Mechanism characterization of bacterial inactivation of atmospheric air plasma gas and activated water using bioluminescence technology

2019 ◽  
Vol 53 ◽  
pp. 18-25 ◽  
Author(s):  
Shyam Suwal ◽  
Claudia P. Coronel-Aguilera ◽  
Jameson Auer ◽  
Bruce Applegate ◽  
Allen L. Garner ◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Apurva D. Patange ◽  
Jeremy C. Simpson ◽  
James F. Curtin ◽  
Catherine M. Burgess ◽  
P. J. Cullen ◽  
...  

AbstractBiofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies were efficient in controlling, or completely inactivating, the target bacterial biofilms. Viability and metabolic assays, along with microscopy analysis, revealed that atmospheric air plasma and airborne acoustic ultrasound damaged both the bacterial biofilm cells and its structural integrity. Scanning electron microscopy images highlighted the disruption of the biofilms and pore formation in bacterial cells exposed to both the plasma and acoustic treatments. Elevated reactive oxygen and nitrogen species in bacterial cells treated with atmospheric air plasma, demonstrated their primary role in the observed bacterial inactivation process. Our findings provide potential antimicrobial strategies to combat bacterial biofilms in the food and healthcare sectors.


2016 ◽  
Vol 41 (1) ◽  
pp. e12889 ◽  
Author(s):  
S.K. Pankaj ◽  
C. Bueno-Ferrer ◽  
L. O'Neill ◽  
B.K. Tiwari ◽  
Paula Bourke ◽  
...  

Catalysts ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 462 ◽  
Author(s):  
Po-Chia Huang ◽  
Sanjaya Brahma ◽  
Po-Yen Liu ◽  
Jow-Lay Huang ◽  
Sheng-Chang Wang ◽  
...  

Here, we demonstrate the enhanced water-splitting performance (I = 10 mA/cm2, Tafel slope = 60 mV/dec, onset potential = −80 mV) of atmospheric air plasma treated (AAPT) SnS thin films by the hydrogen evolution reaction (HER). The as prepared SnS films were subjected to Atmospheric Air Plasma Treatment (AAPT) which leads to formation of additional phases of Sn and SnO2 at plasma powers of 150 W and 250 W, respectively. The AAPT treatment at 150 W leads to the evaporation of the S atoms as SO2 generates a number of S-vacancies and Sn active edge sites over the surface of the SnS thin film. S-vacancies also create Sn active edge sites, surface p-type pinning that tunes the suitable band positions, and a hydrophilic surface which is beneficial for hydrogen adsorption/desorption. At high plasma power (250 W), the surface of the SnS films becomes oxidized and degrades the HER performance. These results demonstrate that AAPT (150 W) is capable of improving the HER performance of SnS thin films and our results indicate that SnS thin films can work as efficient electrocatalysts for HER.


2020 ◽  
Vol 2 (2) ◽  
pp. 48-49

Ivory nuts, produced by palms from the genus Phytelephas, possess a hard and microporous endosperm with a strong resemblance to elephant ivory. The nuts sustainable appeal made them popular as eco-friendly substitutes to ivory since they promote the development of forest communities without contributing to deforestation and animal poaching. In addition, they have been commercialized as microbeads to replace microplastics in cosmetic applications. However, this material is vulnerable to deterioration by micro-organisms and insects, as they are predominantly constituted by β-1,4-mannan, a hydrophilic polysaccharide similar to cellulose. In this context, seed endosperm was treated for 80 s by an atmospheric air plasma jet so as to modify its wettability, as plasma has been widely studied recently for seed disinfection and surface modification. Plasma treated samples were characterized by the water contact angle, AFM, and Raman imaging. Water contact angle results showed an increase from (31.5 ± 8.7)º to (78.9 ± 5.4)º, indicating incorporation of hydrophobic moieties to the sample surface. In turn, AFM images demonstrate the formation of a rough and heterogeneous coating that resembles epicuticular wax layers. Furthermore, principal component analysis of Raman imaging results evidenced contributions from wax (1156, 1170 and 1410 cm-1), carbohydrates (1020, 1080 and 1106 cm-1), and lignin (1573, 1635 and 1662 cm-1). These results indicate that plasma treatment promoted the migration of hydrophobic waxes to the surface and their crosslinking with fragmented cell wall material such as mannan, xylan, and lignin, promoting seed hydrophobization with no need for additional precursors or generation of side products.


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