Influence of growth and treatment temperature on Staphylococcus aureus resistance to pulsed electric fields: Relationship with membrane fluidity

2016 ◽  
Vol 37 ◽  
pp. 161-169 ◽  
Author(s):  
G. Cebrián ◽  
S. Condón ◽  
P. Mañas
Keyword(s):  
Staphylococcus Aureus ◽  
Membrane Fluidity ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Treatment Temperature

scholarly journals Component release after exposure of Staphylococcus aureus cells to pulsed electric fields

2021 ◽  
pp. 102838
Author(s):  
Víctor Freire ◽  
Giuseppe Lattanzio ◽  
Irene Orera ◽  
Pilar Mañas ◽  
Guillermo Cebrián
Keyword(s):  
Staphylococcus Aureus ◽  
Electric Fields ◽  
Pulsed Electric Fields

scholarly journals Inactivation of Mycobacterium paratuberculosis by Pulsed Electric Fields

2001 ◽  
Vol 67 (6) ◽  
pp. 2833-2836 ◽  
Author(s):  
Neil J. Rowan ◽  
Scott J. MacGregor ◽  
John G. Anderson ◽  
Douglas Cameron ◽  
Owen Farish
Keyword(s):  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Cellular Level ◽  
Treatment Temperature ◽  
Cow’S Milk ◽  
Mycobacterium Paratuberculosis ◽  
Cow's Milk ◽  
High Temperature Short Time ◽  
Peptone Water ◽  
Short Time

ABSTRACT The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosiscells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosisat lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions ofM. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.

Influence of treatment temperature on the inactivation of Listeria innocua by pulsed electric fields

LWT ◽  
2005 ◽  
Vol 38 (2) ◽  
pp. 167-172 ◽  
Author(s):  
D.R. Sepulveda ◽  
M.M. Góngora-Nieto ◽  
M.F. San-Martin ◽  
G.V. Barbosa-Cánovas
Keyword(s):  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Treatment Temperature ◽  
Listeria Innocua

scholarly journals Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1799 ◽  
Author(s):  
Vitalij Novickij ◽  
Jurgita Švedienė ◽  
Algimantas Paškevičius ◽  
Svetlana Markovskaja ◽  
Eglė Lastauskienė ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
Electric Field ◽  
Antibiotic Treatment ◽  
Electric Fields ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Combined Treatment ◽  
Pulsed Electric Fields ◽  
Methicillin Resistant ◽  
Physical Methods

Treatment of bacteria-associated infections is complicated and antibiotic treatment alone is often inadequate to overcome biofilm infections. Physical methods allow overcoming this problem and propose solutions that are non-dependent on drug resistance. In this work, we investigated the feasibility of pulsed electric fields for sensitization of MRSA to common antibiotics. We analyzed the efficacy of inactivation of methicillin-resistant Staphylococcus aureus in 5–20 kV/cm electric field separately and in combination with gentamicin, doxycycline, ciprofloxacin, sulfamethoxazole, and vancomycin. Combined treatment allowed using up to 1000-fold smaller concentrations of antibiotics to induce the same inactivation of S. aureus.

Inactivation of Pseudomonas fluorescens in Skim Milk by Combinations of Pulsed Electric Fields and Organic Acids

2005 ◽  
Vol 68 (6) ◽  
pp. 1232-1235 ◽  
Author(s):  
JUAN J. FERNÁNDEZ-MOLINA ◽  
BILGE ALTUNAKAR ◽  
DANIELA BERMÚDEZ-AGUIRRE ◽  
BARRY G. SWANSON ◽  
GUSTAVO V. BARBOSA-CÁNOVAS
Keyword(s):  
Acetic Acid ◽  
Organic Acids ◽  
Pseudomonas Fluorescens ◽  
Propionic Acid ◽  
Electric Fields ◽  
Skim Milk ◽  
Pulsed Electric Fields ◽  
Treatment Temperature ◽  
Initial Concentration ◽  
Ph Range

Pseudomonas fluorescens suspended in skim milk was inactivated by application of pulsed electric fields (PEF) either alone or in combination with acetic or propionic acid. The initial concentration of microorganisms ranged from 105 to 106 CFU/ml. Addition of acetic acid and propionic acid to skim milk inactivated 0.24 and 0.48 log CFU/ml P. fluorescens, respectively. Sets of 10, 20, and 30 pulses were applied to the skim milk using exponentially decaying pulses with pulse lengths of 2 μs and pulse frequencies of 3 Hz. Treatment temperature was maintained between 16 and 20°C. In the absence of organic acids, PEF treatment of skim milk at field intensities of 31 and 38 kV/cm reduced P. fluorescens populations by 1.0 to 1.8 and by 1.2 to 1.9 log CFU/ml, respectively. Additions of acetic and propionic acid to the skim milk in a pH range of 5.0 to 5.3 and PEF treatment at 31, 33, and 34 kV/cm, and 36, 37, and 38 kV/cm reduced the population of P. fluorescens by 1.4 and 1.8 log CFU/ml, respectively. No synergistic effect resulted from the combination of PEF with acetic or propionic acid.

scholarly journals Inactivation of Escherichia coli and Staphylococcus aureus by Pulsed Electric Fields Increases with Higher Bacterial Population and with Agitation of Liquid Medium

2014 ◽  
Vol 77 (7) ◽  
pp. 1219-1223
Author(s):  
SILVIA BONETTA ◽  
SARA BONETTA ◽  
MONICA BELLERO ◽  
MARCO PIZZICHEMI ◽  
ELISABETTA CARRARO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Electric Fields ◽  
Bacterial Population ◽  
Electric Pulse ◽  
Pulsed Electric Fields ◽  
Microbial Inactivation ◽  
Bacterial Inactivation ◽  
E Coli ◽  
And Staphylococcus Aureus

Inactivation of Escherichia coli, E. coli O157:H7, and Staphylococcus aureus in liquid media by pulsed electric fields (PEF) was conducted at varying bacterial populations with and without sample agitation. A laboratory-scale PEF batch unit with a rectangular electric pulse was used, operating under the following conditions: 25 kV/cm (E. coli, E. coli O157:H7) and 30 kV/cm (S. aureus) electric field strengths, 1-μs pulse width, 1-Hz pulse repetition rate, and 20 to 350 pulses for all samples. Not surprisingly, bacterial inactivation (for all three strains) increased with increasing pulse number, achieving the highest reduction at 350 pulses. Log CFU per milliliter microbial inactivation increased commensurately with increasing bacterial population (P < 0.05) but only when samples were treated with more than 200 pulses. For example, when E. coli was treated with 200 pulses at 105 CFU/ml, inactivation was only 3.0 Log versus 4.8 Log at the 1010 inoculation level. When E. coli O157:H7 was treated with 200 pulses at 105 CFU/ml, inactivation was only 2.5 Log versus 4.6 Log at the 1010 inoculation level. When S. aureus was treated with 200 pulses at 106 CFU/ml, inactivation was only 2.6 Log versus 4.8 Log at the 1010 inoculation level. Inactivation of populations was also found to be statistically greater (P < 0.05) when liquid samples were agitated, in comparison to nonagitated samples. Because PEF inactivation activity is influenced by bacterial population and sample agitation, future studies should carefully consider these factors in experimental designs and/or scaled-up industry application.

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