spectra cannot be assigned to a single peptide sequence, which contradicts a fundamental assumption of the standard automated MS/MS spectra analysis tools, such as protein database search engines. This calls for a diagnostic method able to identify chimera spectra to single out the cases where this assumption is not valid. Here, we demonstrate that, within the recently developed two-dimensional partial covariance mass spectrometry (2D-PC-MS), it is possible to reliably identify chimera spectra directly from the two-dimensional fragment ion spectrum, irrespective of whether the co-isolated peptide ions are isobaric up to a finite mass accuracy or isomeric. We introduce ‘3-57 chimera tag’ technique for chimera spectrum diagnostics based on 2D-PC-MS and perform numerical simulations to examine its efficiency. We experimentally demonstrate the detection of a mixture of two isomeric parent ions, even under conditions when one isomeric peptide is at one five-hundredth of the molar concentration of the second isomer.

scholarly journals Mass spectral and theoretical investigations of the transient proton-bound dimers on the cleavage processes of the peptide GHK and its analogues

RSC Advances ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 4077-4086
Author(s):  
Jinhu Wang ◽  
Cheng Wang ◽  
Han Zhang ◽  
Yang Liu ◽  
Tiesheng Shi
Keyword(s):  
Mass Spectrometry ◽  
Theoretical Calculations ◽  
Mass Spectral ◽  
Protonated Peptides ◽  
Fragmentation Mechanisms ◽  
Theoretical Investigations

Fragmentation mechanisms of the singly protonated peptides GHK, GHKH and HGHK have been investigated by mass spectrometry and theoretical calculations.

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview

2008 ◽  
Vol 10 (8) ◽  
pp. 1079-1090 ◽  
Author(s):  
Julia Laskin ◽  
Peng Wang ◽  
Omar Hadjar
Keyword(s):  
Peptide Ions ◽  
Soft Landing ◽  
Self Assembled Monolayer ◽  
Self Assembled

scholarly journals Tandem MS Analysis of Selenamide-Derivatized Peptide Ions

2011 ◽  
Vol 22 (9) ◽  
pp. 1610-1621 ◽  
Author(s):  
Yun Zhang ◽  
Hao Zhang ◽  
Weidong Cui ◽  
Hao Chen
Keyword(s):  
Tandem Ms ◽  
Peptide Ions ◽  
Ms Analysis

scholarly journals Surface-induced dissociation of peptide ions in Fourier-transform mass spectrometry

1990 ◽  
Vol 1 (5) ◽  
pp. 413-416 ◽  
Author(s):  
Evan R. Williams ◽  
Kent D. Henry ◽  
Fred W. McLafferty ◽  
Jeffrey Shabanowitz ◽  
Donald F. Hunt
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Fourier Transform Mass Spectrometry ◽  
Peptide Ions ◽  
Surface Induced Dissociation

scholarly journals Spectroscopic evidence for mobilization of amide position protons during CID of model peptide ions

2009 ◽  
Vol 20 (10) ◽  
pp. 1841-1845 ◽  
Author(s):  
Samuel Molesworth ◽  
Christopher M. Leavitt ◽  
Gary S. Groenewold ◽  
Jos Oomens ◽  
Jeffrey D. Steill ◽  
...  
Keyword(s):  
Peptide Ions ◽  
Model Peptide ◽  
Spectroscopic Evidence

Electron Transfer/Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations

2019 ◽  
Vol 30 (9) ◽  
pp. 1578-1585 ◽  
Author(s):  
Martin Penkert ◽  
Anett Hauser ◽  
Robert Harmel ◽  
Dorothea Fiedler ◽  
Christian P. R. Hackenberger ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Peptide Ions ◽  
Doubly Charged
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