scholarly journals Novel in vitro model of urea cycle disorder using citrullinemia type I patient-derived iPSC s

HPB ◽  
2016 ◽  
Vol 18 ◽  
pp. e300
Author(s):  
E.Y. Yoshitoshi ◽  
T. Toyoda ◽  
K. Yasuda ◽  
M. Kotaka ◽  
K. Okita ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Urea Cycle ◽  
Urea Cycle Disorder ◽  
Type I ◽  
Vitro Model ◽  
Citrullinemia Type I

A Simple In Vitro Model of Mechanical Injury of Confluent Cultured Endothelial Cells to Study Quantitatively the Repair Process

1986 ◽  
Vol 56 (02) ◽  
pp. 232-235 ◽  
Author(s):  
Claude Klein-Soyer ◽  
Alain Beretz ◽  
Régine Millon-Collard ◽  
Joseph Abecassis ◽  
Jean-Pierre Cazenave
Keyword(s):  
Endothelial Cells ◽  
In Vitro Model ◽  
Repair Process ◽  
Mechanical Injury ◽  
Brain Extract ◽  
Type I ◽  
Culture Dish ◽  
Vitro Model ◽  
Adhesive Proteins

SummaryA model of in vitro mechanical injury of confluent human endothelial cells (EC) in culture was developed. Human EC were obtained from umbilical veins and grown to confluence. Application on the EC monolayer of a calibrated disk of cellulose poly acetate paper resulted in removal of the EC, leaving a continuous subendothelial extracellular matrix (ECM) on the culture dish. The regeneration time depended on the original size of the lesion. Regeneration was similar with EC grown on different substrates such as human fibronectin, human subendothelial ECM, bovine collagen type I or surfaces coated with Transglutine®, a surgical glue containing adhesive proteins. A human brain extract containing growth factor activity accelerated significantly the repair of the lesion, especially at low serum concentration. This simple in vitro model of mechanical injury allows the quantitative study of the effects of matrices, growth factors and pharmacological agents on the repair process.

scholarly journals Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

2020 ◽  
Vol 11 (1) ◽  
pp. 3 ◽  
Author(s):  
Yun-Mi Kang ◽  
Chul-Hee Hong ◽  
Sa-Haeng Kang ◽  
Dong-Seok Seo ◽  
Seong-Oh Kim ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Type I Collagen ◽  
Dermal Fibroblasts ◽  
Epidermal Keratinocytes ◽  
Type I ◽  
Mrna Levels ◽  
Protective Effects ◽  
Lactobacillus Buchneri ◽  
Vitro Model

Ultraviolet (UV) exposure triggers the abnormal production of reactive oxygen (ROS) species and the expression of matrix metalloproteinases (MMPs) that are responsible for photoaging. Probiotics are widely used in healthcare and for immune enhancement. One probiotic, Lactobacillus buchneri is found in Kimchi. This study was aimed at assessing the anti-photoaging effect of plant extracts fermented with L. buchneri (PELB) to develop functional cosmetics. We investigated the anti-photoaging effect of PELB in a UVB-induced photoaging in vitro model and selected effective extracts using the elastase inhibition assay, ELISA for Type I procollagen and collagenase-1, and quantitative real time PCR. Normal human dermal fibroblasts and epidermal keratinocytes were pre-treated with PELB and exposed to UVB. We found that PELB decreased elastase activity and increased type I collagen expression in a UVB-induced photoaging in vitro model. In addition, PELB greatly reduced collagenase activity and MMP mRNA levels in a UVB-induced photoaging in vitro model. Furthermore, PELB promoted the expression of moisture factor and anti-oxidant enzymes in a UVB-induced photoaging in vitro model. These results indicated that the PELB could be potential candidates for the protective effects against UVB-induced photoaging. Overall, these results suggest that PELB might be useful natural components of cosmetic products.

scholarly journals The R3/1 alveolar epithelial type I cell line ‐ Characterisation as an in vitro model for drug disposition

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Carsten Ehrhardt ◽  
Sibylle Endter ◽  
Rikke Andersen ◽  
Roland Koslowski ◽  
Lenka Horalkova
Keyword(s):  
Cell Line ◽  
In Vitro Model ◽  
Drug Disposition ◽  
Type I ◽  
Alveolar Epithelial ◽  
Vitro Model

