Identification of low molecular weight aromatic compounds by gas chromatography–mass spectrometry (GC–MS) from kraft lignin degradation by three Bacillus sp.

2007 ◽  
Vol 59 (4) ◽  
pp. 292-296 ◽  
Author(s):  
Abhay Raj ◽  
M.M. Krishna Reddy ◽  
Ram Chandra
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Aromatic Compounds ◽  
Lignin Degradation ◽  
Kraft Lignin ◽  
Gas Chromatography Mass Spectrometry ◽  
Low Molecular Weight ◽  
Bacillus Sp ◽  
Chromatography Mass Spectrometry

scholarly journals Enzymic synthesis of indol-3-ylacetyl-myo-inositol galactoside

1982 ◽  
Vol 207 (2) ◽  
pp. 283-290 ◽  
Author(s):  
L J Corcuera ◽  
L Michalczuk ◽  
R S Bandurski
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Zea Mays ◽  
Catalytic Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Low Molecular Weight ◽  
Enzymic Synthesis ◽  
Chromatography Mass Spectrometry ◽  
Ylacetic Acid

Extracts of immature kernels of Zea mays catalysed the synthesis of indol-3-ylacetyl-myo-inositol galactoside from indol-3-ylacetyl-myo-inositol and UDP-galactose. Addition of 2-mercaptoethanol was required for stability of the catalytic activity during dialysis. The enzyme could be fractionated with (NH4)2SO4, and 55% of the activity was recovered in the 30-60%-saturation fraction. The product of the reaction contained radioactivity from UDP-[U-14C]galactose and was identified as indol-3-ylacetyl-myo-inositol galactoside by gas chromatography-mass spectrometry. Therefore a UDP-galactose:indol-3-ylacetyl-myo-inositol galactosyltransferase (indol-3-ylacetyl-myo-inositol galactoside synthase) is present in developing kernels of Zea mays. The description of this enzyme, together with the enzymes described in the accompanying paper [Michalczuk & Bandurski (1982) Biochem. J. 207, 273-281] for the synthesis of indol-3-ylacetylglucose and indol-3-ylacetyl-myo-inositol, now provides mechanisms for the biosynthesis of one-half of the low-molecular-weight esters of indol-3-ylacetic acid in Zea mays.

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