Effects of high hydrostatic pressure on Eimeria acervulina pathogenicity, immunogenicity and structural integrity

2007 ◽  
Vol 8 (2) ◽  
pp. 259-268 ◽  
Author(s):  
Adrienne E.H. Shearer ◽  
Gary C. Wilkins ◽  
Mark C. Jenkins ◽  
Kalmia E. Kniel
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Structural Integrity ◽  
Eimeria Acervulina

High Hydrostatic Pressure and UV Light Treatment of Produce Contaminated with Eimeria acervulina as a Cyclospora cayetanensis Surrogate

2007 ◽  
Vol 70 (12) ◽  
pp. 2837-2842 ◽  
Author(s):  
KALMIA E. KNIEL ◽  
ADRIENNE E. H. SHEARER ◽  
JENNIFER L. CASCARINO ◽  
GARY C. WILKINS ◽  
MARK C. JENKINS
Keyword(s):  
Hydrostatic Pressure ◽  
Uv Radiation ◽  
High Hydrostatic Pressure ◽  
Broiler Chickens ◽  
Uv Light ◽  
The United States ◽  
Inoculum Level ◽  
Cyclospora Cayetanensis ◽  
Eimeria Acervulina ◽  
High Hydrostatic Pressure Processing

The prevalence, size, genome, and life cycle of Eimeria acervulina make this organism a good surrogate for Cyclospora cayetanensis, a protozoan that causes gastroenteritis in humans, including recent outbreaks in the United States and Canada associated with contaminated raspberries and basil. Laboratory studies of C. cayetanensis are difficult because of the lack of readily available oocysts and of infection models and assays. UV radiation and high-hydrostatic-pressure processing (HPP) are both safe technologies with potential for use on fresh produce. Raspberries and basil were inoculated with sporulated E. acervulina oocysts at high (106 oocysts) and low (104 oocysts) levels, and inoculated and control produce were treated with UV (up to 261 mW/cm2) or HPP (550 MPa at 40°C for 2 min). Oocysts recovered from produce were fed to 3-week-old broiler chickens, which were scored for weight gain, oocyst shedding, and lesions at 6 days postinoculation. Oocysts exhibited enhanced excystation on raspberries but not on basil. Birds fed oocysts from UV-treated raspberries had reduced infection rates, which varied with oocyst inoculum level and UV intensity. Birds fed oocysts from UV-treated raspberries (104 oocysts) were asymptomatic but shed oocysts, and birds fed oocysts from UV-treated basil (104 oocysts) were asymptomatic and did not shed oocysts. Birds fed oocysts from HPP-treated raspberries and basil were asymptomatic and did not shed oocysts. These results suggest that UV radiation and HPP may be used to reduce the risk for cyclosporiasis infection associated with produce. Both treatments yielded healthy animals; however, HPP was more effective, as indicated by results for produce with higher contamination levels.

scholarly journals Pressure-induced oversaturation and phase transition in zeolitic imidazolate frameworks with remarkable mechanical stability

2015 ◽  
Vol 44 (10) ◽  
pp. 4498-4503 ◽  
Author(s):  
Pu Zhao ◽  
Thomas D. Bennett ◽  
Nicola P. M. Casati ◽  
Giulio I. Lampronti ◽  
Stephen A. Moggach ◽  
...  
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Structural Integrity ◽  
Mechanical Stability ◽  
Zeolitic Imidazolate Frameworks ◽  
Metal Substitution

ZIF-7/9 are shown be able to withstand high hydrostatic pressure unaffected by metal substitution, whilst retaining porosity and structural integrity.

Survival of mouse blastocysts after low-temperature preservation under high pressure

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
Low Temperature ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Freezing Point ◽  
Significant Loss ◽  
Embryo Cryopreservation ◽  
Subzero Temperatures ◽  
Survival Capacity

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

Effect of High Hydrostatic Pressure on Endothelial Lipase Expression*

2010 ◽  
Vol 37 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Can-Xin XU ◽  
Chun WANG ◽  
Bing-Yang ZHU ◽  
Zhi-Ping GAO ◽  
Di-Xian LUO ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Endothelial Lipase ◽  
Lipase Expression
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