1042: A New in vitro Model for Cystinuria Type I by RNAI Transfection

2006 ◽  
Vol 175 (4S) ◽  
pp. 335-335
Author(s):  
Sreedhar Sagi ◽  
Yanwei Gao ◽  
Axel Haecker ◽  
Peter Aiken ◽  
Maurice S. Michel ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Type I ◽  
Vitro Model

75 GENERATION OF AN OPTIMIZED STABLE IN VITRO MODEL OF HUMAN CYSTINURIA TYPE I USING RETROVIRAL VECTORS.

2007 ◽  
Vol 6 (2) ◽  
pp. 41
Author(s):  
S. Sagi ◽  
M. Picard-Maureau ◽  
G. Wendt-Nordahl ◽  
P. Krombach ◽  
A. Hacker ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Retroviral Vectors ◽  
Type I ◽  
Vitro Model

A serum-free in vitro model of renal microvessel development

1998 ◽  
Vol 274 (6) ◽  
pp. F1150-F1160 ◽  
Author(s):  
Lisa M. Antes ◽  
Monica M. Villar ◽  
Sylvia Decker ◽  
Roberto F. Nicosia ◽  
Dean A. Kujubu
Keyword(s):  
Growth Factors ◽  
Endothelial Cell ◽  
In Vitro Model ◽  
Type I Collagen ◽  
Type I ◽  
Renal Vasculature ◽  
Serum Free ◽  
Polypeptide Growth Factors ◽  
Vitro Model

The differentiation and organization of the embryonic renal vasculature is a crucial event in renal development. To study this process, we developed a serum-free in vitro model of renal microvessel development. Mouse embryonic kidney explants, when embedded specifically in type I collagen, demonstrate outgrowth of microvascular structures when stimulated by the phorbol ester 12- O-tetradecanoylphorbol 13-acetate (TPA, 10–50 ng/ml). Other polypeptide growth factors stimulated little, if any, microvessel outgrowth from the explants. Similar outgrowths were not observed when other embryonic tissue explants were used. The number of microvessels observed depended on the gestational age of the explants. We hypothesize that TPA induces the in situ differentiation of metanephric mesenchymal cells into endothelial cell precursors and that specific matrix proteins and cell-matrix interactions are necessary for the organization of these precursors into microvessels. Our model will allow us to examine in detail the responsiveness of metanephric kidney cells to both growth factors and extracellular matrix molecules and to understand how they influence renal endothelial cell differentiation.

scholarly journals Autologous Microfragmented Adipose Tissue Reduces the Catabolic and Fibrosis Response in an In Vitro Model of Tendon Cell Inflammation

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Marco Viganò ◽  
Gaia Lugano ◽  
Carlotta Perucca Orfei ◽  
Alessandra Menon ◽  
Enrico Ragni ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
In Vitro Model ◽  
Collagen Type ◽  
Cyclooxygenase 2 ◽  
Type I ◽  
Collagen Type Iii ◽  
Tendon Cell ◽  
Type Iii ◽  
Vitro Model

Background. Mesenchymal stem cells (MSCs) emerged as a promising therapy for tendon pathologies. Microfragmented adipose tissue (μFAT) represents a convenient autologous product for the application of MSC-based therapies in the clinical setting. In the present study, the ability of μFAT to counteract inflammatory processes induced by IL-1β on human tendon cells (TCs) was evaluated. Methods. Cell viability and proliferation were evaluated after 48 hours of transwell coculture of TCs and autologous μFAT in the presence or absence of IL-1β. Gene expression of scleraxis, collagen type I and type III, metalloproteinases-1 and -3, and cyclooxygenase-2 was evaluated by real-time RT-PCR. The content of VEGF, IL-1Ra, TNFα, and IL-6 was evaluated by ELISA. Results. IL-1β-treated TCs showed augmented collagen type III, metalloproteases, and cyclooxygenase-2 expression. μFAT was able to reduce the expression of collagen type III and metalloproteases-1 in a significant manner, and at the same time, it enhanced the production of VEGF, IL-1Ra, and IL-6. Conclusions. In this in vitro model of tendon cell inflammation, the paracrine action of μFAT, exerted by anti-inflammatory molecules and growth factors, was able to inhibit the expression of fibrosis and catabolic markers. Then, these results suggest that the application of μFAT may represent an effective conservative or adjuvant therapy for the treatment of tendon disorders.

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