Differences in developmental changes in GABAergic response between bushy and stellate cells in the rat anteroventral cochlear nucleus

2012 ◽  
Vol 30 (5) ◽  
pp. 397-403 ◽  
Author(s):  
Ning‐ying Song ◽  
Hai‐bo Shi ◽  
Chun‐yan Li ◽  
Shan‐kai Yin
Keyword(s):  
Cochlear Nucleus ◽  
Stellate Cells ◽  
Developmental Changes ◽  
Anteroventral Cochlear Nucleus

scholarly journals Development of Spontaneous Miniature EPSCs in Mouse AVCN Neurons During a Critical Period of Afferent-Dependent Neuron Survival

2007 ◽  
Vol 97 (1) ◽  
pp. 635-646 ◽  
Author(s):  
Yong Lu ◽  
Julie A. Harris ◽  
Edwin W. Rubel
Keyword(s):  
Critical Period ◽  
Stellate Cells ◽  
Synaptic Activity ◽  
Developmental Changes ◽  
Synaptic Inputs ◽  
Whole Cell Patch Clamp ◽  
Bushy Cell ◽  
Anteroventral Cochlear Nucleus ◽  
Decay Times ◽  
Bushy Cells

During a critical period prior to hearing onset, cochlea ablation leads to massive neuronal death in the mouse anteroventral cochlear nucleus (AVCN), where cell survival is believed to depend on glutamatergic input. We investigated the development of spontaneous miniature excitatory postsynaptic currents (mEPSCs) in AVCN neurons using whole cell patch-clamp techniques during [postnatal day 7 (P7)] and after (P14, P21) this critical period. We also examined the effects of unilateral cochlea ablation on mEPSC development. The two main AVCN neuron types, bushy and stellate cells, were distinguished electrophysiologically. Bushy cell mEPSCs became more frequent and faster between P7 and P14/P21 but with little change in amplitude. Dendritic filtering of mEPSCs was not detected as indicated by the lack of correlation between 10 and 90% rise times and decay time constants. Seven days after cochlea ablation at P7 or P14, mEPSCs in surviving bushy cells were similar to controls, except that rise and decay times were positively correlated ( R = 0.31 and 0.14 for surgery at P7 and P14, respectively). Consistent with this evidence for a shift of synaptic activity from the somata to the dendrites, SV2 staining (a synaptic vesicle marker) forms a ring around somata of control but not experimental bushy cells. In contrast, mEPSCs of stellate cells showed few significant changes over these ages with or without cochlea ablation. Taken together, mEPSCs in mouse AVCN bushy cells show dramatic developmental changes across this critical period, and cochlea ablation may lead to the emergence of excitatory synaptic inputs impinging on bushy cell dendrites.

Regularity analysis in a compartmental model of chopper units in the anteroventral cochlear nucleus

1991 ◽  
Vol 65 (3) ◽  
pp. 606-629 ◽  
Author(s):  
M. I. Banks ◽  
M. B. Sachs
Keyword(s):  
Cochlear Nucleus ◽  
Auditory Nerve ◽  
Compartmental Model ◽  
Dendritic Tree ◽  
Stellate Cells ◽  
Physiological Data ◽  
Response Patterns ◽  
Anteroventral Cochlear Nucleus

1. We investigate the discharge patterns of chopper units in the anteroventral cochlear nucleus (AVCN) by developing an equivalent cylinder compartmental model of AVCN stellate cells, which are the sources of the chopper response pattern. The model consists of a passive dendritic tree connected to somatic and axonal compartments with voltage-sensitive channels. Synaptic inputs to the model are simulated auditory nerve fiber responses to best-frequency tones. 2. We adjust the anatomic and electrical parameters of the model to agree with available intracellular data from stellate cells in the AVCN of the mouse and the cat and compare the response of the model to injected current with responses recorded in vitro. The model shows approximately linear current-voltage characteristics for small hyperpolarizing currents. The model's input resistance and the time course of its response to hyperpolarizing current applied at the soma are comparable with those measured from stellate cells in vitro. In response to sustained depolarizing current, the model fires repetitively with nearly perfect regularity, a property also observed in vitro. 3. Auditory nerve inputs to the cell are modeled as deadtime-modified Poisson processes with a multiexponential adaptation in the Poisson rate. We are able to adjust the number, rate, and location of excitatory and inhibitory inputs to the model and succeed in simulating chopper response patterns seen in vivo. 4. Chopper units exhibit a variety of regularity and adaptation patterns in response to tone stimuli. Physiological data from brain slice experiments and experiments in vivo imply that this heterogeneity is primarily due to differences in input configurations. By systematically varying the number and position of excitatory and inhibitory inputs, we can simulate a range of chopper response patterns. 5. We quantify the regularity of the model's response using the coefficient of variation (CV) of the interspike interval. We find that the CV decreases, i.e., the regularity increases, as the number of converging inputs or their distance from the soma increases. The regularity of the output is more sensitive to the number of converging inputs than to their location on the dendritic tree. The statistics of the first spike latency (FSL) are also sensitive to the configuration of excitatory inputs. The mean and minimum FSL are more sensitive to the electrotonic distance of the inputs from the soma than to the number of inputs, whereas the standard deviation of the FSL is highly dependent on the number of converging inputs and is nearly independent of their location.(ABSTRACT TRUNCATED AT 400 WORDS)

