Adhesion to metal sulfide surfaces by cells of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans

2006 ◽  
Vol 83 (1-4) ◽  
pp. 245-254 ◽  
Author(s):  
K. Harneit ◽  
A. Göksel ◽  
D. Kock ◽  
J.-H. Klock ◽  
T. Gehrke ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Metal Sulfide ◽  
Acidithiobacillus Thiooxidans ◽  
Leptospirillum Ferrooxidans

scholarly journals Adaptation of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans strains on sphalerite concentrate from mining waste

Respuestas ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 72-83
Author(s):  
Lina M. Miranda Arroyave ◽  
Marco Antonio Márquez Godoy ◽  
Luz Marina Ocampo Carmona
Keyword(s):  
Energy Source ◽  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Sulphate ◽  
Mining Waste ◽  
Acidithiobacillus Thiooxidans ◽  
Secondary Products ◽  
External Energy ◽  
Leptospirillum Ferrooxidans ◽  
External Energy Source ◽  
High Concentrations

One of the main characteristics of the microorganisms used in the leaching process is their capacity to adapt to aggressive environments, characterized by a notable presence of heavy metals. In this study the adaptation of the strains Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans was evaluated on a sphalerite concentrate from mining waste. In the adaptation tests, the energy source (ferrous sulphate) was gradually replaced by percentages of mineral pulp, ending with subcultures without the addition of an external energy source. The results show that the strains A. ferrooxidans and A. thiooxidans are more resistant to high concentrations of sphalerite, compared to the strain of L. ferrooxidans, since, in the case of this strain, it was necessary to repeat some tests (8% of pulp), since a deficient development was evident. This was associated with factors such as the decrease of the Fe+2 energy source, the increase of the pulp density, the accumulation of toxic metals and secondary products of the dissolution of minerals and the increase of the pH.

scholarly journals Effects of Conventional Flotation Frothers on the Population of Mesophilic Microorganisms in Different Cultures

Processes ◽  
2019 ◽  
Vol 7 (10) ◽  
pp. 653 ◽  
Author(s):  
Mohammad Jafari ◽  
Mehdi Golzadeh ◽  
Sied Shafaei ◽  
Hadi Abdollahi ◽  
Mahdi Gharabaghi ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Acidithiobacillus Ferrooxidans ◽  
Lower Sensitivity ◽  
Acidithiobacillus Thiooxidans ◽  
Methyl Isobutyl ◽  
Flotation Process ◽  
Leptospirillum Ferrooxidans ◽  
Pine Oil ◽  
Pure Cultures ◽  
Extraction Of Metals

Bioleaching is an environment-friendly and low-investment process for the extraction of metals from flotation concentrate. Surfactants such as collectors and frothers are widely used in the flotation process. These chemical reagents may have inhibitory effects on the activity of microorganisms through a bioleaching process; however, there is no report indicating influences of reagents on the activity of microorganisms in the mixed culture which is mostly used in the industry. In this investigation, influences of typical flotation frothers (methyl isobutyl carbinol and pine oil) in different concentrations (0.01, 0.10, and 1.00 g/L) were examined on activates of bacteria in the mesophilic mixed culture (Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans, and Acidithiobacillus thiooxidans). For comparison purposes, experiments were repeated by pure cultures of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans in the same conditions. Results indicated that increasing the dosage of frothers has a negative correlation with bacteria activities while the mixed culture showed a lower sensitivity to the toxicity of these frothers in comparison with examined pure cultures. Outcomes showed the toxicity of Pine oil is lower than methyl isobutyl carbinol (MIBC). These results can be used for designing flotation separation procedures and to produce cleaner products for bio extraction of metals.

scholarly journals Efecto de la cisteína en un proceso de biodepiritización de carbones en lecho empacado

2016 ◽  
Vol 18 (1) ◽  
Author(s):  
Gerardo Andrés Caicedo Pineda ◽  
Marco Antonio Márquez Godoy
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Acidithiobacillus Thiooxidans

<p>Se llevaron a cabo procesos de biodesulfurización de dos carbones colombianos ricos en azufre (“Mina Vieja” y “Vampiro”), en reactores de lecho empacado a nivel de erlenmeyer, utilizando un consorcio de Acidithiobacillus ferrooxidans (ATCC 23270) y Acidithiobacillus thiooxidans (ATCC 15494), evaluando la adición de cisteína a la solución lixiviante. Los ensayos fueron monitoreados por medidas de hierro en solución, pH y potencial redox. Adicionalmente, se hicieron análisis mineralógicos por difracción de rayos X (DRX) antes y después de los experimentos. Los ensayos sin adición de cisteína alcanzaron una oxidación de pirita de 45.3% y 57.9% para “Mina Vieja” y “Vampiro” respectivamente. Cuando se adicionó cisteína, la oxidación aumentó en 14.9% para “Mina Vieja” y 6.4% para “Vampiro”. Por otra parte, todos los ensayos evidenciaron remoción de caolinita, debido a su interacción con el ácido sulfúrico del medio. Con base en los resultados obtenidos, los componentes del carbón influenciaron tanto crecimiento bacteriano como la eficiencia de la cisteína sobre el grado de pirita oxidada.</p>

