scholarly journals Diagnosis of Mycoplasma pneumoniae infection in children by using serology and polymerase chain reaction in community-acquired lower respiratory tract infections

2016 ◽  
Vol 45 ◽  
pp. 348
Author(s):  
S. Kumar ◽  
I.B. Garg ◽  
G.R. Sethi ◽  
S.R. Saigal ◽  
S. Kumar
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Tract ◽  
Mycoplasma Pneumoniae ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Lower Respiratory Tract Infections ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections ◽  
Mycoplasma Pneumoniae Infection

scholarly journals Prevalence of Human Bocavirus in Children Under 5 Years with Upper and Lower Respiratory Infections in Southeastern Iran

2022 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Ali Hosseininasab ◽  
Hamidreza Mollaie ◽  
Zainab Karimi
Keyword(s):  
Polymerase Chain Reaction ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Respiratory Infections ◽  
Human Bocavirus ◽  
Lower Respiratory Tract Infections ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections ◽  
Children Under 5

Background: Human Bocavirus (HBoV) is a parvovirus associated with mild to severe upper and lower respiratory tract infections in children. Objectives: This study aimed to detect the virus in the nasopharynx of children under 5-year-old with respiratory infection by polymerase chain reaction (PCR). Methods: Two hundred samples were taken from children referred to pediatric clinics in Kerman, southeastern Iran, with respiratory infections and were positive for virus by PCR. Next, the positive samples were genotyped by real-time PCR. Results: Out of 200 samples, 13 (6.5%) were positive for the Bocavirus gene, and all positive samples were infected by HBoV-1. We observed that 116 patients were male, and there was no difference in the prevalence of the virus based on gender (P = 0.345). The prevalence was significantly higher in infants under 10 months old (P = 0.049). Infection by Bucavirus virus was significantly correlated with symptoms, such as fever (P = 0.035, r = 0.7), otitis media (P = 0.013, r = 0.8), diarrhea, nausea, and vomiting (P = 0.001, r = 0.4). Conclusions: According to our findings, HBoV could be one of the causes of infections in the respiratory system of children, and the only type in the studied region is HBoV-1.

scholarly journals Comparison of PCR for Sputum Samples Obtained by Induced Cough and Serological Tests for Diagnosis of Mycoplasma pneumoniae Infection in Children

2006 ◽  
Vol 13 (6) ◽  
pp. 708-710 ◽  
Author(s):  
Tsutomu Yamazaki ◽  
Mitsuo Narita ◽  
Nozomu Sasaki ◽  
Tsuyoshi Kenri ◽  
Yoshichika Arakawa ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Sensitivity And Specificity ◽  
Mycoplasma Pneumoniae ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Immunoglobulin M ◽  
Lower Respiratory Tract Infections ◽  
Serological Tests ◽  
Tract Infections ◽  
Mycoplasma Pneumoniae Infection

ABSTRACT Passive agglutination (PA) and immunoglobulin M (IgM), IgA, and IgG enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of Mycoplasma pneumoniae were compared with PCR testing of sputum samples obtained from children with lower respiratory tract infections. The sensitivity and specificity of PA were 80.3% and 92.3% at a titer of 1:80. ELISA was found to be less sensitive than PA.

Sign in / Sign up

Export Citation Format

Share Document