scholarly journals Prenatal exposure to sodium valproate alters androgen receptor expression in the developing cerebellum in a region and age specific manner in male and female rats

2016 ◽  
Vol 53 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Miguel Perez‐Pouchoulen ◽  
Marta Miquel ◽  
Paul Saft ◽  
Brenda Brug ◽  
Rebeca Toledo ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Sodium Valproate ◽  
Prenatal Exposure ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Specific Manner ◽  
Developing Cerebellum

scholarly journals Prenatal Exposure to Lamotrigine: Effects on Postnatal Development and Behaviour in Rat Offspring

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sekar Sathiya ◽  
Murugan Ganesh ◽  
Periyathambi Kalaivani ◽  
Vijayan Ranju ◽  
Srinivasan Janani ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Prenatal Exposure ◽  
Clinical Signs ◽  
Female Offspring ◽  
Receptor Expression ◽  
Female Rats ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Male And Female ◽  
Male And Female Rats

Use of antiepileptic drugs (AEDs) in pregnancy warrants various side effects and also deleterious effects on fetal development. The present study was carried out to assess the effects of prenatal exposure to lamotrigine (LTG) on postnatal development and behavioural alterations of offspring. Adult male and female Sprague Dawley rats weighing 150–180 g b. wt. were allowed to copulate and pregnancy was confirmed by vaginal cytology. Pregnant rats were treated with LTG (11.5, 23, and 46 mg/kg, p.o) from gestational day 3 (GND 3) and this treatment continued till postnatal day 11 (PND 11). Offspring were separated from their dam on day 21 following parturition. LTG, at 46 mg/kg, p.o, produced severe clinical signs of toxicity leading to death of dam between GND 15 and 17. LTG, at 11.5 and 23 mg/kg, p.o, showed significant alterations in offspring’s incisors eruption and vaginal opening when compared to age matched controls. LTG (23 mg/kg, p.o) exposed female offspring expressed hyperactive behaviour and decreased GABA-A receptor expression when compared to control rats. These results reveal that prenatal exposure to LTG may impart differential postnatal behavioural alterations between male and female rats which paves way for further investigations.

Nuclear and cytosolic androgen receptor levels in the limbic brain of neonatal male and female rats

1985 ◽  
Vol 23 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Michael J. Meaney ◽  
David H. Aitken ◽  
Lisa K. Jensen ◽  
Marilyn Y. McGinnis ◽  
Bruce S. McEwen
Keyword(s):  
Androgen Receptor ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats

Sex-steroid receptors in the diethylnitrosamine model of hepatocarcinogenesis: modifications by gonadal ablation and steroid replacement therapy

1989 ◽  
Vol 3 (3) ◽  
pp. 229-237 ◽  
Author(s):  
S. Tejura ◽  
G. R. Rodgers ◽  
M. H. Dunion ◽  
M. A. Parsons ◽  
J. C. E. Underwood ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Androgen Receptors ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Female Rats ◽  
Male Rats ◽  
Ovariectomized Rats ◽  
Sex Steroid ◽  
Oestrogen Receptors ◽  
Liver Tumours

ABSTRACT The results of this study confirm our previous report of increased androgen receptor expression in livers of female SUAH Wistar rats during development of liver tumours induced by diethylnitrosamine (DENA). In adult female rats not treated with DENA, removal of the ovary increased liver androgen receptor levels but testosterone did not further enhance the androgen receptor status of ovariectomized rats. In normal adult males the testis and/or testosterone maintained high levels of androgen receptors but oestrogen reduced them in castrated rats. Oestrogen receptor levels were not significantly changed in either males or females by gonadectomy. Treatment of female rats with DENA for 10 and 16 weeks increased liver androgen receptors but oestrogen receptors were only reduced by 16 weeks of DENA treatment, whether the rats were intact or ovariectomized. Concentrations of liver androgen receptors were increased in intact and castrated male rats by 10 and 16 weeks of DENA treatment, an increase not seen in the previous experiments. Oestrogen appeared to inhibit both the increases in liver androgen receptor expression and liver tumour development in rats treated with the weakly carcinogenic dose of 10 weeks of DENA. However, the full carcinogenic dose of 16 weeks of DENA increased liver androgen receptors and decreased oestrogen receptors in female rats regardless of sex-steroid status. Development of malignant hepatocellular carcinoma (HCC) was associated with both an increase in liver androgen receptors and a decrease in oestrogen receptors. Maintenance of relatively high levels of liver oestrogen receptors appeared to protect the liver against development of HCC.