Rapid and specific detection of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans by PCR

2008 ◽  
Vol 92 (3-4) ◽  
pp. 102-106 ◽  
Author(s):  
Blanca Escobar ◽  
Karina Bustos ◽  
Gabriela Morales ◽  
Oriana Salazar
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Specific Detection ◽  
Leptospirillum Ferrooxidans

Experimental Research on Coal Biodesulfurization by Mixed Culture Column Leaching

2012 ◽  
Vol 512-515 ◽  
pp. 2500-2504 ◽  
Author(s):  
Yu Yang ◽  
Gai Mei Ren ◽  
Xiang Wang ◽  
Li Yang
Keyword(s):  
Mixed Culture ◽  
Acidithiobacillus Thiooxidans ◽  
Total Sulfur ◽  
Desulfurization Rate ◽  
Column Leaching ◽  
Leptospirillum Ferrooxidans ◽  
Coal Desulfurization ◽  
Inner Diameter ◽  
Lower Ratio ◽  
Sulfur In Coal

The removal of sulfur in coal before its combustion can reduce its environmental pollution, and microbial desulfurization should have good application prospects. Some species of bacteria have been used in the process of coal desulfurization, and our previous study demonstrated that mixed culture should be more efficient than pure culture by shaking cultivation. In this study, column leaching was investigated, due to its similarity to the industrial heap leaching. The mixed culture consisted of three strains of bacteria, which were Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans respectively. The ratio of the three strains of bacteria was 1: 1: 1. The column (50 cm height, 650 mm Inner Diameter) bioleaching experiments were carried out at ambient temperature. After desulfurization for 18 days, the ultimate desulfurization rate was 28.66% for total sulfur, which was higher than the previous study of J.Cara (with total sulfur desulfurization rate for 24% after 125 days). This study showed that ferric ions should be in proper concentration, and Leptospirillum ferrooxidans might be in lower ratio for reducing the precipitation of jarosite and improving the efficient of coal desulfurization.

scholarly journals Evidence for a Functional Quorum-Sensing Type AI-1 System in the Extremophilic Bacterium Acidithiobacillus ferrooxidans

2005 ◽  
Vol 71 (11) ◽  
pp. 7033-7040 ◽  
Author(s):  
Carolina Farah ◽  
Mario Vera ◽  
Danièle Morin ◽  
Dominique Haras ◽  
Carlos A. Jerez ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Genetic Locus ◽  
Metal Sulfide ◽  
Open Reading Frames ◽  
Functional Type ◽  
Sensing System ◽  
Reverse Transcription Pcr ◽  
Quorum Sensing System ◽  
Chemolithotrophic Bacteria

ABSTRACT Acidithiobacillus ferrooxidans is one of the main acidophilic chemolithotrophic bacteria involved in the bioleaching of metal sulfide ores. The bacterium-mineral interaction requires the development of biofilms, whose formation is regulated in many microorganisms by type AI-1 quorum sensing. Here, we report the existence and characterization of a functional type AI-1 quorum-sensing system in A. ferrooxidans. This microorganism produced mainly acyl-homoserine lactones (AHL) with medium and large acyl chains and different C-3 substitutions, including 3-hydroxy-C8-AHL, 3-hydroxy-C10-AHL, C12-AHL, 3-oxo-C12-AHL, 3-hydroxy-C12-AHL, C14-AHL, 3-oxo-C14-AHL, 3-hydroxy-C14-AHL, and 3-hydroxy-C16-AHL. A quorum-sensing genetic locus that includes two open reading frames, afeI and afeR, which have opposite orientations and code for proteins with high levels of similarity to members of the acyl synthase (I) and transcriptional regulator (R) protein families, respectively, was identified. Overexpression of AfeI in Escherichia coli and the associated synthesis of AHLs confirmed that AfeI is an AHL synthase. As determined by reverse transcription-PCR, the afeI and afeR genes were transcribed in A. ferrooxidans. The transcription levels of the afeI gene were higher in cells grown in sulfur and thiosulfate media than in iron-grown cells. Phosphate starvation induced an increase in the transcription levels of afeI which correlated with an increase in AHL levels. Two afe boxes which could correspond to the AfeR binding sites were identified upstream of the afeI gene. This is the first report of a functional type AI-1 quorum-sensing system in an acidophilic chemolithotrophic microorganism, and our results provide a very interesting opportunity to explore the control and regulation of biofilm formation during the bioleaching process.

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