scholarly journals In vitro kinetics of reticulocyte subtypes: maturation after red blood cell storage in additive solution-1 (AS-1)

2018 ◽  
Vol 40 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Adriana Urbina ◽  
Fernando Palomino
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Additive Solution ◽  
Kinetics Of ◽  
In Vitro Kinetics ◽  
Cell Storage

In vitro evaluation of di(2-ethylhexyl)terephthalate-plasticized polyvinyl chloride blood bags for red blood cell storage in AS-1 and PAGGSM additive solutions

Transfusion ◽  
2018 ◽  
Vol 58 (5) ◽  
pp. 1100-1107 ◽  
Author(s):  
Sharon Graminske ◽  
Kathleen Puca ◽  
Anna Schmidt ◽  
Scott Brooks ◽  
Amanda Boerner ◽  
...  
Keyword(s):  
Blood Cell ◽  
Polyvinyl Chloride ◽  
Red Blood Cell ◽  
In Vitro Evaluation ◽  
Cell Storage

Hemoglobinuria and Mechanical Hemolysis Associated with Red Blood Cell Transfusion in Pediatric Patients

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 3429-3429 ◽  
Author(s):  
Janet McNaughton ◽  
Jonathan Hughes ◽  
Jennifer C. Andrews ◽  
Tracy I. George ◽  
Cassandra Bergero ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Conflicts Of Interest ◽  
Infusion Pump ◽  
Red Blood Cell Transfusion ◽  
Treatment Center ◽  
Outpatient Unit ◽  
Time Period ◽  
Additive Solution

Abstract Abstract 3429 Introduction: Mechanical hemolysis is a non-immune mediated destruction of red blood cells that can be associated with blood transfusion. Various etiologies include the use of blood warmers, small bore needles or high infusion rates. We report the investigation of hemoglobinuria cases observed post-packed red blood cell (PRBC) transfusion with subsequent changes in management. Case report: Over a time period of 2 months, 6 events of hemoglobinuria and hemolysis were reported in 5 patients in the pediatric hematology/oncology treatment center. Each child presented with abnormally tea-colored urine following PRBC transfusion. Transfusion reaction workup included a direct antiglobulin test (DAT), urinalysis (UA), serum haptoglobin (H), lactate dehydrogenase (LDH), and total bilirubin (T-B). All patients had a negative or unchanged DAT, negative RBC antibody screen, decreased H, increased T-B, and increased LDH (4 out of 6), suggestive of intravascular hemolysis (Table). In spite of hemolysis, hemoglobin (Hgb)/hematocrit(Hct) increased normally. Multiple factors were investigated to determine a cause: collection and processing of blood units; handling of blood at the transfusion service; and blood administration by nursing staff. The only significant change identified was the implementation of a new infusion pump (pump A) replacing a model that was phased out. A hospital-wide retrospective review of urinalysis (UA) was performed over a one month time period, along with prospective UA surveillance in the outpatient unit. Results: In the 6 cases, a total of 10 irradiated PRBC units (7 with citrate phosphate dextrose buffer (CPDA1, HCT 65–80%) and 3 with additive solution (AS-5, HCT 55–65%)) were transfused. Although Pump A had been validated by the manufacturer with non-irradiated additive solution units, an in vitro study with irradiated CPDA1 RBC units was performed to compare pump A with an alternative pump B. Samples were taken after infusion using the pumps only (no needle or catheter) and dripped directly into the test tube for measurement of free hemoglobin (FHb). Irradiated CPDA1 units infused at a low rate (50ml/hr) showed an increase in FHb level at 1998mg/dL with pump A versus 496 mg/dL with pump B. Non-irradiated AS-5 units tested as controls resulted in a FHb level at 246mg/dL with pump A versus 117mg/dL with pump B. CPDA1 units were subsequently replaced with AS-5 units for transfusion. Finally, pump A was replaced by pump B in the outpatient hematology oncology unit. In vitro studies are still ongoing to determine if irradiation of the PRBC may also play a role in the mechanical hemolysis observed with pump A. One month retrospective UA review found no association between patients with hemoglobinuria and RBC transfusion. The UA prospective surveillance performed in the outpatient unit revealed that 7 of 26 patients had trace hemoglobinuria. Each one had a confounding factor (chronic hemolysis) for hemoglobinuria. Conclusion: Preliminary investigation determined that the infusion of highly concentrated irradiated RBC (CDPA1) using a specific commercial pump was associated with mechanical hemolysis. The change to less concentrated RBC units through an alternative pump has been to date, an effective corrective action. Disclosures: No relevant conflicts of interest to declare.