Immunohistochemical localization and immunoblotting of androgen receptor in spinal neurons of male and female rats

1996 ◽  
Vol 134 (5) ◽  
pp. 626-632 ◽  
Author(s):  
S Lumbroso ◽  
F Sandillon ◽  
V Georget ◽  
JM Lobaccaro ◽  
AO Brinkmann ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Androgen Receptor ◽  
Muscular Atrophy ◽  
Polyclonal Antibodies ◽  
Immunohistochemical Localization ◽  
Female Rats ◽  
Spinal Neurons ◽  
Adult Rats ◽  
Male And Female ◽  
Male And Female Rats

Lumbroso S. Sandillon F, Georget V. Lobaccaro JM, Brinkmann AO, Privat A, Sultan C. Immunohistochemical localization and immunoblotting of androgen receptor in spinal neurons of male and female rats. Eur J Endocrinol 1996:134;626–32. ISSN 0804–4643 Androgen activity in the central nervous system, as in other tissues, is mediated by the androgen receptor. We performed the precise localization of the androgen receptor in spinal cord of male and female adult rats by immunohistochemistry using polyclonal antibodies. Light microscopy indicated immunoreactivity in the anterior horn with a strong staining in motoneurons, but staining was also observed in the posterior horn. Electron microscopy showed a predominant nuclear immunostaining. A weaker but significant immunoreactive androgen receptor was also noted in the perinuclear/ intracysternal position. Moreover, no differences were found between male and female rats. Immuno-blotting demonstrated that the androgen receptor is expressed in both ventral and dorsal spinal cord, with an apparent molecular mass identical to that noted in other androgen-dependent tissues. The expression of androgen receptor in motoneurons corroborates the role of androgens in motoneuron growth, development and regeneration and underlies the possibility that androgen receptor abnormality leads to the motoneuron degeneration observed in X-linked spinal and bulbar muscular atrophy. Charles Sultan, Unité BEDR, Hôpital Lapeyronie, 34295 Montpellier, France

Neonatal handling alters learning in adult male and female rats in a task-specific manner

2007 ◽  
Vol 1154 ◽  
pp. 144-153 ◽  
Author(s):  
Therese A. Kosten ◽  
Hongjoo J. Lee ◽  
Jeansok J. Kim
Keyword(s):  
Adult Male ◽  
Female Rats ◽  
Male And Female ◽  
Neonatal Handling ◽  
Male And Female Rats ◽  
Specific Manner

scholarly journals Sex differences in ET-1 receptor expression and Ca2+ signaling in the IMCD

2013 ◽  
Vol 305 (8) ◽  
pp. F1099-F1104 ◽  
Author(s):  
Chunhua Jin ◽  
Joshua S. Speed ◽  
Kelly A. Hyndman ◽  
Paul M. O'Connor ◽  
David M. Pollock
Keyword(s):  
Sex Differences ◽  
Radioligand Binding ◽  
Collecting Duct ◽  
Receptor Activation ◽  
Receptor Expression ◽  
Female Rats ◽  
Male Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Intracellular Ca

The inner medullary collecting duct (IMCD) is the nephron segment with the highest production of endothelin-1 (ET-1) and the greatest expression of ET-1 receptors that function to adjust Na+ and water balance. We have reported that male rats have reduced natriuresis in response to direct intramedullary infusion of ET-1 compared with female rats. Our aim was to determine whether alterations of ET-1 receptor expression and downstream intracellular Ca2+ signaling within the IMCD could account for these sex differences. IMCDs from male and female rats were isolated for radioligand binding or microdissected for intracellular Ca2+ ([Ca2+]i) measurement by fluorescence imaging of fura-2 AM. IMCD from male and female rats had similar ETB expression (655 ± 201 vs. 567 ± 39 fmol/mg protein, respectively), whereas male rats had significantly higher ETA expression (436 ± 162 vs. 47 ± 29 fmol/mg protein, respectively; P < 0.05). The [Ca2+]i response to ET-1 was significantly greater in IMCDs from male compared with female rats (288 ± 52 vs. 118 ± 32 AUC, nM × 3 min, respectively; P < 0.05). In IMCDs from male rats, the [Ca2+]i response to ET-1 was significantly blunted by the ETA antagonist BQ-123 but not by the ETB antagonist BQ-788 (control: 137 ± 27; BQ-123: 53 ± 11; BQ-788: 84 ± 25 AUC, nM × 3 min; P < 0.05), consistent with greater ETA receptor function in male rats. These data demonstrate a sex difference in ETA receptor expression that results in differences in ET-1 Ca2+ signaling in IMCD. Since activation of ETA receptors is thought to oppose ETB receptor activation, enhanced ETA function in male rats could limit the natriuretic effects of ETB receptor activation.

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