Induction of neural differentiation by the transcription factor NeuroD2

2011 ◽  
Vol 30 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Kirsten Messmer ◽  
Wei‐Bin Shen ◽  
Mary Remington ◽  
Paul S. Fishman
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation

Forkhead family transcription factor FoxO and neural differentiation

Neurogenetics ◽  
2012 ◽  
Vol 13 (2) ◽  
pp. 105-113 ◽  
Author(s):  
Qiang Wen ◽  
Haitao Wang ◽  
Peter J. Little ◽  
Remi Quirion ◽  
Wenhua Zheng
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation

scholarly journals Repressor Element 1 Silencing Transcription Factor Couples Loss of Pluripotency with Neural Induction and Neural Differentiation

Stem Cells ◽  
2012 ◽  
Vol 30 (3) ◽  
pp. 425-434 ◽  
Author(s):  
Chiara Soldati ◽  
Angela Bithell ◽  
Caroline Johnston ◽  
Kee-Yew Wong ◽  
Siaw-Wei Teng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation ◽  
Neural Induction ◽  
Repressor Element

scholarly journals miR-124, -128, and -137 Orchestrate Neural Differentiation by Acting on Overlapping Gene Sets Containing a Highly Connected Transcription Factor Network

Stem Cells ◽  
2015 ◽  
Vol 34 (1) ◽  
pp. 220-232 ◽  
Author(s):  
Márcia C. T. Santos ◽  
Allison N. Tegge ◽  
Bruna R. Correa ◽  
Swetha Mahesula ◽  
Luana Q. Kohnke ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation ◽  
Transcription Factor Network ◽  
Overlapping Gene ◽  
Gene Sets

scholarly journals The transcription factor E2A drives neural differentiation in pluripotent cells

Development ◽  
2020 ◽  
Vol 147 (12) ◽  
pp. dev184093
Author(s):  
Chandrika Rao ◽  
Mattias Malaguti ◽  
John O. Mason ◽  
Sally Lowell
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation ◽  
Pluripotent Cells

scholarly journals The transcription factor E2A drives neural differentiation in pluripotent cells

2019 ◽  
Author(s):  
Chandrika Rao ◽  
Mattias Malaguti ◽  
John O. Mason ◽  
Sally Lowell
Keyword(s):  
Transcription Factor ◽  
Neural Differentiation ◽  
Bhlh Transcription Factor ◽  
Pluripotent Cells ◽  
Neural Lineage ◽  
Binding Partner ◽  
Helix Loop Helix ◽  
Id Proteins ◽  
Mouse Cells ◽  
Extracellular Signalling

AbstractThe intrinsic mechanisms that link extracellular signalling to the onset of neural differentiation are not well understood. In pluripotent mouse cells, BMP blocks entry into the neural lineage via transcriptional upregulation of Inhibitor of Differentiation (Id) factors. We have previously identified that the major binding partner of Id proteins in pluripotent cells is the basic helix-loop-helix (bHLH) transcription factor (TF), E2A. Id1 can prevent E2A from forming heterodimers with bHLH TFs or from forming homodimers. Here, we show that overexpression of a forced E2A homodimer is sufficient to drive robust neural commitment in pluripotent cells, even under non-permissive conditions. Conversely, we find that E2A null cells display a defect in their neural differentiation capacity. E2A acts as an upstream activator of neural lineage genes, including Sox1 and Foxd4, and as a repressor of Nodal signalling. Our results suggest a crucial role for E2A in establishing neural lineage commitment in pluripotent cells.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

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