A novel RNA helicase-like protein during early embryonic development in silkworm Bombyx mori: Molecular characterization and intracellular localization

2006 ◽  
Vol 36 (12) ◽  
pp. 911-920 ◽  
Author(s):  
H. Sawada ◽  
Y. Yamahama ◽  
T. Yamamoto ◽  
K. Mase ◽  
H. Ogawa ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bombyx Mori ◽  
Molecular Characterization ◽  
Intracellular Localization ◽  
Rna Helicase ◽  
Early Embryonic Development ◽  
Silkworm Bombyx Mori

Gene Expression Profiling in the Silkworm, Bombyx mori, During Early Embryonic Development

2006 ◽  
Vol 23 (6) ◽  
pp. 517-528 ◽  
Author(s):  
Sun-Mee Hong ◽  
Si-Kab Nho ◽  
Nam-Soon Kim ◽  
Jin-Sung Lee ◽  
Seok-Woo Kang
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Gene Expression Profiling ◽  
Bombyx Mori ◽  
Expression Profiling ◽  
Early Embryonic Development ◽  
Silkworm Bombyx Mori

scholarly journals DNA Methylation Is Correlated with Gene Expression during Diapause Termination of Early Embryonic Development in the Silkworm (Bombyx mori)

2020 ◽  
Vol 21 (2) ◽  
pp. 671 ◽  
Author(s):  
Bing Li ◽  
Pei Hu ◽  
Lin-Bao Zhu ◽  
Ling-Ling You ◽  
Hui-Hua Cao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Embryonic Development ◽  
Bombyx Mori ◽  
Transcription Initiation ◽  
Early Embryonic Development ◽  
Dna Modification ◽  
Promoter Regions ◽  
Short Interspersed Elements ◽  
Silkworm Bombyx Mori

DNA modification is a naturally occurring DNA modification in prokaryotic and eukaryotic organisms and is involved in several biological processes. Although genome-wide methylation has been studied in many insects, the understanding of global and genomic DNA methylation during insect early embryonic development, is lacking especially for insect diapause. In this study, we analyzed the relationship between DNA methylomes and transcriptomes in diapause-destined eggs compared to diapause-terminated eggs in the silkworm, Bombyx mori (B. mori). The results revealed that methylation was sparse in this species, as previously reported. Moreover, methylation levels in diapause-terminated eggs (HCl-treated) were 0.05% higher than in non-treated eggs, mainly due to the contribution of CG methylation sites. Methylation tends to occur in the coding sequences and promoter regions, especially at transcription initiation sites and short interspersed elements. Additionally, 364 methylome- and transcriptome-associated genes were identified, which showed significant differences in methylation and expression levels in diapause-destined eggs when compared with diapause-terminated eggs, and 74% of methylome and transcriptome associated genes showed both hypermethylation and elevated expression. Most importantly, Kyoto Encyclopaedia of Genes and Genomes (KEGG) analyses showed that methylation may be positively associated with Bombyx mori embryonic development, by regulating cell differentiation, metabolism, apoptosis pathways and phosphorylation. Through analyzing the G2/M phase-specific E3 ubiquitin-protein ligase (G2E3), we speculate that methylation may affect embryo diapause by regulating the cell cycle in Bombyx mori. These findings will help unravel potential linkages between DNA methylation and gene expression during early insect embryonic development and insect diapause.

scholarly journals Vitellogenin receptor selectively endocytoses female-specific and highly-expressed hemolymph proteins in the silkworm, Bombyx mori

2017 ◽  
Vol 95 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Chaoshan Han ◽  
Enxiang Chen ◽  
Guanwang Shen ◽  
Zhixin Peng ◽  
Yinying Xu ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bombyx Mori ◽  
Sf9 Cells ◽  
Tissue Localization ◽  
Vitellogenin Receptor ◽  
Hemolymph Proteins ◽  
Female Specific ◽  
Oocyte Membrane ◽  
Silkworm Bombyx Mori

VgR, a member of the LDLR family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, BmVgR has been shown to interact with BmSP1 in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with BmVg and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells. Parallel immunofluorescence showed that most BmVg and BmVgR were localized in the inner oocyte membrane, showing tissue localization similar to that of BmVg labeled with pHrodo Red absorbed by the ovaries on day 2 of pupation. Although BmSP1 showed localization similar to BmVgR during the same phase, little BmSP1 was present in the ovary. Additionally, BmSP1 did not exist in ovaries when the ovaries contained BmVgR on day 5 of pupation, suggesting that BmSP1 in the ovaries was not endocytosed by BmVgR. In summary, BmVgR could facilitate uptake of BmVg by developing oocytes, but did not modulate in the transport of BmSP1.

Twenty-hydroxyecdysone produced by dephosphorylation and ecdysteroidogenesis regulates early embryonic development in the silkmoth, Bombyx mori

2020 ◽  
Vol 127 ◽  
pp. 103491
Author(s):  
Daiki Fujinaga ◽  
Junjie Gu ◽  
Hajime Kawahara ◽  
Mari H. Ogihara ◽  
Ikumi Kojima ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bombyx Mori ◽  
Early Embryonic Development

DNA methyltransferaseBmDnmt1andBmDnmt2in silkworm (Bombyx mori) and the regulation of silkworm embryonic development

2018 ◽  
Vol 100 (3) ◽  
pp. e21529 ◽  
Author(s):  
Bing Li ◽  
Pei Hu ◽  
Shang‐Zhi Zhang ◽  
Shahzad Toufeeq ◽  
Jie Wang ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bombyx Mori ◽  
Silkworm Bombyx Mori
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