High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections

2007 ◽  
Vol 260 (2-3) ◽  
pp. 203-211 ◽  
Author(s):  
A.F. Maarten Altelaar ◽  
Ioana M. Taban ◽  
Liam A. McDonnell ◽  
Peter D.E.M. Verhaert ◽  
Robert P.J. de Lange ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Length Scales ◽  
Tissue Sections ◽  
Pituitary Tissue ◽  
Maldi Imaging Mass Spectrometry

Ionic Matrix for Enhanced MALDI Imaging Mass Spectrometry for Identification of Phospholipids in Mouse Liver and Cerebellum Tissue Sections

2010 ◽  
Vol 82 (21) ◽  
pp. 8800-8806 ◽  
Author(s):  
Kamlesh Shrivas ◽  
Takahiro Hayasaka ◽  
Naoko Goto-Inoue ◽  
Yuki Sugiura ◽  
Nobuhiro Zaima ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Liver ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Tissue Sections ◽  
Maldi Imaging Mass Spectrometry ◽  
Ionic Matrix

Orthogonal organic and aqueous-based washes of tissue sections to enhance protein sensitivity by MALDI imaging mass spectrometry

2013 ◽  
Vol 48 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Aurélien Thomas ◽  
Nathan Heath Patterson ◽  
Jade Laveaux Charbonneau ◽  
Pierre Chaurand
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Tissue Sections ◽  
Maldi Imaging Mass Spectrometry

scholarly journals High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids

2014 ◽  
Vol 25 (8) ◽  
pp. 1394-1403 ◽  
Author(s):  
David M. G. Anderson ◽  
Zsolt Ablonczy ◽  
Yiannis Koutalos ◽  
Jeffrey Spraggins ◽  
Rosalie K. Crouch ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Imaging Mass Spectrometry ◽  
Maldi Imaging ◽  
Retinal Tissue ◽  
Tissue Lipids ◽  
Maldi Imaging Mass Spectrometry

On the Importance of Mathematical Methods for Analysis of MALDI-Imaging Mass Spectrometry Data

2012 ◽  
Vol 9 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Dennis Trede ◽  
Jan Hendrik Kobarg ◽  
Janina Oetjen ◽  
Herbert Thiele ◽  
Peter Maass ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Mass Spectrometry Data ◽  
Maldi Imaging ◽  
Label Free ◽  
Imaging Data ◽  
Mathematical Methods ◽  
Data Set ◽  
Tissue Sections ◽  
Maldi Imaging Mass Spectrometry

Summary In the last decade, matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS), also called as MALDI-imaging, has proven its potential in proteomics and was successfully applied to various types of biomedical problems, in particular to histopathological label-free analysis of tissue sections. In histopathology, MALDI-imaging is used as a general analytic tool revealing the functional proteomic structure of tissue sections, and as a discovery tool for detecting new biomarkers discriminating a region annotated by an experienced histologist, in particular, for cancer studies.A typical MALDI-imaging data set contains 108 to 109 intensity values occupying more than 1 GB. Analysis and interpretation of such huge amount of data is a mathematically, statistically and computationally challenging problem. In this paper we overview some computational methods for analysis of MALDI-imaging data sets. We discuss the importance of data preprocessing, which typically includes normalization, baseline removal and peak picking, and hightlight the importance of image denoising when visualizing IMS data.

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