Commissural glycinergic inhibition of bushy and stellate cells in the anteroventral cochlear nucleus

Neuroreport ◽  
2002 ◽  
Vol 13 (4) ◽  
pp. 555-558 ◽  
Author(s):  
Alexandre L. Babalian ◽  
Anne-Valerie Jacomme ◽  
John R. Doucet ◽  
David K. Ryugo ◽  
Eric M. Rouiller
Keyword(s):  
Cochlear Nucleus ◽  
Stellate Cells ◽  
Anteroventral Cochlear Nucleus ◽  
Glycinergic Inhibition

Regularity and latency of units in ventral cochlear nucleus: implications for unit classification and generation of response properties

1988 ◽  
Vol 60 (1) ◽  
pp. 1-29 ◽  
Author(s):  
E. D. Young ◽  
J. M. Robert ◽  
W. P. Shofner
Keyword(s):  
Standard Deviation ◽  
Cochlear Nucleus ◽  
Interspike Interval ◽  
Rate Adaptation ◽  
Stellate Cells ◽  
Instantaneous Rate ◽  
Ventral Cochlear Nucleus ◽  
Bushy Cell ◽  
Low Pass ◽  
The Mean

1. The responses of neurons in the ventral cochlear nucleus (VCN) of decerebrate cats are described with regard to their regularity of discharge and latency. Regularity is measured by estimating the mean and standard deviation of interspike intervals as a function of time during responses to short tone bursts (25 ms). This method extends the usual interspike-interval analysis based on interval histograms by allowing the study of temporal changes in regularity during transient responses. The coefficient of variation (CV), equal to the ratio of standard deviation to mean interspike interval, is used as a measure of irregularity. Latency is measured as the mean and standard deviation of the latency of the first spike in response to short tone bursts, with 1.6-ms rise times. 2. The regularity and latency properties of the usual PST histogram response types are shown. Five major PST response type classes are used: chopper, primary-like, onset, onset-C, and unusual. The presence of a prepotential in a unit's action potentials is also noted; a prepotential implies that the unit is recorded from a bushy cell. 3. Units with chopper PST histograms give the most regular discharge. Three varieties of choppers are found. Chop-S units (regular choppers) have CVs less than 0.35 that are approximately constant during the response; chop-S units show no adaptation of instantaneous rate, as measured by the inverse of the mean interspike interval. Chop-T units have CVs greater than 0.35, show an increase in irregularity during the response and show substantial rate adaptation. Chop-U units have CVs greater than 0.35, show a decrease in irregularity during the response, and show a variety of rate adaptation behaviors, including negative adaptation (an increase in rate during a short-tone response). Irregular choppers (chop-T and chop-U units) rarely have CVs greater than 0.5. Choppers have the longest latencies of VCN units; all three groups have mean latencies at least 1 ms longer than the shortest auditory nerve (AN) fiber mean latencies. 4. Chopper units are recorded from stellate cells in VCN (35, 42). Our results for chopper units suggest a model for stellate cells in which a regularly firing action potential generator is driven by the summation of the AN inputs to the cell, where the summation is low-pass filtered by the membrane capacitance of the cell.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Multidimensional Characterization and Differentiation of Neurons in the Anteroventral Cochlear Nucleus

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e29965 ◽  
Author(s):  
Marei Typlt ◽  
Bernhard Englitz ◽  
Mandy Sonntag ◽  
Susanne Dehmel ◽  
Cornelia Kopp-Scheinpflug ◽  
...  
Keyword(s):  
Cochlear Nucleus ◽  
Anteroventral Cochlear Nucleus

Wiener kernel analysis of responses from anteroventral cochlear nucleus neurons

1984 ◽  
Vol 14 (2) ◽  
pp. 155-174 ◽  
Author(s):  
Robert E. Wickesberg ◽  
John W. Dickson ◽  
Mary Morton Gibson ◽  
C. Daniel Geisler
Keyword(s):  
Cochlear Nucleus ◽  
Kernel Analysis ◽  
Wiener Kernel ◽  
Anteroventral Cochlear Nucleus
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