Influence of irradiation on in vitro red-blood-cell (RBC) storage variables of leucoreduced RBCs in additive solution PAGGS-M

Vox Sanguinis ◽  
2010 ◽  
Vol 101 (1) ◽  
pp. 21-27 ◽  
Author(s):  
B. Hauck ◽  
D. Oremek ◽  
R. Zimmermann ◽  
R. Ruppel ◽  
B. Troester ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Additive Solution

Exploratory in vitro study of red blood cell storage containers formulated with an alternative plasticizer

Transfusion ◽  
2011 ◽  
Vol 52 (7) ◽  
pp. 1439-1445 ◽  
Author(s):  
Larry J. Dumont ◽  
Sharry Baker ◽  
Deborah F. Dumont ◽  
Louise Herschel ◽  
Susan Waters ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Storage Containers ◽  
Cell Storage

scholarly journals Sodium activation kinetics of red blood cell Na+/Li+ countertransport in diabetes: methodology and controversy.

1992 ◽  
Vol 3 (4) ◽  
pp. S41
Author(s):  
M Canessa ◽  
G Zerbini ◽  
L M Laffel
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
Activation Kinetics ◽  
Renal Complications ◽  
Insulin Dependent ◽  
Sodium Activation ◽  
Kinetics Of

Although many studies report an elevated Vmax of red blood cell Na/Li countertransport (CTT) activity in patients with insulin-dependent diabetes mellitus (IDDM) complicated by renal disease, divergent reports exist. This article reviews the technical issues and selection criteria that fuel this controversy. In addition, new studies from this laboratory indicate that insulin in vitro and in the nonfasted state modulate CTT activity and may contribute to the discrepant findings. Incubation of red blood cells from fasted controls with physiologic concentrations of insulin induced a twofold increase in the Km for external Na+. Similarly, Na+ activation kinetics of Li+ efflux showed saturation between 50 and 150 mM Na+ in fasted controls whereas saturation, postprandially, occurred between 100 and 150 mM Na+ as a result of an increase in Km. To clarify the role of prandial status on the measurement of Na+/Li+ CTT activity in diabetes, Na+ activation kinetics were investigated in 34 nonfasting patients with IDDM. Li+ efflux was fully saturated between 80 and 150 mM Na+ in the normoalbuminuric subjects (N = 22), whereas saturation occurred between 150 and 280 mM Na+ in the patients with diabetic nephropathy (N = 14). Patients with nephropathy have higher values of Km for Na+ than do the patients free of renal complications (86 +/- 9.5 versus 41.3 +/- 3.4 mM Na+, respectively; P < 0.000012). The higher Km prevented complete saturation of Li+ efflux at 150 mM extracellular Na+ concentration and contributed to the underestimation of Vmax at 150 mM Na+ selectively in persons with renal complications.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Red blood cell storage in additive solution-7 preserves energy and redox metabolism: a metabolomics approach

Transfusion ◽  
2015 ◽  
Vol 55 (12) ◽  
pp. 2955-2966 ◽  
Author(s):  
Angelo D'Alessandro ◽  
Travis Nemkov ◽  
Kirk C. Hansen ◽  
Zbigniew M. Szczepiorkowski ◽  
Larry J. Dumont
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Redox Metabolism ◽  
Additive Solution ◽  
Cell Storage

Red blood cell storage increases hypoxia-induced nitric oxide bioavailability and methemoglobin formation in vitro and in vivo

Transfusion ◽  
2014 ◽  
Vol 54 (12) ◽  
pp. 3178-3185 ◽  
Author(s):  
Emre Almac ◽  
Rick Bezemer ◽  
Petra M. Hilarius-Stokman ◽  
Peter Goedhart ◽  
Dirk de Korte ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Methemoglobin Formation ◽  
Nitric Oxide Bioavailability ◽  
Cell Storage

scholarly journals Influence of prestorage leukoreduction and subsequent irradiation on in vitro red blood cell (RBC) storage variables of RBCs in additive solution saline-adenine-glucose-mannitol

Transfusion ◽  
2009 ◽  
Vol 49 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Robert Zimmermann ◽  
Simone Wintzheimer ◽  
Volker Weisbach ◽  
Julian Strobel ◽  
Juergen Zingsem ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Solution Saline ◽  
Additive Solution
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