Post swim-up versus original sperm quality, and strict criteria morphology, it’s influence on fertilization rate in in vitro fertilization program: a pilot study

2004 ◽  
Vol 1271 ◽  
pp. 181-184 ◽  
Author(s):  
M. Michaeli ◽  
S. Peer ◽  
S. Anderman ◽  
S. Ballas ◽  
A. Ellenbogen
Keyword(s):  
Pilot Study ◽  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Fertilization Program ◽  
Vitro Fertilization

The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program

1996 ◽  
Vol 66 (3) ◽  
pp. 430-434 ◽  
Author(s):  
Eli Geva ◽  
Benjamin Bartoov ◽  
Natalia Zabludovsky ◽  
Joseph B. Lessing ◽  
Liat Lerner-Geva ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Fertilization Rate ◽  
Human Spermatozoa ◽  
Antioxidant Treatment ◽  
Fertilization Program ◽  
Vitro Fertilization

Improved fertilization rate in an in vitro fertilization program by egg yolk-treated sperm

1992 ◽  
Vol 58 (1) ◽  
pp. 197-198 ◽  
Author(s):  
Yona Barak ◽  
Ami Amit ◽  
Joseph B. Lessing ◽  
Gedalia Paz ◽  
Zwi T. Homonnai ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Egg Yolk ◽  
Fertilization Rate ◽  
Fertilization Program ◽  
Vitro Fertilization

scholarly journals Standardization of sperm management for laboratory assessment of sperm quality and in vitro fertilization in Eurasian perch (Perca fluviatilis)

2021 ◽  
Author(s):  
Z. Bokor ◽  
D. Żarski ◽  
K. Palińska-Żarska ◽  
S. Krejszeff ◽  
J. Król ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Eurasian Perch ◽  
Vitro Fertilization ◽  
Progressive Motility ◽  
Egg Ratio ◽  
Significant Difference ◽  
Activating Solution

AbstractSperm handling procedures and its usage for in vitro fertilization are crucial for standardized experimental operations on evaluation of reproductive performance, gamete quality, and optimization of fertilization protocols. In our study, the changes in perch sperm motility parameters within 6-h chilled storage and using 4 different activating solutions were compared. Eight different sperm-to-egg ratio was also compared during fertilization. Sperm activated with modified activating solution for cyprinids (78±11%), common perch activating solution (68±16%), modified Lahnsteiner activating solution (75±16%), and Woynárovich solution (76±13%) showed similar progressive motility at 10 s after activation. At 30 s after activation, progressive motility decreased below 5%, regardless the activating solution used. Progressive motility decreased significantly already after 2 h of storage (51±19%) in comparison with 0 h (78±5%). The highest average fertilization rate (using common perch activating solution) was observed with a sperm-to-egg ratio 2.5×105:1 (80±9%), where the smallest variability in the values was also recorded (coefficient of variation: 11%). However, no significant difference was detected among the 8 sperm-to-egg ratio groups. According to our findings, undiluted fresh perch sperm is recommended to use in 1 h post-stripping. Modified Lahnsteiner’s activating solution can be applied efficiently for quality assessment where common perch activating solution is applicable for fertilization in Eurasian perch. A sperm-to-egg ratio 2.5×105:1 already allows to achieve a high fertilization rate; however, the finding is needed to be tested also at hatchery level (higher number of eggs).

scholarly journals A Brief Incubation of Cumulus-Enclosed Mouse Eggs in a Calcium-Free Medium Containing a High Concentration of Calcium-Chelator Markedly Improves Preimplantation Development

2020 ◽  
Vol 17 (10) ◽  
pp. 3505
Author(s):  
Valeria Merico ◽  
Silvia Garagna ◽  
Maurizio Zuccotti
Keyword(s):  
In Vitro Fertilization ◽  
Mammalian Species ◽  
Developmental Rate ◽  
Cumulus Cells ◽  
Fertilization Rate ◽  
Preimplantation Development ◽  
High Concentration ◽  
Vitro Fertilization ◽  
Positive Effects

The presence of cumulus cells (CCs) surrounding ovulated eggs is beneficial to in vitro fertilization and preimplantation development outcomes in several mammalian species. In the mouse, this contribution has a negligible effect on the fertilization rate; however, it is not yet clear whether it has positive effects on preimplantation development. Here, we compared the rates of in vitro fertilization and preimplantation development of ovulated B6C3F1 CC-enclosed vs. CC-free eggs, the latter obtained either after a 5 min treatment in M2 medium containing hyaluronidase or after 5–25 min in M2 medium supplemented with 34.2 mM EDTA (M2-EDTA). We found that, although the maintenance of CCs around ovulated eggs does not increment their developmental rate to blastocyst, the quality of the latter is significantly enhanced. Most importantly, for the first time, we describe a further quantitative and qualitative improvement, on preimplantation development, when CC-enclosed eggs are isolated from the oviducts in M2-EDTA and left in this medium for a total of 5 min prior to sperm insemination. Altogether, our results establish an important advancement in mouse IVF procedures that would be now interesting to test on other mammalian species.

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